LOCUS       sequence-435693-        9450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of CLIPf-KIF5B-SNAPf-HisTag-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Smith ER, Turner ED, Abdelhamid MAS, Craggs TD, Twelvetrees AE
  TITLE     Kinesin-1 is highly flexible and adopts an open conformation in the 
            absence of cargo
  JOURNAL   bioRxiv 2024.12.20.629623
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.12.20.629623"
COMMENT     Sequence Label: CLIPf-KIF5B-SNAPf-HisTag-FLAG
FEATURES             Location/Qualifiers
     source          1..9450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        25..228
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     179..199
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     273..292
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        273..291
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4362..4379
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4380..4403
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4451..4468)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4457..4681
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4727..5155
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4814..4833)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5024..5045
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5164..5184)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5169..5498
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5349..5484
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5411..5430
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5565..6359
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5619..5638)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6229..6248
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6533..6654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6570..6589)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6624..6643
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6703..6719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6703..6719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6716..6738)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6727..6743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6751..6757,6758..6775,6776..6781))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6796..6817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6934..6951)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7105..7693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7185..7204)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7864..8655,8656..8724))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8487..8506
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8725..8829)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8904..8923)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9095..9450,1..24)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
       61 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      121 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      181 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      241 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      301 gctggctagc gtttaaactt aagcttggta ccatggacaa ggactgcgag atgaagcgga
      361 ccacactgga tagccctctg ggcaagctgg aactgagcgg ctgtgaacag ggcctgcacc
      421 ggatcatctt tctcggcaag ggaacctctg ccgccgatgc tgttgaagtg cctgctcctg
      481 ctgctgtgct cggaggacct gaacctctga ttcaggccac cgcctggctg aacgcctact
      541 ttcatcagcc cgaggccatc gaggagttcc ccgttcctgc tctgcatcac cccgtgttcc
      601 agcaagaaag cttcaccaga caggtgctgt ggaagctgct gaaggtggtc aagttcggcg
      661 aagtgatcag cgagtctcac ctggccgctc tcgtgggaaa tccagctgct acagccgccg
      721 tgaacacagc cctggatgga aaccctgtgc ctattctgat cccctgccac agagtggtgc
      781 agggcgattc tgatgtgggc ccttatcttg gaggcctggc cgtgaaagaa tggctgctgg
      841 cccacgaagg acacagactg ggaaaacctg gcctcggagg atccatggcg gacctggccg
      901 agtgcaacat caaagtgatg tgtcgcttca gacctctcaa cgagtctgaa gtgaaccgcg
      961 gcgacaagta catcgccaag tttcagggag aagacacggt cgtgatcgcg tccaagcctt
     1021 atgcatttga tcgggtgttc cagtcaagca catctcaaga gcaagtgtat aatgactgtg
     1081 caaagaagat tgttaaagat gtacttgaag gatataatgg aacaatattt gcatatggac
     1141 aaacatcctc tgggaagaca cacacaatgg agggtaaact tcatgatcca gaaggcatgg
     1201 gaattattcc aagaatagtg caagatattt ttaattatat ttactccatg gatgaaaatt
     1261 tggaatttca tattaaggtt tcatattttg aaatatattt ggataagata agggacctgt
     1321 tagatgtttc aaagaccaac ctttcagttc atgaagacaa aaaccgagtt ccctatgtaa
     1381 aggggtgcac agagcgtttt gtatgtagtc cagatgaagt tatggatacc atagatgaag
     1441 gaaaatccaa cagacatgta gcagttacaa atatgaatga acatagctct aggagtcaca
     1501 gtatatttct tattaatgtc aaacaagaga acacacaaac ggaacaaaag ctgagtggaa
     1561 aactttatct ggttgattta gctggtagtg aaaaggttag taaaactgga gctgaaggtg
     1621 ctgtgctgga tgaagctaaa aacatcaaca agtcactttc tgctcttgga aatgttattt
     1681 ctgctttggc tgagggtagt acatatgttc catatcgaga tagtaaaatg acaagaatcc
     1741 ttcaagattc attaggtggc aactgtagaa ccactattgt aatttgctgc tctccatcat
     1801 catacaatga gtctgaaaca aaatctacac tcttatttgg ccaaagggcc aaaacaatta
     1861 agaacacagt ttgtgtcaat gtggagttaa ctgcagaaca gtggaaaaag aagtatgaaa
     1921 aagaaaaaga aaaaaataag atcctgcgga acactattca gtggcttgaa aatgagctca
     1981 acagatggcg taatggggag acggtgccta ttgatgaaca gtttgacaaa gagaaagcca
     2041 acttggaagc tttcacagtg gataaagata ttactcttac caatgataaa ccagcaaccg
     2101 caattggagt tataggaaat tttactgatg ctgaaagaag aaagtgtgaa gaagaaattg
     2161 ctaaattata caaacagctt gatgacaagg atgaagaaat taaccagcaa agtcaactgg
     2221 tagagaaact gaagacgcaa atgttggatc aggaggagct tttggcatct accagaaggg
     2281 atcaagacaa tatgcaagct gagctgaatc gccttcaagc agaaaatgat gcctctaaag
     2341 aagaagtgaa agaagtttta caggccctag aagaacttgc tgtcaattat gatcagaagt
     2401 ctcaggaagt tgaagacaaa actaaggaat atgaattgct tagtgatgaa ttgaatcaga
     2461 aatcggcaac tttagcgagt atagatgctg agcttcagaa acttaaggaa atgaccaacc
     2521 accagaaaaa acgagcagct gagatgatgg catctttact aaaagacctt gcagaaatag
     2581 gaattgctgt gggaaataat gatgtaaagc agcctgaggg aactggcatg atagatgaag
     2641 agttcactgt tgcaagactc tacattagca aaatgaagtc agaagtaaaa accatggtga
     2701 aacgttgcaa gcagttagaa agcacacaaa ctgagagcaa caaaaaaatg gaagaaaatg
     2761 aaaaggagtt agcagcatgt cagcttcgta tctctcaaca tgaagccaaa atcaagtcat
     2821 tgactgaata ccttcaaaat gtggaacaaa agaaaagaca gttggaggaa tctgtcgatg
     2881 ccctcagtga agaactagtc cagcttcgag cacaagagaa agtccatgaa atggaaaagg
     2941 agcacttaaa taaggttcag actgcaaatg aagttaagca agctgttgaa cagcagatcc
     3001 agagccatag agaaactcat caaaaacaga tcagtagttt gagagatgaa gtagaagcaa
     3061 aagcaaaact tattactgat cttcaagacc aaaaccagaa aatgatgtta gagcaggaac
     3121 gtctaagagt agaacatgag aagttgaaag ccacagatca ggaaaagagc agaaaactac
     3181 atgaacttac ggttatgcaa gatagacgag aacaagcaag acaagacttg aagggtttgg
     3241 aagagacagt ggcaaaagaa cttcagactt tacacaacct gcgcaaactc tttgttcagg
     3301 acctggctac aagagttaaa aagagtgctg agattgattc tgatgacacc ggaggcagcg
     3361 ctgctcagaa gcaaaaaatc tcctttcttg aaaataatct tgaacagctc actaaagtgc
     3421 acaaacagtt ggtacgtgat aatgcagatc tccgctgtga acttcctaag ttggaaaagc
     3481 gacttcgagc tacagctgag agagtgaaag ctttggaatc agcactgaaa gaagctaaag
     3541 aaaatgcatc tcgtgatcgc aaacgctatc agcaagaagt agatcgcata aaggaagcag
     3601 tcaggtcaaa gaatatggcc agaagagggc attctgcaca gattgctaaa cctattcgtc
     3661 ccgggcaaca tccagcagct tctccaactc acccaagtgc aattcgtgga ggaggtgcat
     3721 ttgttcagaa cagccagcca gtggcagtgc gaggtggagg aggcaaacaa gtgggagaat
     3781 tcgataagga ttgtgaaatg aagcgcacaa ccctggactc cccactgggc aaacttgagc
     3841 tgtctggatg cgagcaggga ctgcacagaa tcatattcct cggaaagggc accagcgccg
     3901 ctgatgcagt ggaagttcca gcaccagctg cagtgcttgg tggaccagag ccacttatgc
     3961 aggctacagc ttggctgaat gcttatttcc accagccgga agccatcgaa gagtttcccg
     4021 tgccagcact gcaccatcct gtctttcaac aagagtcctt cacacggcaa gtcctgtgga
     4081 aactcctgaa agtcgtgaaa tttggggaag tgatctccta cagccacctg gctgcactgg
     4141 ccggaaatcc tgcagcaact gccgctgtga aaactgccct gtccggcaat ccagtgccaa
     4201 tcctgattcc ttgtcaccgg gtcgtccagg gcgacctgga tgttggagga tatgaaggcg
     4261 gactggctgt caaagagtgg ctccttgctc acgagggcca tagactcgga aagcctggac
     4321 ttggtggaag cgccggatct gcagcaggat ctggcgagtt tcaccaccac catcaccacg
     4381 actacaagga cgacgacgac aaatgagcgg ccgctcgagt ctagagggcc cgtttaaacc
     4441 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4501 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4561 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4621 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     4681 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     4741 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4801 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4861 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4921 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4981 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5041 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5101 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     5161 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5221 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5281 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5341 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5401 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     5461 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     5521 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5581 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5641 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5701 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     5761 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     5821 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     5881 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     5941 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     6001 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6061 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     6121 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     6181 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     6241 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     6301 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6361 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6421 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6481 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6541 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6601 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6661 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6721 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6781 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6841 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6901 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6961 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7021 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7081 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7141 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7201 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7261 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7321 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7381 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7441 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7501 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7561 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7621 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7681 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7741 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7801 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7861 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7921 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7981 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8041 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8101 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8161 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8221 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8281 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8341 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8401 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8461 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8521 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8581 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8641 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8701 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8761 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8821 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     8881 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8941 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     9001 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     9061 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     9121 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     9181 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     9241 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     9301 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     9361 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     9421 tgggactttc ctacttggca gtacatctac
//
