LOCUS       sequence-435645-        9662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses DN-REST in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Cassady JP, D'Alessio AC, Sarkar S, Dani VS, Fan ZP, Ganz K, 
            Roessler R, Sur M, Young RA, Jaenisch R
  TITLE     Direct Lineage Conversion of Adult Mouse Liver Cells and B 
            Lymphocytes to Neural Stem Cells.
  JOURNAL   Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. doi: 
            10.1016/j.stemcr.2014.10.001.
  PUBMED    25454632
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. doi: 
            10.1016/j.stemcr.2014.10.001."
COMMENT     Sequence Label: TetO-FUW-DN-REST
FEATURES             Location/Qualifiers
     source          1..9662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1780..1798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1822..1840
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1864..1882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1915..1953
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1950..1974
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2059..2068
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3520..4108
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3573..3593)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3991..4002)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4111..4127)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4112..4128)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4633..4813
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4839..4856)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4845..5069
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5115..5543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5202..5221)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5412..5433
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5552..5572)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5557..5886
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5737..5872
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5799..5818
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5934..5981
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6000..6374
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6504..6625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6541..6560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6595..6614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6674..6690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6674..6690)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6687..6709)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6698..6714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6722..6728,6729..6746,6747..6752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6767..6788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7076..7664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7156..7175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7835..8626,8627..8695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8458..8477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8696..8800)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8875..8894)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9066..9445
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9447..9645
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt tagatctcga gtttaccact ccctatcagt gatagagaaa
     1801 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     1861 cactccctat cagtgataga gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg
     1921 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     1981 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc
     2041 ggccccgaat tcgcccttgc caccatgggc agaactgatg ccggttgggg ataacaactt
     2101 ttcagatagt gaagaaggag aaggacttga agagtctgct gatataaaag gtgaacctca
     2161 tggactggaa aacatggaac tgagaagttt ggaactcagc gtcgtagaac ctcagcctgt
     2221 atttgaggca tcaggtgctc cagatattta cagttcaaat aaagatcttc cccctgaaac
     2281 acctggagcg gaggacaaag gcaagagctc gaagaccaaa ccctttcgct gtaagccatg
     2341 ccaatatgaa gcagaatctg aagaacagtt tgtgcatcac atcagagttc acagtgctaa
     2401 gaaatttttt gtggaagaga gtgcagagaa gcaggcaaaa gccagggaat ctggctcttc
     2461 cactgcagaa gagggagatt tctccaaggg ccccattcgc tgtgaccgct gcggctacaa
     2521 tactaatcga tatgatcact atacagcaca cctgaaacac cacaccagag ctggggataa
     2581 tgagcgagtc tacaagtgta tcatttgcac atacacaaca gtgagcgagt atcactggag
     2641 gaaacattta agaaaccatt ttccaaggaa agtatacaca tgtggaaaat gcaactattt
     2701 ttcagacaga aaaaacaatt atgttcagca tgttagaact catacaggag aacgcccata
     2761 taaatgtgaa ctttgtcctt actcaagttc tcagaagact catctaacta gacatatgcg
     2821 tactcattca ggtgagaagc catttaaatg tgatcagtgc agttatgtgg cctctaatca
     2881 acatgaagta acccgccatg caagacaggt tcacaatggg cctaaacctc ttaattgccc
     2941 acactgtgat tacaaaacag cagatagaag caacttcaaa aaacatgtag agctacatgt
     3001 gaacccacgg cagttcaatt gccctgtatg tgactatgca gcttccaaga agtgtaatct
     3061 acagtatcac ttcaaatcta agcatcctac ttgtcctaat aaaacaatgg atgtctcaaa
     3121 agtgaaacta aagaaaacca aaaaacgaga ggctgacttg cctgataata ttaccaatga
     3181 aaaaacagaa atagaacaaa caaaaataaa aggggatgtg gctggaaaga aaaatgaaaa
     3241 gtccgtcaaa gcagagaaaa gagatgtctc aaaagagaaa aagccttcta ataatgtgtc
     3301 agtgatccag gtgactacca gaactcgaaa atcagtaaca gaggtgaaag agatggatgt
     3361 gcatacagga agcaattcag aaaaattcag taaaactaag aaaagcaaaa ggaagctgga
     3421 agttgacagc cattctttac atggtcctgt gaatgatgag gaatcttcaa caaaaaagaa
     3481 aaagaataaa agggcgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     3541 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3601 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3661 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3721 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3781 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3841 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3901 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3961 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4021 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4081 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
     4141 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     4201 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     4261 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     4321 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     4381 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     4441 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     4501 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     4561 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     4621 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4681 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4741 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4801 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     4861 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     4921 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     4981 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     5041 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     5101 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5161 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5221 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5281 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5341 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5401 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5461 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5521 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     5581 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5641 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5701 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5761 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5821 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5881 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     5941 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     6001 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     6061 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     6121 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     6181 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     6241 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     6301 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     6361 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     6421 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6481 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     6541 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6601 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     6661 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6721 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6781 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6841 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6901 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6961 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7021 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7081 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7141 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7201 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7261 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7321 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7381 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7441 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7501 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7561 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7621 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7681 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7741 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7801 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7861 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7921 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7981 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8041 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8101 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8161 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8221 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8281 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8341 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8401 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8461 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8521 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8581 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8641 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8701 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8761 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8821 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     8881 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     8941 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     9001 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     9061 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     9121 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     9181 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     9241 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     9301 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     9361 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     9421 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     9481 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     9541 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     9601 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
     9661 ct
//
