LOCUS       sequence-435658-        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0, N-terminal tag module (CCAT-AATG) containing 
            LoxP-mCherry-stop..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Chiang BJ, Lin KY, Chen YF, Huang CY, Goh FJ, Huang LT, Chen LH, Wu 
            CH
  TITLE     Development of a tightly regulated copper-inducible transient gene 
            expression system in Nicotiana benthamiana incorporating suicide 
            exon and Cre recombinase
  JOURNAL   bioRxiv 2024.03.23.586378
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.03.23.586378"
COMMENT     Sequence Label: pCHW110035
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(18..51)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(278..296)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    905..1079
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    complement(1089..1122)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtctctcca tgacctaata acttcgtata gcatacatta tacgaagtta tattaagggt
       61 tgaatgaaaa ctaatctttt tctctttctc atcttttcac ttctcctatc attatcctcg
      121 gccgaattcg gaggtgtgag caagggcgag gaggataaca tggccatcat caaggagttc
      181 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      241 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      301 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      361 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagtaatag
      421 tgaggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      481 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      541 ttcccctccg acggccccgt aatgcagaag aaaaccatgg gctgggaggc ctcctccgag
      601 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
      661 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      721 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      781 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      841 ctgtacaagg gtggccatga tgagctttaa gcttctctag ctagagtcga tcgacaagct
      901 cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag
      961 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     1021 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcg
     1081 ctgacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg ttgaatgtga
     1141 gacc
//
