LOCUS       sequence-435617-        8504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing epegRNA to induce GBA N370S mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8504)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 8504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: lenti-GBA-N370S-tmpknot-epegRNA
FEATURES             Location/Qualifiers
     source          1..8504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    114..347
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    840..965
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1012..1192
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1193..1419)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1447..1467)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1447..1465)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1486..1502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1486..1502)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1499..1521)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1510..1526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1534..1540,1541..1558,1559..1564))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1579..1600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1717..1734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1888..2476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1968..1987)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2647..3438,3439..3507))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3270..3289
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3508..3612)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3638..4093)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3775..3796)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3987..4006
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4220..4242
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4234..4251
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4235..4251
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4261..4280
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4261..4279
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4300..4435
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4475..4596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4486..4505)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4540..4559
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4674..4907
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4979..5567
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5085..5096
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5494..5514
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5583..6182)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5666..5686)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6163..6182
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6195..7373)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6204..7142
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6227..6247)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7512..7629
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(7866..7941)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7963..8211)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(8021..8040)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(8191..8211)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(8389..8433)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat
       61 tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct
      121 gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc
      181 agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct
      241 gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc
      301 ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc
      361 ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa
      421 tttttactac tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc
      481 caggtctgaa gatcagcggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc
      541 tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag
      601 agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt
      661 tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact
      721 gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt
      781 ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg
      841 acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc
      901 accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga
      961 gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga
     1021 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     1081 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     1141 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccgtttatgt
     1201 atcgagctag gcacttaaat acaatatctc tgcaatgcgg caattcagtg gttcgtccaa
     1261 tccatgtcag acccgtctgt tgccttccta ataaggcacg atcgtaccac cttacttcca
     1321 ccaatcggca tgcacggtgc tttttctctc cttgtaaggc atgttgctaa ctcatcgtta
     1381 ccatgttgca agactacaag agtattgcat aagactacat taagcttgca gctccagctt
     1441 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     1501 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1561 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1621 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1681 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1741 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1801 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1861 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1921 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1981 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2041 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2101 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2161 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2221 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2281 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2341 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2401 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2461 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2521 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2581 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2641 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2701 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2761 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2821 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2881 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2941 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3001 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3061 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3121 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3181 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3241 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3301 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3361 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3421 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3481 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3541 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3601 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     3661 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     3721 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     3781 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3841 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3901 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3961 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     4021 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     4081 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     4141 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     4201 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
     4261 taatacgact cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc
     4321 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     4381 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     4441 gagttagggg cgggatagct agagccagac atgataagat acattgatga gtttggacaa
     4501 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     4561 ttatttgtaa ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt
     4621 ctttgcaagt tataaatact gaataataag atgacatgaa ctactatacg tactgctaga
     4681 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     4741 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     4801 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     4861 agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc cccccttttc
     4921 ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta aagtcgacgc
     4981 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     5041 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     5101 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga
     5161 aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     5221 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     5281 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     5341 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     5401 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     5461 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     5521 agaataccag tcaatctttc acaaattttg taatccagag gttgattgtc gacttaacgc
     5581 gttcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     5641 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
     5701 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
     5761 ctgcccagac ccttgccctg gtggtcgggc gagactccga cggtggccag gaaccacgcg
     5821 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     5881 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     5941 ctctccggcg tggtccagac cgccacggcg gcgccgtcgt ccgcgaccca caccttgccg
     6001 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     6061 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     6121 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     6181 atggtggccg tacgtcacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga
     6241 ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc
     6301 aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca
     6361 taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg
     6421 tgcctggacg gcgcccggta ctccgtggag tcacatgaag cgacggctga ggacggaaag
     6481 gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat
     6541 tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc
     6601 gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc
     6661 agagcaggcc ggccagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg
     6721 ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca
     6781 aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa
     6841 aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg
     6901 agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc aggccaggcc
     6961 tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact ctcccaccca
     7021 cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga agtaattcaa
     7081 ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt
     7141 acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
     7201 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
     7261 cagtttaccc cgcgccacct tctctaggca ccggatcaat tgccgacccc tccccccaac
     7321 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gccaattccc
     7381 actcctttca agacctagaa ggtccattag ctgcaaagat tcctctctgt ttaaaacttt
     7441 atccatcttt gcacccgggc cccctcgagc cggcgccaaa gtggatctct gctgtccctg
     7501 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
     7561 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     7621 cttttaaaat tgtggatgaa tactgccatt tgtctcaaga tctagttacg ccaagcttgg
     7681 atccaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7741 catgtcatat gtagagaggt acctcgagcg gcccaagctt aaaaaaagcc ccccgacttt
     7801 gagggttatc ctgggttcag ggggggggct cctgagacca catggtacag gaggctctag
     7861 ggtaaggacc gactcggtcc cactttttca agttgataac ggactagcct tattttaact
     7921 tgctatttct agctctaaaa ccctgtacca tgtggtcggc tcggtgtttc gtcctttcca
     7981 caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc
     8041 cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca
     8101 cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta tctctctaac
     8161 agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct ctctgggtcc
     8221 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     8281 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     8341 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     8401 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     8461 taaggagtgt attaagcttg tgtaattgtt aatttctctg tccc
//
