LOCUS       sequence-435715-        4044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC17A4_STOP
FEATURES             Location/Qualifiers
     source          1..4044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2169..2268)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2285..2304)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2286..2304)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2309..2325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2309..2325)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2427..2446)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2438..3247
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2505..2524)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3394..3982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3883..3902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgagca ccggacctga cgtgaaggca acagtgggcg acatcagctc
      721 cgatggcaac ctgaatgtgg cccaggagga gtgctccagg aagggctttt gttctgtgcg
      781 ccacggactg gccctgatcc tgcagctgtg caacttctcc atctacaccc agcagatgaa
      841 tctgtctatc gccatcccag ccatggtgaa caatacagca ccacctagcc agccaaacgc
      901 atccaccgag aggccatcca cagactctca gggctattgg aatgagaccc tgaaggagtt
      961 taaggcaatg gcaccagcat acgattggtc ccctgagatc cagggcatca tcctgtctag
     1021 cctgaactat ggctcttttc tggcccctat cccaagcggc tatgtggccg gcatcttcgg
     1081 agcaaagtac gtggtgggag caggactgtt catctcctct tttctgaccc tgttcatccc
     1141 actggcagca aatgcaggag tggccctgct gatcgtgctg aggatcgtgc agggcatcgc
     1201 ccaggtcatg gtgctgacag gccagtactc catctgggtg aagtgggcac caccactgga
     1261 gcggagccag ctgaccacaa tcgccggctc tggcagcatg ctgggaagct tcatcgtgct
     1321 gctggcagga ggactgctgt gccagaccat cggctggccc tacgtgttct atatctttgg
     1381 aggaatcgga tgtgcatgct gtccactgtg gtttcctctg atctatgacg atccagtgaa
     1441 ccaccccttc atctctgccg gcgagaagcg gtacatcgtg tgcagcctgg cccagcagga
     1501 ttgttctcct ggctggagcc tgccaatcag agccatgatc aagagcctgc ctctgtgggc
     1561 catcctggtg tcctactttt gcgagtattg gctgttctac acaatcatgg cctatacccc
     1621 cacatacatc agctccgtgc tgcaggccaa tctgagggac tccggaatcc tgtctgccct
     1681 gccttttgtg gtgggctgca tctgtatcat cctgggcggc ctgctggccg atttcctgct
     1741 gagccggaag atcctgagac tgatcaccat caggaagctg tttacagcca tcggcgtgct
     1801 gttcccttct gtgatcctgg tgagcctgcc atgggtgcgc tctagccaca gcatgaccat
     1861 gacatttctg gtgctgtcct ctgccatcag ctccttttgt gagtccggcg ccctggtgaa
     1921 cttcctggac atcgccccaa gatataccgg ctttctgaag ggcctgctgc aggtgttcgc
     1981 acacatcgca ggagcaatct ccccaacagc agcaggcttc tttatcagcc aggattccga
     2041 gtttggctgg aggaacgtgt tcctgctgag cgccgcagtg aatatctccg gcctggtgtt
     2101 ttacctgatc ttcggcagag ccgacgtgca ggattgggcc aaggagcaga ccttcacaca
     2161 cctgtaagac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2221 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     2281 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     2341 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     2401 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     2461 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     2521 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     2581 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     2641 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     2701 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     2761 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     2821 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     2881 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     2941 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     3001 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     3061 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     3121 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     3181 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     3241 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     3301 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     3361 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3421 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3481 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3541 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3601 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3661 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3721 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3781 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3841 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3901 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3961 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4021 ttgctggcct tttgctcaca tgtt
//
