LOCUS       sequence-435695-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Chiang BJ, Lin KY, Chen YF, Huang CY, Goh FJ, Huang LT, Chen LH, Wu 
            CH
  TITLE     Development of a tightly regulated copper-inducible transient gene 
            expression system in Nicotiana benthamiana incorporating suicide 
            exon and Cre recombinase
  JOURNAL   bioRxiv 2024.03.23.586378
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.03.23.586378"
COMMENT     Sequence Label: pCHW110034
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(18..51)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             136..840
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(278..296)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    896..1070
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    complement(1080..1113)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtctctcca tgacctaata acttcgtata gcatacatta tacgaagtta tattaagggt
       61 tgaatgaaaa ctaatctttt tctctttctc atcttttcac ttctcctatc attatcctcg
      121 gccgaattcg gaggtgtgag caagggcgag gaggataaca tggccatcat caaggagttc
      181 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      241 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      301 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      361 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      421 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      481 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      541 gacggccccg taatgcagaa gaaaaccatg ggctgggagg cctcctccga gcggatgtac
      601 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
      661 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      721 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
      781 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
      841 ggtggccatg atgagcttta agcttctcta gctagagtcg atcgacaagc tcgagtttct
      901 ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct
      961 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
     1021 aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc gctgacctaa
     1081 taacttcgta tagcatacat tatacgaagt tatattaagg gttgaatgtg agacc
//
