LOCUS       sequence-435710-       12508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses exogenous ribosomal-binding domain mutant NAC complex 
            members (K78E NACA and K43E BTF3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12508)
  AUTHORS   Muthukumar G, Stevens TA, Inglis AJ, Esantsi TK, Saunders RA, 
            Schulte F, Voorhees RM, Guna A, Weissman JS
  TITLE     Triaging of alpha-helical proteins to the mitochondrial outer 
            membrane by distinct chaperone machinery based on substrate 
            topology.
  JOURNAL   Mol Cell. 2024 Mar 21;84(6):1101-1119.e9. doi: 
            10.1016/j.molcel.2024.01.028. Epub 2024 Feb 29.
  PUBMED    38428433
REFERENCE   2  (bases 1 to 12508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.01.028"; journalName: "Mol Cell"; date: 
            "2024-03-21- 21"; volume: "84"; issue: "6"; pages: "1101-1119.e9"
COMMENT     Sequence Label: pGM238
FEATURES             Location/Qualifiers
     source          1..12508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             91..324
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(349..367)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(350..367)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(657..676)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(890..909)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1009..1031
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1069..1087)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1155..1259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1260..1328,1329..2120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1478..1497)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2291..2879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2780..2799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3033..3050
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3120..3140)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3125..3454
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3305..3440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4588..4653
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4783..4803
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5228..5362
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5265..5284)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5319..5338
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5530..6163
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6210..6335
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6832..7065
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7250..7294
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7579..7696
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8428..9605
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8659..9597
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9554..9574
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             9669..10367
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             10377..10433
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10446..10511
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             11232..11285
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             11292..11309
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    11901..12489
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11954..11974)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12372..12383)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12492..12508)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(12493..12508,1))
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
       61 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
      121 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
      181 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      241 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      301 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
      361 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
      421 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
      481 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
      541 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
      601 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      661 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
      721 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
      781 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
      841 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
      901 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
      961 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     1021 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     1081 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     1141 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1201 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1261 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1321 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1381 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1441 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1501 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1561 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1621 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1681 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1741 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1801 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1861 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1921 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1981 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     2041 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     2101 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     2161 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     2221 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2281 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2341 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2401 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2461 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2521 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2581 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2641 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2701 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2761 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2821 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2881 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2941 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3001 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3061 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     3121 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3181 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3301 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3361 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3421 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     3481 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     3541 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     3601 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     3661 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     3721 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     3781 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     3841 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     3901 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     3961 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     4021 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     4081 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     4141 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     4201 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     4261 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     4321 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     4381 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     4441 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     4501 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     4561 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     4621 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     4681 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     4741 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     4801 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     4861 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     4921 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     4981 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     5041 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     5101 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     5161 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     5221 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5281 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5341 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     5401 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     5461 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     5521 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     5581 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     5641 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     5701 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     5761 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     5821 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     5881 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     5941 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     6001 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6061 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6121 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     6181 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     6241 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     6301 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     6361 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     6421 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     6481 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     6541 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     6601 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     6661 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     6721 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     6781 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     6841 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     6901 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     6961 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     7021 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     7081 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     7141 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     7201 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     7261 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     7321 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     7381 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     7441 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     7501 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat
     7561 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     7621 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     7681 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa
     7741 gcttgatatc gaattcggga ggtggtccct gcagttacgc caatgataac ccccgccaga
     7801 aaaatcttag tagccttccc tttttgtttt ccgtgcccca actcggcgga ttgactcggc
     7861 cccttccgga aacacccgaa tcaacttcta gtcaaattat tgttcacgcc gcaatgaccc
     7921 acccctggcc cgcgtctgtg gaactgaccc ctggtgtaca ggagagttcg ctgctgaaag
     7981 tggtcccaaa ggggtactag tttttaagct cccaactccc cctcccccag cgtctggagg
     8041 attccacacc ctcgcaccgc aggggcgagg aagtgggcgg agtccggttt tggcgccagc
     8101 cgctgaggct gccaagcaga aaagccaccg ctgaggagac tccggtcact gtcctcgccc
     8161 cgcctccccc ttccctcccc ttggggacca ccgggcgcca cgccgcgaac ggtaagtgcc
     8221 gcggtcgtcg gcgcctccgc cctcccccta gggccccaat tcccagcggg cgcggcgcgc
     8281 ggcccctccc cccgccgggc gcgcgcccgc tgccccgccc ttcgtggccg cccggcgtgg
     8341 gcggtgccac ccctcccccc ggcggccccg cgcgcagctc ccggctccct cccccttcgg
     8401 atgtggcttg agctgtaggc gcggaggggc tccggtgccc gtcagtgggc agagcgcaca
     8461 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     8521 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     8581 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     8641 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
     8701 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
     8761 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     8821 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     8881 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     8941 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     9001 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     9061 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     9121 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     9181 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     9241 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     9301 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     9361 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     9421 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     9481 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     9541 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     9601 tcgtggaatt ctcacgcgtc tgcaggatat caagcttgcg gtaccgcggg cccgaagctt
     9661 acgccaccat gagcgagctg attaaggaga acatgcacat gaagctgtac atggagggca
     9721 ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     9781 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     9841 tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg
     9901 acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag
     9961 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
    10021 acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac
    10081 tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa
    10141 acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc aagaccacat
    10201 atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca
    10261 gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag
    10321 tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaatgga agcggagcta
    10381 ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga cctggatccg
    10441 gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg
    10501 acgatgacaa ggccggatct ggatctggca gtggatcagg atctggtagt ggtagtggta
    10561 gcggaagtgg aagtggcagt cccggcgaag ccacagaaac cgtccctgct acagagcagg
    10621 agttgccgca gccccaggct gagacagggt ctggaacaga atctgacagt gatgaatcag
    10681 taccagagct tgaagaacag gattccaccc aggcaaccac acaacaagcc cagctggcgg
    10741 cagcagctga aatcgatgaa gaaccagtca gtaaagcaaa acagagtcgg agtgaaaaga
    10801 aggcacggga ggctatgtcc aaactgggtc ttcggcaggt tacaggagtt actagagtca
    10861 ctatccggaa atctaagaat atcctctttg tcatcacaaa accagatgtc tacaagagcc
    10921 ctgcttcaga tacttacata gtttttgggg aagccaagat cgaagattta tcccagcaag
    10981 cacaactagc agctgctgag aaattcaaag ttcaaggtga agctgtctca aacattcaag
    11041 aaaacacaca gactccaact gtacaagagg agagtgaaga ggaagaggtc gatgaaacag
    11101 gtgtagaagt taaggacata gaattggtca tgtcacaagc aaatgtgtcg agagcaaagg
    11161 cagtccgagc cctgaagaac aacagtaatg atattgtaaa tgcgattatg gaattaacaa
    11221 tgggctccgg cgagggcaga ggcagtctgc tgacatgcgg tgacgtggaa gagaatcccg
    11281 gccctggatc ccatcaccac catcaccatg gcggaggtgg cagtggaggt ggcggatcag
    11341 gtggcggagg tagcaaagaa acaatcatga accaggaaaa actcgccaaa ctgcaggcac
    11401 aagtgcgcat tggtgggaaa ggaactgctc gcagaaagaa gaaggtggtt catagaacag
    11461 ccacagcaga tgacaaagag cttcagttct ccttaaagaa gttaggggta aacaatatct
    11521 ctggtattga agaggtgaat atgtttacaa accaaggaac agtgatccac tttaacaacc
    11581 ctaaagttca ggcatctctg gcagcgaaca ctttcaccat tacaggccat gctgagacga
    11641 agcagctgac agaaatgcta cccagcatct taaaccagct tggtgcggat agtctgacta
    11701 gtttaaggag actggccgaa gctctgccca aacaatctgt ggatggaaaa gcaccacttg
    11761 ctactggaga ggatgatgat gatgaagttc cagatcttgt ggagaatttt gatgaggctt
    11821 ccaagaatga ggcaaactga taaggccgcg actctagagt cgacctgcag gcatgcaagc
    11881 ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
    11941 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
    12001 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
    12061 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
    12121 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
    12181 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
    12241 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
    12301 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
    12361 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
    12421 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
    12481 cctccccgca tcgataccgt cgacctcg
//
