LOCUS       sequence-435687-        9417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of 
            CLIPf-KIF5A(Delta-954-1027)-SNAPf-HisTag-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Smith ER, Turner ED, Abdelhamid MAS, Craggs TD, Twelvetrees AE
  TITLE     Kinesin-1 is highly flexible and adopts an open conformation in the 
            absence of cargo
  JOURNAL   bioRxiv 2024.12.20.629623
REFERENCE   2  (bases 1 to 9417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.12.20.629623"
COMMENT     Sequence Label: CLIPf-KIF5A(Delta-954-1027)-SNAPf-HisTag-FLAG
FEATURES             Location/Qualifiers
     source          1..9417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1875..1892
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1893..1916
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(1964..1981)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1970..2194
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2240..2668
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2327..2346)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2537..2558
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2677..2697)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2682..3011
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2862..2997
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2924..2943
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3078..3872
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3132..3151)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3742..3761
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4046..4167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4083..4102)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4137..4156
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4216..4232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4216..4232)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4229..4251)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4240..4256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4264..4270,4271..4288,4289..4294))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4309..4330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4447..4464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4618..5206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4698..4717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5377..6168,6169..6237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6000..6019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6238..6342)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6417..6436)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6608..6987
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6988..7191
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7142..7162
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7236..7255
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7236..7254
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccaccatgct gtccctggag tccgagctac agcggctcca ggaggtcagt ggacaccagc
       61 gaaagcggat cgctgaggtg ctgaatgggc tgatgaggga cctgagtgag ttcagtgtca
      121 tcgtgggcaa cggcgagatt aagctgcccg tggagatcag tggggccatc gaggaggagt
      181 tcacggtggc ccggctctac atcagcaaga tcaagtcgga ggtgaagtcc gtggttaagc
      241 gatgtcggca gctggagaac ctccaggtgg agtgtcatcg caagatggag gtgaccggta
      301 gggagctgtc atcttgccaa ctgctcatct cacagcatga ggccaagatc cgttcactca
      361 cggagtacat gcagactgtg gagttgaaga aacggcacct ggaagagtcc tacgactccc
      421 tgagcgatga gcttgccagg ctccaggcgc acgaaactgt acacgaggta gctctgaaag
      481 acaaggagcc agacacacag gacgcggagg aggtgaagaa ggccctggaa ctacagatgg
      541 agaatcaccg tgaggcccat caccggcagc tggcccgcct ccgggatgag attaatgaga
      601 aacagaaaac cattgatgag ctgaaagacc tgaaccagaa gctccagtta gagctggaga
      661 agcttcaggc cgactatgag aggctgaaga atgaagagaa cgagaagagc gccaagctcc
      721 aggagctgac atttctgtat gagcgacatg agcagtccaa gcaggacctc aaggggctgg
      781 aggagacagt tgcccgtgaa ctccagaccc tccacaacct tcgcaagctg ttcgttcaag
      841 acgtcacgac tcgagtcaag aaaagtgcag aaatggagcc cgaggacagt ggggggattc
      901 attcccaaaa gcagaagatc tcctttcttg agaacaacct ggaacagctt acaaaggttc
      961 acaaacagct ggtacgtgac aatgcagatc tgcgttgtga gcttcctaaa ttggaaaaac
     1021 gacttcgggc tacggctgag agagttaagg ccctggaggg tgcactgaag gaggccaagg
     1081 agggcgctat gaaggacaag cgtagatacc agcaggaggt ggaccgcatc aaagaagccg
     1141 tgcggtacaa gagctccggc aagcggggcc attctgccca gatcgctaag cctgtgaggc
     1201 ctggccacta tcctgcctcc tcacccacca acccctacgg cacccggagc cccgagtgta
     1261 tcagctacac caacaacctc ttccagaacg aattcgataa ggattgtgaa atgaagcgca
     1321 caaccctgga ctccccactg ggcaaacttg agctgtctgg atgcgagcag ggactgcaca
     1381 gaatcatatt cctcggaaag ggcaccagcg ccgctgatgc agtggaagtt ccagcaccag
     1441 ctgcagtgct tggtggacca gagccactta tgcaggctac agcttggctg aatgcttatt
     1501 tccaccagcc ggaagccatc gaagagtttc ccgtgccagc actgcaccat cctgtctttc
     1561 aacaagagtc cttcacacgg caagtcctgt ggaaactcct gaaagtcgtg aaatttgggg
     1621 aagtgatctc ctacagccac ctggctgcac tggccggaaa tcctgcagca actgccgctg
     1681 tgaaaactgc cctgtccggc aatccagtgc caatcctgat tccttgtcac cgggtcgtcc
     1741 agggcgacct ggatgttgga ggatatgaag gcggactggc tgtcaaagag tggctccttg
     1801 ctcacgaggg ccatagactc ggaaagcctg gacttggtgg aagcgccgga tctgcagcag
     1861 gatctggcga gtttcaccac caccatcacc acgactacaa ggacgacgac gacaaatgag
     1921 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     1981 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2041 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2101 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2161 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     2221 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2281 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2341 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2401 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2461 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2521 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     2581 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     2641 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     2701 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2761 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2821 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     2881 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     2941 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3001 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     3061 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     3121 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3181 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3241 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3301 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3361 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3421 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3481 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3541 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3601 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3661 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3721 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3781 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3841 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3901 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3961 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4021 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     4081 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4141 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     4201 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4261 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4321 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4381 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4441 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4501 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4561 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4621 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4681 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4741 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4801 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4861 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4921 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4981 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5041 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5101 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5161 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5221 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5281 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5341 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5401 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5461 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5521 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5581 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5641 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5701 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5761 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5821 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5881 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5941 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6001 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6061 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6121 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6181 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6241 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6301 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6361 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     6421 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     6481 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     6541 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     6601 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     6661 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     6721 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     6781 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     6841 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     6901 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     6961 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     7021 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     7081 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     7141 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     7201 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
     7261 caagctggct agcgtttaaa cttaagcttg gtaccatgga caaggactgc gagatgaagc
     7321 ggaccacact ggatagccct ctgggcaagc tggaactgag cggctgtgaa cagggcctgc
     7381 accggatcat ctttctcggc aagggaacct ctgccgccga tgctgttgaa gtgcctgctc
     7441 ctgctgctgt gctcggagga cctgaacctc tgattcaggc caccgcctgg ctgaacgcct
     7501 actttcatca gcccgaggcc atcgaggagt tccccgttcc tgctctgcat caccccgtgt
     7561 tccagcaaga aagcttcacc agacaggtgc tgtggaagct gctgaaggtg gtcaagttcg
     7621 gcgaagtgat cagcgagtct cacctggccg ctctcgtggg aaatccagct gctacagccg
     7681 ccgtgaacac agccctggat ggaaaccctg tgcctattct gatcccctgc cacagagtgg
     7741 tgcagggcga ttctgatgtg ggcccttatc ttggaggcct ggccgtgaaa gaatggctgc
     7801 tggcccacga aggacacaga ctgggaaaac ctggcctcgg aggatccatg gcggagacta
     7861 acaacgaatg cagcatcaag gtgctttgcc gatttcggcc cctgaaccag gccgagattc
     7921 tgcgggggga caagttcatc cccattttcc aaggggacga cagcgtcatt attgggggaa
     7981 agccatatgt ctttgaccgc gtcttccccc caaacaccac tcaggagcag gtttaccacg
     8041 cctgtgccat gcagatcgtc aaagacgtcc ttgctggtta caatggcaca atcttcgctt
     8101 atggacagac atcctcaggg aaaacgcata ccatggaggg gaagctgcac gaccctcagc
     8161 tgatgggcat cattccccgg atcgctcgag acatcttcaa ccacatctac tccatggatg
     8221 agaaccttga attccacatt aaggtatctt acttcgagat ttacctggat aagatccgtg
     8281 accttttgga tgtgaccaag acgaacctgt ccgtgcatga ggacaaaaac cgggtgccgt
     8341 ttgtcaaggg ttgtaccgaa cgctttgtgt ccagcccaga ggagattctg gatgtgatcg
     8401 atgaggggaa gtccaaccgt cacgtagctg tcaccaacat gaacgagcac agttctcgga
     8461 gccacagcat cttcctcatc aacatcaagc aggagaacgt agagaccgag cagaagctca
     8521 gcgggaagct gtacctcgtg gatctggccg gaagcgagaa ggtcagcaag acaggggcag
     8581 agggagccgt tctggacgag gcaaagaata tcaacaagtc gctgtcggcc ctggggaacg
     8641 tgatctctgc actggcagag ggcaccaaaa gctacgtgcc gtaccgcgac agcaaaatga
     8701 cgaggattct ccaggactct ctgggaggga actgcaggac taccatgttc atctgctgct
     8761 cgccgtccag ctacaatgac gcagagacca agtccacgct catgtttgga cagcgggcga
     8821 agaccatcaa gaacactgcc tcagtgaatc tggagctgac tgctgagcag tggaagaaga
     8881 agtatgagaa ggagaaggag aagaccaagg cccagaagga gacaattgcg aagctagagg
     8941 ctgagcttag ccggtggcgc aatggagaga atgtgcctga gactgagcgc ctggctggag
     9001 aggactcagc tctgggagct gagctctgcg aggagacccc tgtgaatgac aactcatcca
     9061 ttgtggtacg catcgcacct gaggaaaggc agaaatatga ggaagagatc cgccgtctct
     9121 acaagcagct tgatgacaag gatgatgaga tcaaccagca gagccagctc attgagaagc
     9181 tgaagcagca gatgctggac caggaagagc tgctcgtgtc cactcgggga gacaacgaga
     9241 aggtccagcg ggagcttagc cacctgcagt ccgagaacga tgctgcgaag gacgaggtga
     9301 aggaagtgct gcaggcccta gaggagctgg cggtcaacta cgaccagaag tcccaggagg
     9361 tggaggagaa gagccagcag aaccagctgc tggtggacga gctgtcccag aaagtgg
//
