LOCUS       sequence-435719-        4320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC17A8_STOP
FEATURES             Location/Qualifiers
     source          1..4320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2445..2544)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2561..2580)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2562..2580)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2585..2601)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2585..2601)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2703..2722)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2714..3523
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2781..2800)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3670..4258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4159..4178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgccat tcaaggcctt tgacacattc aaggagaaga tcctgaagcc
      721 cggcaaggag ggcgtgaaga acgccgtggg cgatagcctg ggcatcctgc agaggaagat
      781 cgacggcacc acagaggagg aggataacat cgagctgaat gaggagggca ggcccgtgca
      841 gacctccagg ccttctcccc ctctgtgcga ctgtcactgc tgtggcctgc ctaagaggta
      901 catcatcgcc atcatgtctg gcctgggctt ttgcatcagc ttcggcatcc gctgtaacct
      961 gggcgtggcc atcgtggaga tggtgaacaa ttccacagtg tacgtggacg gcaagcccga
     1021 gatccagaca gcccagttta attgggatcc tgagaccgtg ggcctgatcc acggcagctt
     1081 cttttggggc tatatcatga cccagatccc aggcggcttt atctccaaca agttcgccgc
     1141 caatagagtg tttggcgccg ccatcttcct gacatccacc ctgaatatgt tcatcccatc
     1201 tgccgcaagg gtgcactacg gatgcgtgat gtgcgtgcgg atcctgcagg gactggtgga
     1261 gggcgtgacc tatcctgcct gccacggcat gtggtctaag tgggcaccac cactggagcg
     1321 gagcagactg gccaccacat ccttttgtgg atcttacgca ggagcagtgg tggcaatgcc
     1381 actggccggc gtgctggtgc agtatatcgg ctggagctcc gtgttttaca tctatggcat
     1441 gttcggcatc atctggtaca tgttctggct gctgcaggca tatgagtgcc ctgcagcaca
     1501 cccaaccatc tctaacgagg agaagacata catcgagacc agcatcggcg agggcgccaa
     1561 tgtggtgtct ctgagcaagt tttccacacc atggaagagg ttctttacct ctctgcccgt
     1621 gtatgccatc atcgtggcca acttttgtcg cagctggaca ttctacctgc tgctgatctc
     1681 ccagcctgcc tatttcgagg aggtgttcgg ctttgccatc tccaaagtgg gcctgctgtc
     1741 tgccgtgcct cacatggtca tgaccatcgt ggtgccaatc ggaggacagc tggcagacta
     1801 cctgaggagc cgccagatcc tgaccacaac cgccgtgcgg aagatcatga attgcggcgg
     1861 ctttggcatg gaggccacac tgctgctggt ggtgggcttt agccacacca agggcgtggc
     1921 catctccttc ctggtgctgg ccgtgggctt tagcggcttc gccatctccg gcttcaacgt
     1981 gaatcacctg gatatcgccc ctagatatgc cagcatcctg atgggcatct ccaacggcgt
     2041 gggcaccctg tctggcatgg tgtgcccact gatcgtgggc gccatgacac ggcacaagac
     2101 cagagaggag tggcagaacg tgttcctgat cgccgccctg gtgcactact ccggcgtgat
     2161 cttttatggc gtgttcgcct ctggcgagaa gcaggagtgg gccgacccag agaacctgtc
     2221 cgaggagaag tgcggcatca tcgaccagga tgagctggcc gaggagatcg agctgaacca
     2281 cgagagcttt gcctccccca agaagaagat gtcttacggc gccacaagcc agaattgtga
     2341 ggtgcagaag aaggagtgga agggacagag gggagccaca ctggatgagg aggagctgac
     2401 cagctatcag aacgaggaga gaaatttctc taccatcagc taagacccag ctttcttgta
     2461 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2521 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2581 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2641 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2701 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     2761 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2821 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2881 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2941 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3001 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3061 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3121 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3181 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3241 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3301 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3361 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3421 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3481 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3541 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3601 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3661 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3721 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3781 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3841 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3901 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3961 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4021 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4081 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4141 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4201 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4261 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
