LOCUS       sequence-435716-        4482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO2A1_STOP
FEATURES             Location/Qualifiers
     source          1..4482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2607..2706)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2723..2742)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2724..2742)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2747..2763)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2747..2763)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2865..2884)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2876..3685
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2943..2962)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3832..4420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4321..4340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgggcc tgctgccaaa gctgggagca tctcagggaa gcgacacctc
      721 tacaagcagg gcaggcagat gcgcacggag cgtgttcggc aacatcaagg tgttcgtgct
      781 gtgccaggga ctgctgcagc tgtgccagct gctgtacagc gcctacttca agagctccct
      841 gaccacaatc gagaagcggt tcggcctgtc tagctcctct agcggcctga tctcctctct
      901 gaacgagatc tccaatgcca tcctgatcat cttcgtgtct tactttggaa gcagggtgca
      961 caggccaaga ctgatcggaa tcggaggact gtttctggca gcaggagcct tcatcctgac
     1021 cctgccccac tttctgagcg agccttacca gtataccctg gcctctacag gcaacaatag
     1081 caggctgcag gcagagctgt gccagaagca ctggcaggat ctgccccctt ccaagtgcca
     1141 ctctaccaca cagaaccccc agaaggagac cagctccatg tggggactga tggtggtggc
     1201 acagctgctg gcaggaatcg gcacagtgcc aatccagccc tttggcatct cctacgtgga
     1261 cgatttctct gagccatcca attctcccct gtatatcagc atcctgtttg ccatctccgt
     1321 gttcggccct gcctttggct acctgctggg ctccgtgatg ctgcagatct tcgtggacta
     1381 tggccgcgtg aacaccgccg ccgtgaatct ggtgcctggc gatccaaggt ggatcggagc
     1441 atggtggctg ggactgctga tctctagcgc cctgctggtg ctgaccagct tccccttctt
     1501 tttctttcct agagccatgc caatcggagc aaagagggca cctgcaacag cagacgaggc
     1561 aaggaagctg gaggaggcaa agtcccgggg ctctctggtg gacttcatca agcggttccc
     1621 ttgtatcttc ctgcggctgc tgatgaactc cctgtttgtg ctggtggtgc tggcccagtg
     1681 caccttctcc tctgtgatcg ccggcctgag cacatttctg aataagttcc tggagaagca
     1741 gtacggcacc tccgccgcct atgccaactt tctgatcgga gcagtgaatc tgcctgcagc
     1801 cgccctggga atgctgttcg gaggaatcct gatgaagaga ttcgtgttta gcctgcaggc
     1861 catcccaagg atcgccacca caatcatcac catctccatg atcctgtgcg tgcctctgtt
     1921 ctttatgggc tgctctaccc caacagtggc cgaggtgtac ccacccagca caagctcctc
     1981 tatccacccc cagtcccctg cctgtcggag agactgcagc tgtccagatt ccatttttca
     2041 ccccgtgtgc ggcgacaacg gcatcgagta cctgtcccca tgccacgcag gatgttctaa
     2101 catcaatatg agctccgcca cctctaagca gctgatctat ctgaattgca gctgcgtgac
     2161 cggaggaagc gcctccgcca agacaggatc ctgccctgtg ccatgtgccc acttcctgct
     2221 gccagccatc tttctgatca gcttcgtgtc cctgatcgcc tgtatctctc acaaccccct
     2281 gtacatgatg gtgctgagag tggtgaatca ggaggagaag agctttgcca tcggcgtgca
     2341 gttcctgctg atgaggctgc tggcatggct gccatcccct gccctgtatg gcctgacaat
     2401 cgaccactct tgcatcagat ggaacagcct gtgcctgggc aggcgcggag catgcgccta
     2461 ctatgacaat gatgccctga gggatcgcta cctgggcctg cagatgggct ataaggccct
     2521 gggcatgctg ctgctgtgct tcatcagctg gagggtgaag aagaacaagg agtataatgt
     2581 gcagaaggcc gccggcctga tctaagaccc agctttcttg tacaaagttg gcattataag
     2641 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2701 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2761 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2821 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     2881 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     2941 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     3001 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     3061 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     3121 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     3181 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     3241 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     3301 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     3361 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     3421 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     3481 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     3541 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     3601 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     3661 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     3721 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     3781 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3841 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3901 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3961 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4021 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4081 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4141 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4201 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4261 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4321 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4381 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4441 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
