LOCUS       sequence-435616-        9101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral backbone plasmid to insert pegRNAs of interest. The 
            cloning site is BsmbI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: lenti-pegRNA-puro backbone
FEATURES             Location/Qualifiers
     source          1..9101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      341..372
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        complement(411..651)
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     complement(461..480)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(631..651)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(829..873)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1058..1291
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1785..1910
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1957..2137
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2138..2364)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2392..2412)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2392..2410)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2431..2447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2431..2447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2444..2466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2455..2471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2479..2485,2486..2503,2504..2509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2524..2545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2662..2679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2833..3421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2913..2932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3592..4383,4384..4452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4215..4234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4453..4557)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4583..5038)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4720..4741)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4932..4951
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5165..5187
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5179..5196
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5180..5196
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5206..5225
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5206..5224
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      5245..5380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5420..5541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5431..5450)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5485..5504
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5619..5852
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5924..6512
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6030..6041
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6439..6459
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6528..7127)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6611..6631)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7108..7127
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7140..8318)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7149..8087
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7172..7192)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8457..8574
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    8669..8687
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8694..8712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             8730..8741
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(8748..9101,1..324)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
       61 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
      121 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
      181 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
      241 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
      301 ggtcgtcact ccaccggtgc ttaatggcac tcctcagcca agtcaaaagc ctccgaccgg
      361 aggcttttga ctacggtctc gggtggagac gcgttgttgg cgcgcctttc gtcctttcca
      421 caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc
      481 cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca
      541 cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta tctctctaac
      601 agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct ctctgggtcc
      661 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
      721 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
      781 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
      841 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
      901 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
      961 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     1021 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     1081 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     1141 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     1201 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     1261 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     1321 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     1381 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
     1441 gcggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa
     1501 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
     1561 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
     1621 atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc
     1681 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
     1741 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
     1801 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
     1861 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
     1921 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
     1981 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     2041 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     2101 gctcccaggc tcagatctgg tctaaccaga gagacccgtt tatgtatcga gctaggcact
     2161 taaatacaat atctctgcaa tgcggcaatt cagtggttcg tccaatccat gtcagacccg
     2221 tctgttgcct tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac
     2281 ggtgcttttt ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact
     2341 acaagagtat tgcataagac tacattaagc ttgcagctcc agcttttgtt ccctttagtg
     2401 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2461 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2521 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2581 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2641 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2821 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3001 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3241 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3481 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3541 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3601 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3661 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3721 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3781 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3841 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3901 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3961 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4021 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4081 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4141 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4201 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4261 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4321 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4381 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4441 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4501 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4561 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     4621 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4681 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4741 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4801 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     4861 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4921 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4981 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     5041 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     5101 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     5161 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     5221 tagggcgaat tgggtacgtc cctaggcctc caaaaaagcc tcctcactac ttctggaata
     5281 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     5341 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
     5401 tagctagagc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     5461 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     5521 ataagctgca ataaacaagt tcctctcact ctctgatatt catttctttg caagttataa
     5581 atactgaata ataagatgac atgaactact atacgtactg ctagagattt tccacactga
     5641 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     5701 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     5761 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa aaagcagatc
     5821 ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta aaaagtggct
     5881 aagatctaca gctgccttgt aagtcattgg tcttaaagtc gacgcgggga ggcggcccaa
     5941 agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg cagagccggc
     6001 agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta gcagaaggac
     6061 gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga cgatgatttc
     6121 cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca gcagcgggca
     6181 aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa agtcccggaa
     6241 aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg
     6301 cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga cagcaaccag
     6361 gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg atacaaaggc
     6421 attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat accagtcaat
     6481 ctttcacaaa ttttgtaatc cagaggttga ttgtcgactt aacgcgttca ggcaccgggc
     6541 ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg
     6601 aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc
     6661 accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg
     6721 ccctggtggt cgggcgagac tccgacggtg gccaggaacc acgcgggctc cttgggccgg
     6781 tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg
     6841 gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc
     6901 cagaccgcca cggcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg
     6961 cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg
     7021 gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg
     7081 acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt ggccgtacgt
     7141 cacgacacct gaaatggaag aaaaaaactt tgaaccactg tctgaggctt gagaatgaac
     7201 caagatccaa actcaaaaag ggcaaattcc aaggagaatt acatcaagtg ccaagctggc
     7261 ctaacttcag tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc
     7321 caacctaaag acgacgtact ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc
     7381 cggtactccg tggagtcaca tgaagcgacg gctgaggacg gaaaggccct tttcctttgt
     7441 gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc tccattttga gctccctgca
     7501 gcagggccgg gaagcggcca tctttccgct cacgcaactg gtgccgaccg ggccagcctt
     7561 gccgcccagg gcggggcgat acacggcggc gcgaggccag gcaccagagc aggccggcca
     7621 gcttgagact acccccgtcc gattctcggt ggccgcgctc gcaggccccg cctcgccgaa
     7681 catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca
     7741 gtgtgcagat cttggcccgc atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca
     7801 ggtcatcaaa aattttaaat ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg
     7861 tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac tcaagcacga
     7921 ggcgaagggg ctccttaagc gcaaggcctc gaactctccc acccacttcc aacccgaagc
     7981 tcgggatcaa gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac gcaagggcca
     8041 taacccgtaa agaggccagg cccgcgggaa ccacacacgg cacttacctg tgttctggcg
     8101 gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc
     8161 ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc
     8221 caccttctct aggcaccgga tcaattgccg acccctcccc ccaacttctc ggggactgtg
     8281 ggcgatgtgc gctctgccca ctgacgggca ccggagccaa ttcccactcc tttcaagacc
     8341 tagaaggtcc attagctgca aagattcctc tctgtttaaa actttatcca tctttgcacc
     8401 cgggccccct cgagccggcg ccaaagtgga tctctgctgt ccctgtaata aacccgaaaa
     8461 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
     8521 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
     8581 atgaatactg ccatttgtct caagatctag ttacgccaag cttggatcca agcaccgtct
     8641 cgcggcccaa gcttaaaaaa acgagacctc cctatcagtg atagagattg acatccctat
     8701 cagtgataga gatactgagc acggatctga aagaggagaa aggatctatg gcgagtagcg
     8761 aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc
     8821 acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta
     8881 aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt
     8941 tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac
     9001 tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg
     9061 ttaccgttac ccaggactcc tccctgcaag acggtgagtt c
//
