LOCUS       sequence-43558-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE4c-RFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      154..225
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      241..268
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(506..525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
       61 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
      121 aggatccaaa ctcgagtaag gatctccagg catcaaataa aacgaaaggc tcagtcgaaa
      181 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      241 ggctcacctt cgggtgggcc tttctgcgtt tatacctagg gcgttcggct gcggcgagcg
      301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      601 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      661 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      721 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      781 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      841 tggcctaact acggctacac
//
