LOCUS       sequence-435610-       11519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-associated transposases from V. cholerae and used for RNA 
            guided cargo insertion in Vibrio natriegens.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Xu J, Sun Y, Wu J, Yang S, Yang L
  TITLE     Chromosome recombination and modification by LoxP-mediated evolution
            in Vibrio natriegens using CRISPR-associated transposases.
  JOURNAL   Biotechnol Bioeng. 2024 Mar;121(3):1163-1172. doi: 
            10.1002/bit.28639. Epub 2023 Dec 22.
  PUBMED    38131162
REFERENCE   2  (bases 1 to 11519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.28639";
            journalName: "Biotechnol Bioeng"; date: "2024-03"; volume: "121"; 
            issue: "3"; pages: "1163-1172"
COMMENT     Sequence Label: pTNSQCas
FEATURES             Location/Qualifiers
     source          1..11519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6475..6486
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7913..7931)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7927..7974
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(8246..8905)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8820..8839
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8906..9008)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(9285..9302)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9419..9964)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9499..9518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10260..10886)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     10791..10810
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        10902..10957
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    10908..10926
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    10938..10956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tcccctgctg tcctttgtgt ctgcgagaaa atggctacgc ctcctacctt tggcactttc
       61 aggggtacga atactgccac agccataacg tacctttaat taccacttgt agctgtggta
      121 aggagtttga ctaccgagta tctgggttaa agggcatttg ctgcaaatgc aaggagccta
      181 tcaccttaac cagcagggag aacggtcatg aggcagcgtg tactgtttca aactggcttg
      241 ctggccatga atctaaacct ctgccaaatc ttcctaaaag ctaccgatgg ggtttagttc
      301 attggtggat gggtattaaa gatagcgagt tcgatcactt ttcgttcgtt caatttttct
      361 caaactggcc aaggtcattc cactcgataa tcgaagatga agtagagttc aaccttgagc
      421 atgctgttgt cagcacgtct gaattacgac taaaagatct tcttggtcga ttgtttttcg
      481 gttcaattcg gttacctgag cggaatcttc aacacaatat catccttggt gagcttctct
      541 gctatttaga aaatcgttta tggcaagaca agggattaat cgccaacctc aaaatgaacg
      601 cgttagaggc gactgtaatg ttaaattgta gcctcgatca gattgcatca atggttgaac
      661 aacgcatctt gaagccaaat cgaaaaagca agcccaacag ccctcttgat gttaccgatt
      721 atctatttca tttcggcgat attttctgtc tttggttagc tgagttccaa agcgatgagt
      781 ttaaccgttc gttttatgtg tcgaggtggt aaatgcaaac tctgaaagaa ctaatcgcat
      841 ccaatcctga cgacttaaca actgaactta agagagcatt tcgtccactc acaccgcata
      901 ttgcaattga tggtaatgaa cttgacgcac tgacgatatt agtcaattta accgataaga
      961 ctgatgatca gaaagacctg ctcgatcgag ccaaatgcaa gcaaaaactt cgagatgaaa
     1021 aatggtgggc tagctgcata aattgcgtta attacagaca aagccataac ccaaaattcc
     1081 cggatatacg ttctgaaggc gtgattcgaa cccaagccct gggtgaatta ccgagtttcc
     1141 tactctcgtc ctcaaaaatc ccaccatacc attggtcata tagccatgat tcaaaatacg
     1201 tcaacaaaag cgcattcctc accaatgagt tttgttggga tggtgagatc tcatgtttgg
     1261 gtgagcttct taaagatgca gatcacccac tttggaatac tttgaaaaag ttaggttgtt
     1321 ctcaaaaaac ttgcaaagca atggcaaaac aactagctga tattactctc acgactatca
     1381 atgtcacact cgcaccaaat tacctgactc aaatatctct ccccgatagt gatacatcct
     1441 acatttcact ctctcctgtt gcatcgctat cgatgcaaag ccactttcat cagaggctcc
     1501 aagatgaaaa tcggcatagc gcgataacgc ggtttagccg aactaccaac atgggggtca
     1561 ctgcgatgac atgtggcggc gcatttagaa tgttaaagtc tggcgctaag ttttccagcc
     1621 cccctcatca ccgattaaac agtaaacgaa gttggttgac gtcagagcat gttcagtcat
     1681 taaaacagta ccagcgcctc aacaaaagcc tcatacctga aaactctcgg attgcactcc
     1741 gtagaaaata caaaatcgag cttcaaaata tggtcagatc ttggtttgca atgcaagacc
     1801 atacccttga ttcgaacata cttatccaac atttgaatca tgacctatct tacttaggag
     1861 ccacaaaacg ttttgcatac gatccagcga tgaccaagct ctttactgag cttttgaaac
     1921 gagagttatc aaattcaatc aataatggtg agcaacacac taatggatcg tttttagtcc
     1981 taccgaatat cagggtttgt ggcgcaacag ctttaagctc cccggtaacg gtggggattc
     2041 catcacttac agctttcttt ggcttcgttc acgcatttga acggaatata aatcgcacca
     2101 cctcatcgtt tcgtgttgaa tcctttgcga tatgcgtcca tcaactacat gtcgagaagc
     2161 gaggtttgac agcagagttt gtggaaaaag gcgacgggac tatatccgct cccgcgaccc
     2221 gggatgactg gcagtgtgat gtcgtattta gccttatttt gaacaccaac tttgctcaac
     2281 atattgacca agatacgtta gttacatcac taccaaagcg attggctcgg ggttcagcaa
     2341 aaattgcgat tgatgacttt aaacatatca actcattctc gacattagaa acagcgatcg
     2401 aatctctgcc aatagaagct ggtaggtggt tatcacttta cgcacagtca aacaataatc
     2461 taagtgatct attagcagcc atgacagagg accatcagct catggcaagc tgcgtcggtt
     2521 accacttgtt agaagagccc aaagataaac caaactccct cagaggttac aaacacgcta
     2581 tcgccgagtg catcattgga ctcattaact caatcacctt tagctcagag actgatccca
     2641 acacaatctt ttggtcgcta aagaactatc aaaactacct agtggtacag ccaaggagta
     2701 tcaacgatga aactaccgac aaatctagcc tatgagcgct ctatcgaccc atcagatgtc
     2761 tgtttttttg tcgtctggcc cgatgataga aaaacacctt taacctacaa ttctcgtact
     2821 ctgctcgggc aaatggaagc ggcatcatta gcctatgatg tctcaggtca accaataaaa
     2881 agtgccaccg ctgaggcgtt agctcaaggg aaccctcatc aagttgattt ctgccacgtt
     2941 ccatacggtg cgagtcatat tgaatgcagt ttctccgtct cgttttcttc tgaactacgt
     3001 caaccatata agtgtaactc aagcaaagtt aaacaaacgc tagtgcaatt agtcgagctc
     3061 tacgaaacga aaatcggctg gactgagcta gcaacccgat atttgatgaa catttgcaac
     3121 ggtaaatggc tgtggaaaaa tacccgtaaa gcctattgct ggaacattgt acttacacct
     3181 tggccttgga acggggaaaa ggttggattt gaagatattc gtactaacta cacctcacgg
     3241 caagacttta aaaataataa aaattggtct gctatagttg aaatgatcaa aaccgcattt
     3301 tctagtactg atgggctggc gatatttgaa gtcagggcca ccttgcactt gccaacgaat
     3361 gctatggtgc ggccaagcca agttttcaca gaaaaagaaa gtggcagtaa aagtaaatct
     3421 aaaactcaaa acagtcgagt ttttcagagt acaactattg atggtgaacg atcgccaata
     3481 ctaggggcct ttaaaacggg agcagctatt gcaaccattg acgactggta tcctgaagcc
     3541 actgagccac taagggtcgg acggtttggg gttcatcgcg aagatgtcac ttgctaccgt
     3601 catccgtcta ccggaaaaga ttttttctcg atattacaac aagcagagca ctatattgaa
     3661 gtgttgagcg ccaacaaaac tcccgctcaa gaaactatca acgacatgca ctttttaatg
     3721 gctaacctga ttaagggtgg gatgttccag cataaaggag actgactgtg aaatggtatt
     3781 ataagacaat cacctttctg ccagagttgt gcaacaacga gtcactggct gcaaagtgtc
     3841 tccgcgttct gcatggattt aactatcagt atgagacacg aaatatcggc gtttcatttc
     3901 cgctttggtg tgatgcaacg gttggaaaaa agatttcatt tgtcagcaag aacaagatag
     3961 aactcgactt actacttaaa caacactatt tcgtccaaat ggaacaactt caatactttc
     4021 atatatccaa cactgttctc gtcccagaag attgtacata cgtttccttt agacgctgtc
     4081 aatctataga taagctcaca gcagcagggc tggcaaggaa aatcagacgc ctggagaaac
     4141 gtgctctatc tagaggcgag caatttgacc catcatcttt tgctcaaaaa gagcatactg
     4201 caatagcgca ctaccactca cttggggagt ccagcaaaca gacgaaccgc aactttcgac
     4261 tcaatatcag gatgctctcg gagcaacccc gtgaggggaa ctcgattttt agtagctatg
     4321 gcttatcaaa ttcagaaaac tcgtttcagc ctgtaccctt aatctgacct aggctgcttt
     4381 taaaaggaga tatagcatgg cgacaagttt acctacgccc tcagcaatta cgacttcggc
     4441 gttagagtat gcattccata ctcccgctcg caatctaacg aaatctcgcg gaaaaaacat
     4501 tcatcgttat gtcagtgtaa agatgagtaa gaggattacg gtagaatcta ctctagagtg
     4561 tgatgcctgc tatcactttg attttgagcc aagtattgtt cgcttttgcg ctcaaccgat
     4621 tcgattttta tattatctca atggtcagtc tcactcctat gttcctgact ttctagttca
     4681 atttgatacc aacgagtttg ttctatatga agtaaagtca gcttatgcta agaacaaacc
     4741 tgattttgat gttgaatggg aggcgaaagt aaaagcagca actgaactag ggctagaatt
     4801 ggagcttgtt gaagagagtg atattaggga tacggttgta ttaaataatc ttaagcgcat
     4861 gcatcgttat gcttcgaaag atgagctgaa taacgtacat aactctctct taaaaataat
     4921 aaagtacaat ggcgcccaat ctgcaagatg cttgggagaa cagttgggtt taaaaggccg
     4981 aactgtttta ccaattttgt gcgatttgct gtcaaggtgt ttactcgata cacgtttgga
     5041 taagcctcta tctcttgaat ctcgatttga gttggccagt tatggctaag aaagggttct
     5101 caagtttcca tagaaaagca gtctcttccc aagatacgct tgaatcgata gagcttgtct
     5161 ctagcgctaa ttgtctagaa agtgttacgt atcaagatat atcagcattt cccgaaacaa
     5221 ttgcggtaga gattaatttc cgattaagca ttcttcgctt tttagcgcga aagtgcgaaa
     5281 ccattgtggc caaatcaatt gaaccacatc gtgtagagct acagcaaaac tatagtagaa
     5341 aaatacccag tgcaataacg atatatcgat ggtggcttgc ttttcgaaaa tcagactaca
     5401 accccattag cttagcacct aatatcaagg atagaggtaa tagagagaca aaagtgtcaa
     5461 cagttgttga ttctattatg gaacaggcag ttgaaagagt tatatctgga cgaaaagtca
     5521 atgttagctc tgcatataaa cgtgttcgac gaaaagttcg tcaatacaat ctaactcatg
     5581 gaacgaaata cacgtatcct aagtacgaat ctgtaagaaa gcgagtaaaa aagaaaaccc
     5641 catttgagtt attagccgca gggaaagggg agagagtagc taagagagag tttcgccgaa
     5701 tgggaaaaaa gatcctcacg tctagcgtgc tagagagggt tgaaatagat cacactgtcg
     5761 ttgacctttt tgcagtacac gaagagtatc gaatcccatt gggccgacct tggcttactc
     5821 aattggttga ttgttacagt aaagctgtaa tcggttttta tttaggtttc gagcctccta
     5881 gctatgtgtc ggtttccctt gcacttaaga atgcaataca acgcaaagat gacttaatct
     5941 cctcgtatga atcgatcgag aatgaatggc tatgttatgg catcccagac ctactcgtaa
     6001 ctgataatgg taaagagttt ttgtcgaaag catttgatca agcatgtgaa tcactattga
     6061 tcaatgtgca tcaaaataaa gttgagacgc ccgacaacaa acctcatgtt gaacgtaact
     6121 acgggactat taatacttct ctgttagacg atttacctgg gaaatccttc agccagtacc
     6181 ttcaaagaga agggtacgac tctgtgggag aagctaccct tacactcaat gagattagag
     6241 aaatttactt aatttggttg gtggatattt atcataaaaa acccaatcag agaggcacta
     6301 attgtcctaa tgttgcgtgg aaaaagggtt gtcaagaatg ggaaccagag gagttctctg
     6361 gttctaaaga cgaattagac tttaaatttg ctattgttga ttacaaacaa cttactaaag
     6421 tagggataac tgtctacaaa gaactgagtt atagcaatga ccgtttagct gaatatagag
     6481 ggaagaaagg aaaccataaa gttcagttca agtataaccc tgagtgtatg gcagttattt
     6541 gggtgttgga tgaggatatg aatgagtact ttacagttaa tgcgattgac tacgaatatg
     6601 caagtagagt atcactttgg caacataaat ataacatgaa atatcaagca gaactaaatt
     6661 cagcagaata tgatgaggac aaggaaattg atgcagaaat aaaaattgaa gaaatcgcag
     6721 atcgttcaat tgttaagact aacaaaatca gagctcggag gcgtggcgct aggcatcaag
     6781 agaatagcgc aagggctaag tcaatcagta atgcgaaccc ggcctcgata caaaaacatg
     6841 aagatgaaat cgttagtgca gataatgacg attgggatat tgattatgtc tgagaaatcg
     6901 tcaaatgagt gaaacgcgtg aggctcgtat atcaagagct aaaagggcat ttgtatccac
     6961 accgagcgtt aggaaaatct tgagttacat ggatagatgt agagatctat cagacctaga
     7021 gtctgagcct acatgcatga tggtctatgg tgcttcgggt gtaggtaaaa cgaccgtcat
     7081 caagaaatac ttaaatcaaa acagaagaga gtccgaagcc gggggcgata taataccggt
     7141 tttgcatatt gagttgccag acaatgcgaa gccagtagat gcagcaaggg aattgctggt
     7201 tgaaatgggt gacccgctag cactttatga aactgactta gctagattga cgaaaagact
     7261 gactgaatta atccctgcgg tcggcgtgaa gctgattatt atcgatgagt tccaacattt
     7321 ggtggaagaa aggtcaaatc gggttcttac ccaagtaggt aattggctaa aaatgatact
     7381 taacaaaacg aaatgtccaa ttgttatatt tggtatgcca tactcaaaag ttgtactgca
     7441 agcaaactcg caacttcacg ggcgattttc cattcaggtt gaactgcgcc cctttagcta
     7501 ccagggaggt agaggtgtat ttaaaacttt tttggaatac cttgataaag ccctaccttt
     7561 tgaaaaacag gctggcttag ccaacgaaag tttgcagaaa aaattgtatg cattctctca
     7621 gggaaacatg cgttcgttga gaaaccttat ttatcaagca tctatcgaag caattgataa
     7681 tcagcatgag acgataaccg aagaagattt cgtttttgca tcgaagttga catcgggcga
     7741 taaacccaac tcatggaaaa atccttttga ggagggtgtt gaggtaacag aagatatgtt
     7801 acgaccgcca ccaaaagata ttggttggga ggactatttg agacattcaa ccccgagagt
     7861 gagtaaacca ggtagaaata aaaacttttt cgaataacct aggctgctgc caccgctgag
     7921 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     7981 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac
     8041 cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcatcg
     8101 tggccggatc ttgcggcccc tcggcttgaa cgaattgtta gacagtcgaa tttgctttcg
     8161 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca ggcgtttaag
     8221 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt
     8281 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     8341 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     8401 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     8461 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     8521 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     8581 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     8641 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     8701 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     8761 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     8821 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     8881 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     8941 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     9001 gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga
     9061 caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttta gctagctcac
     9121 tcggtcgcta cgctccgggc gtgagactgc ggcggtagcg gagtgtatac tggcttacta
     9181 tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca
     9241 ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg
     9301 ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc
     9361 ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt
     9421 tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc
     9481 gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct
     9541 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
     9601 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
     9661 gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9721 ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt
     9781 agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc
     9841 tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc
     9901 gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc
     9961 tcaagaagat catcttatta atcagataaa atatttctag ttttctactg aaccgctcta
    10021 gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagccagc acctgaagtc
    10081 agccagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt tagtcatgcc
    10141 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcc tgatgaatcc
    10201 cctaatgatt tttatcaaaa tcattaaggt taccatcacg gaaaaaggtt atgctgcttt
    10261 taagacccac tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg
    10321 aataagaagg ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat
    10381 ggcggcatac tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga
    10441 tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc
    10501 tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt
    10561 ttttctgtag gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca
    10621 catctaaaac ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg
    10681 caaaagtgag tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc
    10741 ttatttttta catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta
    10801 cgggttgtta aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact
    10861 ttacttttat ctaatctaga catcattaat tcctaatttt tgttgacact ctatcattga
    10921 tagagttatt ttaccactcc ctatcagtga tagagaaaag tgaactctag aaataatttt
    10981 gtttaacttt aaaaggagat ataccatggg tgaactgccg agtaggtagc tgataacgag
    11041 acctctggtc tcgtgaactg ccgagtaggt agctgataac ggatccgaat tcgagctcgg
    11101 cgcgcctgca ggtcgacaag cttgcggccg ctaaggagat atacatatgt ttttgcaaag
    11161 acctaaacct tacagcgatg aaagtttaga aagtttcttt atccgagtgg ctaacaaaaa
    11221 tggctacggt gatgtccatc gcttcctaga agccactaaa cgattccttc aagacattga
    11281 ccataatggc tatcaaacct ttccgactga tataactcgg ataaacccat actcagctaa
    11341 aaacagttcc agcgcacgaa ctgcgtcatt cctgaaactt gcacaattga catttaatga
    11401 accgccagag ctacttgggt tggcaattaa cagaacaaac atgaaatact cgccgtcaac
    11461 tagcgcggtt gttagaggtg cagaagtctt tcctcgcagt ttactacgga cgcactcca
//
