LOCUS       sequence-435680-        5879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive GFP-expression vector for Fusobacterium nucleatum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ponath F, Zhu Y, Cosi V, Vogel J
  TITLE     Expanding the genetic toolkit helps dissect a global stress response
            in the early-branching species Fusobacterium nucleatum.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2201460119. doi: 
            10.1073/pnas.2201460119. Epub 2022 Sep 26.
  PUBMED    36161895
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-4"; volume: "119(40):e2201460119. 
            doi"; pages: " 10.1073/pnas.2201460119. Epub 2022 Sep 26"
COMMENT     Sequence Label: pVoPo-GFP
FEATURES             Location/Qualifiers
     source          1..5879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      360..394
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             492..1205
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     rep_origin      1428..2016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1917..1936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             4125..4133
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(5649..5660)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ttcaatgcta cttaattctt caaaatctct tctttttctt cctggtcttc ttttaaagtc
       61 aaaagcagtt aaagcatcaa tattgaaaat gtattcagtt atattttttg attttatttc
      121 tttagtgtta taaaatttca actcaataaa ttcccccttt attaatctgc ataatctttt
      181 ttataatgtc ctttacaacc taatattctt aatctatcat tttttaagtc ataaattagt
      241 ctatgttcat cacttattct tctgctataa atagccctat ttttaaaatg ttttaatctc
      301 tcaggcttac cagtaccgcg gccgctccag taatgacctc agaactccat ctggatttgt
      361 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc actagtaagc
      421 ataaaaaaaa gttggataat atactaaaat agtgtataat aaatatgtat attagaaagg
      481 aggaatatat aatgagaaaa ggagaagaac tttttacagg tgttgtacca atattagttg
      541 aattagatgg agatgttaat ggtcataaat tttcagttag aggagaagga gaaggtgatg
      601 caactaatgg aaaattaact cttaagttta tatgtacaac aggaaaatta cctgtaccat
      661 ggcctacttt agtaactaca ctaacatatg gagttcaatg ttttgctaga tatccagatc
      721 atatgaaaca acatgacttt ttcaaatctg caatgccaga aggatatgtt caagaaagaa
      781 caataagttt taaagatgat ggtacatata aaactagagc agaagttaaa tttgaaggag
      841 atactttagt taatagaata gagttaaaag gaattgactt taaagaagat ggaaatattt
      901 taggacataa acttgagtat aactttaatt ctcataatgt ttacattact gctgataaac
      961 aaaagaatgg tataaaagct aatttcaaga taagacataa tgtagaagat ggttctgtac
     1021 aattagcaga tcattatcag caaaatacac ctattggaga tggtcctgta ttattaccag
     1081 ataatcacta tctttcaact caatcagtac taagtaaaga tcctaatgaa aaaagagatc
     1141 atatggtttt gttagaattt gttacagctg caggaattac tcatggaatg gatgaattgt
     1201 ataaataagg atcctataag ttttaataaa actttaaata gaaaaaggct tctctaatga
     1261 gaagtctttt ttatttaaaa taaatataaa ataaaataga ggctataaat agcctctatt
     1321 ttatgtgaga aatccctaaa taaaaaacta gtcatgacca aaatccctta acgtgagttt
     1381 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1441 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1501 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1561 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1621 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1681 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1741 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1801 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1861 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1921 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1981 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc gctgagagat
     2041 cccctcataa tttccccaaa gcgtaaccat gtgtgaataa attttgagct agtagggttg
     2101 cagccacgag taagtcttcc cttgttattg tgtagccaga atgccgcaaa acttccatgc
     2161 ctaagcgaac tgttgagagt acgtttcgat ttctgactgt gttagcctgg aagtgcttgt
     2221 cccaaccttg tttctgagca tgaacgcccg caagccaaca tgttagttga agcatcaggg
     2281 cgattagcag catgatatca aaacgctctg agctgctcgt tcggctatgg cgtaggccta
     2341 gtccgtaggc aggacttttc aagtctcgga aggtttcttc aatctgcatt cgcttcgaat
     2401 agatattaac aagttgtttg ggtgttcgaa tttcaacagg taagttagtt gctagaaccc
     2461 atggctcctt tgccgacgct gagtagattt taggtgacgg gtggtgacaa tgagtccgtg
     2521 tcgagcgctg attttttcgg cctttagagc gagatttata caatagaatt tggcatgaga
     2581 ttggattgct tttagtcagc ctcttatagc ctaaagtctt tgagtgacta gatgacatat
     2641 catgtaagtt gctgataggt ttccagtttt ccgctcctag gtctgcatat tgtacttttc
     2701 ctcttactcg acttaaccag taccaaccca gcttctcaac ggatttatac catggcactt
     2761 taaagccagc atcactgaca atgagcggtg tggtgttact cggtagaatg ctcgcaaggt
     2821 cggctagaaa ttggtcatga gctttctttg aacattgctc tgaaagcggg aacgctttct
     2881 cataaagagt aacagaacga ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt
     2941 tttgctcacg aatatcagac cagtcaacaa gtacaatggg catcgtattg cccgaacaga
     3001 taaagctagc atgccaacgg tatacagcga gtcgctcttt gtggaggtga cgattaccta
     3061 acaatcggtc gattcgtttg atgttatgtt ttgttctcgc tttggttggc aggttacggc
     3121 caagttcggt aagagtgaga gttttacagt caagtaatgc gtggcaagcc aacgttaagc
     3181 tgttgagtcg ttttaagtgt aattcggggc agaattggta aagagagtcg tgtaaaatat
     3241 cgagttcgca catcttgttg tctgattatt gatttttcgc gaaaccattt gatcatatga
     3301 caagatgtgt atccacctta acttaatgat ttttaccaaa atcattaggg gattcatcag
     3361 agcaacgcgg cctttttacg gttcctggcc ttttgctggc gaattcttat ggattttagg
     3421 gtaacaaaaa acaccgtatt tctacgatgt ttttgcttaa atacttgttt ttagttacag
     3481 acaaacctga agttaactat ttatcaattc ctgcaattcg tttacaaaac ggcaaatgtg
     3541 aaatccgtca catactgcgt gatgaacttg aattgccaaa ggaagtataa ttttgttatc
     3601 ttctttataa tatttcccca tagtaaaaat aggaatcaaa taatcatatc ctttctgcaa
     3661 attcagatta aagccatcga aggttgacca cggtatcata gatacattaa aaatgttttc
     3721 cggagcattt ggctttcctt ccattctatg attgtttcca taccgttgcg tatcactttc
     3781 ataatctgct aaaaatgatt taaagtcaga cttacactca gtccaaaggc tggaaaatgt
     3841 ttcagtatca ttgtgaaata ttgtatagct tggtatcatc tcatcatata tccccaattc
     3901 accatcttga ttgattgccg tcctaaactc tgaatggcgg tttacaatca ttgcaatata
     3961 ataaagcatt gcaggatata gtttcattcc cttttccttt atttgtgtga tatccacttt
     4021 aacggtcatg ctgtaggtac aaggtacact tgcaaagtag tggtcaaaat actcttttct
     4081 gttccaacta tttttatcaa ttttttcaaa taccatttaa aattacctcc ttaaaatata
     4141 ctgctttaat tgtataattt tagctaatta ttgtcaagta tttttaaagt tatgtaaatt
     4201 ttttacttat attttatcga tcgttctaga tattcttttt taaacttttc aaatgcttca
     4261 aattctacat ctgcaacttc tacatctttt ccagtatttc caaacttcac acaatttttg
     4321 taaacatttc ttaatttttc caagtattct atgtcttcct tttcttcttt aacatactct
     4381 tgtaactgtg tcatcaaatt agttttgtct tccacattcc ccctttttct tgtaattgcc
     4441 ttatttaatt cttcaaatct tattgttttc attttattgt acctccaatt tttttatata
     4501 tctatgttaa aaaacatatt gacaattgat taaaacaatg atattatggt cggttgagct
     4561 cattgagctg agtgcagact ttcccccttg taaaaagggg aaaaaacgat tgataatgat
     4621 acaatggtcg tatatcgcta tgttcgcatc gtttaactta ctttatcgca tatttaatta
     4681 tatgccagtg ttagccctgc ccaacaccta gagttttagc ttactttcta aattataacc
     4741 ctaataaagt acccactcag agcacctcca agacttttaa aggtagcctc gcaccttgct
     4801 acaattcata ataccaacat tacggtgcta taccgtttcg agcagaacgg ctatacatac
     4861 gaatttatct tatgtataaa atttgttaca aatataatag taagtttttt ttatataaaa
     4921 aagcagatcg cttttatttt ttttgcaacc tgcttcacaa ctcatttttt tactttagta
     4981 aataaatcct ttacatcttt taaaaaatat ggtatattaa gtcaaagcag gcataacata
     5041 tatgttatgt tttatagtaa aaatggacta ttcaactatt taaaaaatgg tagaatacaa
     5101 ataattttcc tggcagtcaa ttatttttta aaaattttta ctattatatt tttaaatttt
     5161 aaattttaga tagagttatt tggcgataac tctttttgtc ttacttctga gtttagcata
     5221 ttttttttgt tttgtaaatc catttcttta tttttcttta caacataaac tttttttact
     5281 tctctataaa tcttctcacc agttgaaaca tcaatttcta acagttctct agcaataaaa
     5341 tttttaatat agagtaaatc tttttcatct gtaagtatat cattcatttc atcaaaatcc
     5401 aacttttttt tggtttcttt tggttttatt aaatttcgtg aacagaaata ggcttttttt
     5461 tgatttgctt tcttttctaa gtctttcaca aaatacttcg ttatatacac ccccacagct
     5521 tcatttttaa cagtttcttt aatcttattt atttttataa atccattcgc ccacaattcc
     5581 ccaagcttct tattatctat atatgggacc gagaacagca ccaaatgata atggattgct
     5641 cccctctgct ggagttccca agttgcaata tatttaagct gttgattttt caagtacctt
     5701 ttcaatctct taataaataa agtaaattca cgatttgccc tttctatatc ctgtatattt
     5761 tctttaaaag taagagttaa aaaggtactt ttatcatcat aattacaatc aattatcctt
     5821 tttatttcaa atcgcttgtc ctggtaatat gctttccttc gttgcatact ttcagcttt
//
