LOCUS       sequence-435730-       13739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 and Bxb1 integrase construct for tracking of stomata 
            development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, 
            no tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13739)
  TITLE     Nemhauser Lab Plasmids 2024
REFERENCE   2  (bases 1 to 13739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10
FEATURES             Location/Qualifiers
     source          1..13739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(957..976)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(958..976)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(983..1000)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(983..999)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(992..1014)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1217..1562
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1505..1525
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1582..2607
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    2839..3015
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    3023..3045
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      3172..3607
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(3887..3906)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3898..4713
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3898..4713
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3971..3990)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6222..6810
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6711..6730
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    6901..6925
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(7608..9110)
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISGAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     CDS             complement(11323..13140)
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 gtttccaagt atatatatat acaaaaaaac aaacaaaaaa aaaacatttg aaattagtga
       61 gttcacattt attttagcgc ctcaatcgtg agacaatcaa ttgaatgatg tgttgtacat
      121 tcacattata gatcattcat tattcaaaaa ataaaaagat acatatttag agacttttta
      181 tgattgatag accaatttca gacatgttat cgccagtcga tttaaaaata cagattttca
      241 cttaaagcat atactttggc ctttgtttat gtcatggtgt aatctaacca acatttaagc
      301 ccaaaaagaa gaacaaaatt cgattccaca attctgaaag aaaatccgtt ccttcttctt
      361 tcccgagcca tacatacaat ttaattaatt caatttagta atgttagttc agttcaactg
      421 gatccctaac atatttcata aacacctgat gagatggcat aagaattcaa tttcttataa
      481 aaccaaccca aaatagaata tgaatttagc aatattagta agcattatta ataagatggg
      541 atataatgtg tatatatata tatatatcac ctcttttgta agttaatctt tatattgata
      601 cagggagcaa acgaatttgt tttcaagaaa acaaaagaca agggatagat ctaaggcaat
      661 aaatagttga tgtcatacta gacgtgcctc attttgtctc atcaaaatca aattgcatct
      721 tgaggagcca cccttcattt gaactttccc tttcaaaagt ttttgaaata tttgtacata
      781 aatataaata ttgcttgaca ttttcttttt tgataatcca ttcaaatgtt ttagaaaata
      841 aacgaaaatt taagaagatg gagccaagac aaaccaggta gtcgttaggt ttgaagttaa
      901 tgagttggct gtcagatatt atttacctga ccatgtcttt ttagtaacgc tccaagccct
      961 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1021 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1081 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     1141 aattgtaagc gttaatgtta tcgtacccct actccaaaaa tgtcaaagat acagtctcag
     1201 aagaccaaag ggctattgag acttttcaac aaagggtaat ttcgggaaac ctcctcggat
     1261 tccattgccc agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct
     1321 acaaatgcca tcattgcgat aaaggaaagg ctatcattca agatgcctct gccgacagtg
     1381 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
     1441 cgtcttcaaa gcaagtggat tgatgtgaca tctccactga cgtaagggat gacgcacaat
     1501 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
     1561 cagcccaagc tgatcccccc tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     1621 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     1681 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     1741 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     1801 attccggaag tgcttgacat tggggagttc agcgagagcc tgacctattg catctcccgc
     1861 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttcttcag
     1921 ccggtcgcgg aggctatgga tgctatcgct gcggccgatc ttagccagac gagcgggttc
     1981 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     2041 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     2101 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     2161 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     2221 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     2281 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     2341 ccggagcttg caggatcgcc aaggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     2401 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     2461 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     2521 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     2581 agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgaacaagag ctgatttcga
     2641 gaacgcctca gccagcaact cgcgcgagcc tagcaaggca aatgcgagag aacggcctta
     2701 cgcttggtgg cacagttctc gtccacagtt cgctaagctc gctcggctgg gtcgcgggag
     2761 ggccggtcgc agtgattcag gaattaattc ggtacgctga aatcaccagt ctctctctac
     2821 aaatctatct ctctctattt tctccataaa taatgtgtga gtagtttccc gataagggaa
     2881 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     2941 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     3001 actaaaatcc agatcgataa cgttacacca caatatatcc tgccaagatc taattccggg
     3061 gatcggaaat ccagaagccc gagaggttgc cgcctttcgg gctttttctt tttcaaaaaa
     3121 aaaaatttat aaaacgatct gttgcggccg gccgccgggt tgtgggcaaa ggcgctcgac
     3181 ggtgggcaac cgcttgcggt tgtccacggg cggagccggt gcgcgtagcg cattgtccac
     3241 aagccaaggg cgaccaataa ttgatatata tattcataat tgaaaagcta attgaacata
     3301 ctacttgctg taactacttg ccggagcgag gggtgtttgc aagctgttga tctgaaaggg
     3361 ctattagcgt tctcacgtgc ctttttgatt agcgatttca cgtgacctta ttagcgattt
     3421 cacgtactcc gattagcgat ttcacgtacc ctgattagcg atttcacgtg gatagttttt
     3481 ggagcgggcc ggaaagcccc gtgaatcaag gctttgcggg gcattagcgg tttcacgtgg
     3541 ataactaccc tctatccaca ggcttccggg gataaaaaag cccgctcgac ggcgggctgt
     3601 tggatgggga tcgcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg
     3661 atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa
     3721 cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt
     3781 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     3841 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     3901 agccatattc aacgggaaac gtcttgctca aggccgcgat taaattccaa catggatgct
     3961 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctac
     4021 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     4081 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     4141 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     4201 ccagggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     4261 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     4321 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     4381 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     4441 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     4501 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     4561 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     4621 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     4681 cagtttcatt tgatgctcga tgagtttttc taatcactag accaatgtta cacatatata
     4741 ctttagactg atgaatcccc taatgatttt ggtaaaaatc attaagttaa ggtggataca
     4801 catcttgtca tatgatcaaa tggtttcgcg aaaaatcaat aatcagacaa caagatgtgc
     4861 gaactcgata ttttacacga ctctctttac caattctgcc ccgaattaca cttaaaacga
     4921 ctcaacagct taacgttggc ttgccacgca ttacttgact gtaaaactct cactcttacc
     4981 gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac ataacatcaa acgaatcgac
     5041 cgattgttag gtaatcgtca cctccacaaa gagcgactcg ctgtataccg ttggcatgct
     5101 agctttatct gttcgggcaa tacgatgccc attgtacttg ttgactggtc tgatattcgt
     5161 gagcaaaaac gacttatggt attgcgagct tcagtcgcac tacacggtcg ttctgttact
     5221 ctttatgaga aagcgttccc gctttcagag caatgttcaa agaaagctca tgaccaattt
     5281 ctagccgacc ttgcgagcat tctaccgagt aacaccacac cgctcattgt cagtgatgct
     5341 ggctttaaag tgccatggta taaatccgtt gagaagctgg gttggtactg gttaagtcga
     5401 gtaagaggaa aagtacaata tgcagaccta ggagcggaaa actggaaacc tatcagcaac
     5461 ttacatgata tgtcatctag tcactcaaag actttaggct ataagaggct gactaaaagc
     5521 aatccaatct catgccaaat tctattgtat aaatctcgct ctaaaggccg aaaaaatcag
     5581 cgctcgacac ggactcattg tcaccacccg tcacctaaaa tctactcagc gtcggcaaag
     5641 gagccatggg ttctagcaac taacttacct gttgaaattc gaacacccaa acaacttgtt
     5701 aatatctatt cgaagcgaat gcagattgaa gaaaccttcc gagacttgaa aagtcctgcc
     5761 tacggactag gcctacgcca tagccgaacg agcagctcag agcgttttga tatcatgctg
     5821 ctaatcgccc tgatgcttca actaacatgt tggcttgcgg gcgttcatgc tcagaaacaa
     5881 ggttgggaca agcacttcca ggctaacaca gtcagaaatc gaaacgtact ctcaacagtt
     5941 cgcttaggca tggaagtttt gcggcattct ggctacacaa taacaaggga agacttactc
     6001 gtggctgcaa ccctactagc tcaaaattta ttcacacatg gttacgcttt ggggaaatta
     6061 tgaggggatc tctcagtact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6121 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6181 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6241 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6301 gatcaagagc taccaactct tcttccgaag gtaactggct tcagcagagc gcagatacca
     6361 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6421 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6481 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6541 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6601 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6661 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6721 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6781 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6841 ctggcctttt gctggccttt tgctcacatg agatctcaaa caaacacata cagcgactta
     6901 gtttacccgc caatatatcc tgtcaaggcc ttcatgttct ttcctgcgtt atcccctgat
     6961 tctgtggtag cgaacataca ctttcacttg tgatcatcaa tttacttgag agtcgtttct
     7021 tcaaagtttc aaactttcca caacagaaac tctacttaag atgatcaagg cattgtcaaa
     7081 tgaaatgctt tggaaggaag aaactgcctc tccttcgtta aaccaagctc agtatcttgc
     7141 agtttcctct catcagacat atcaatgctt ggacaaaggt acgaaccatt ttctacattt
     7201 gtgggacatt acaggcattt cttagcaaca acgaatgttc atcatactca gaacctaaaa
     7261 gattaacatg ttcaagccat ccttagaacc caacagctca acactttcgc tacatctatt
     7321 actagaggcc aacaattgga ctatttacaa gtttcgcaga actgcactaa acagagtcga
     7381 gtcactttgc aggcgtatta aacaaaagaa gcacagataa taaaaacgaa gcgatgataa
     7441 agaagaagtt cgacttgtca ttagaaagaa agagataaca ggaacggaaa catagtagaa
     7501 cacttattca tcagggatta tacaaggccc caaaacacaa accaccaaag ttttacatga
     7561 aacgaaacat tgaacttctt aagcataaca gagacgagat aagcttatta cgacatcccg
     7621 gtgtgtagcc gttcgaccac gctgccgagc ctgagatgct gctcgtactc ctgaagatcc
     7681 ccgaagtcga tcgtgcgagt cagcccgccg cggacgtcga acgtcagccg aacgttcatc
     7741 gaccgaagcc aggtgttctt tgccgcggtg tcctgctccc gccaccagtc cccgaaccgc
     7801 tgcccggttt cgcgccactc ccagccagac gggcgagcct ccaggccctc cagctcctct
     7861 tgccgcgcgg ccagcgccgc aatacgggca tccagtgctt ctcgctgcgg agagcccgcc
     7921 cggtaggccg gggagccgat cagcgacgtc aggtccacca gctccgcgtt cacctccgcg
     7981 agttcgaccg cggagtccga gcccgctacc cagactttct ccagacgctc cgcgtccccg
     8041 agcagatcca gtacctgctc ctcgcagaac gcgtcccact cggccatcgc caccgtgccg
     8101 ttcccgcagt gcttcgggaa ccccatcgag cggcagcggt agcgcgggtg cttacgtcct
     8161 cccccggcga acttgtacgc gggctccccg cacaccgcgc agaacaacac ccgcagcagc
     8221 agcgacgggg tagacaccgc gggcttcgcc cgggaggtct tcacgagctc ggcgcgcagc
     8281 gcctccagct gctcacgggt caggatcggc tcagcccgca ccagcggggc tccgtcgtcg
     8341 tctcggacgg tcttaccgtt cagagtcgcg tacccgagca tcgccccgga gatcatcgat
     8401 cgcttcagcg cggtagccga ccactcccgg ccctgcggct cgcggccttg cagctgcgcg
     8461 aagtagtcct tcggcgacag gacaccacgc cggttcaggt cgtgggccac cagatgcagc
     8521 ggctcgtggt tgtcgacgac gcggtgatac acctcgagga tgcgctctcg ctgcacaggg
     8581 tccggcacca gccgccactc cccgtccacg cgcgtaggca ggtatcccca cggcggcagg
     8641 gatcctcggt atttcccggc gcggatattg aaatgcgcag ccgaacggtt ccgctctttg
     8701 atcgcttcta attccatctg cgccaccgtt cccataagcg cgatgacgac cgccgcaaac
     8761 ggcgtcgtcg tatcgaagtg cgcttcggtc gcggagacga ccagcttctt gtggtcctcg
     8821 gcccagtgga ccagctgctg aagatgccgg atcgatcggg tcaaccgatc tacccggtac
     8881 gccacgatca cgtcaaacgg ttgctcctcg aacgctagcc accgggccag gttcggtctg
     8941 cgcttccggt cgaacggatc gaccgccccg gagacgtcca gatcctccgc taccccgacg
     9001 acgtcccagc cgcgctgggc gcagagctgc tggcaagact ccagctgacg ctccggtgaa
     9061 gtcgtagcat cggtgacgcg ggacaggcgg atgactacca gggctctcat tgatacttaa
     9121 ttgatcaaga ttctcttctg gagttcctat attgatctat caacgaagaa gatgatctta
     9181 tgttttcact ttgttcgaca cattcttcaa aacaaagtcg tatttataca gccgcaacaa
     9241 caagtgataa ttagggttta gaatttcttt tcaaggttca taaatgcact atgtcgctgt
     9301 ctcgtgacat tcttttgtct cttcttgatt tttctctttc tctctcttat tactatcgat
     9361 atgtattaga acaacgtaaa gaaaaaggta tgcatacaca aagatacgtc acgtttgtga
     9421 agttggatac atttacattt acattcactg cgtgaggtct tttatgaatt tcaatatcgt
     9481 attttcattt agagtgcatg cctttgcacc accttctttt acttctagtt taatttctac
     9541 acttgtgcca cttctttgtt cttaattacc gcgtgtcctc acaagaaaaa atggatttct
     9601 tttactcttt attaacagat aatacactga tacatacaca aagtaaaaaa acattgaatc
     9661 ggacatatac tggtggaaag gtttagtttg tattgagatg ttttacagtt ttcatggatc
     9721 gatgaaggaa aataagtaca tcaaaagccg ttttttttgg cgtaacatat gttttttact
     9781 acttactatt agcaaacata ttgggtagta tctgattgga aatctacgaa atttcgaagt
     9841 gatgaaatga ttatgtgatt tcgcatgttt ctatatgaca tcgtttttgt gtaattatgt
     9901 ttcacatctt ctatcctatt gactttgtaa gataaatgac aacattgaca agtaacaaaa
     9961 tataaatagt tacgacatag tttgtagttg aattacaact ttttgttttc cttaatcact
    10021 atgtcattta ataccacaaa tttctcattc gtgatgattc catcattttc cttgatcact
    10081 agtcggaacc aatttccatt ggttaatgga taactttttg catgaaatat aggttctaca
    10141 agaaaacata aattgatata acctttggag caaagataaa gttcagttgt tcagctacct
    10201 ggacggctca ttaagattcc tcccgagaag tattcttaaa ttatattatt tatttttaat
    10261 aaagagatat tcgtaaaatt tcggctctgt tttgcaaagt taacaatatc tcaattcata
    10321 ttacaaactt cattaaataa ggtttaacct aaccaaaaaa attgctatag tgacaatatt
    10381 ttttctatga aacaaaccca cttggataac acttctattt gtggttttgt ttggacaaac
    10441 gtttattttt atggttcata tatgcatatc cacatataca ttatctccga agtcccaaaa
    10501 ctgtggaagc agctggaatg aaaatgttgg tgacaaatgt ttgtagccgc aaatgttagg
    10561 ctacaatacg tataagacat gcaaggtgca tgccccattt aattacgagt aaagaaatct
    10621 aactcctact tcgatattca atttgttcat ggtctatcac gttatataca atattgatcg
    10681 acaaggcaat gaaacaagaa aaaaaaaaaa aaaaactcac gttcgatcaa gatatcggtt
    10741 aacattcaat gcaagggtcg aaaaccaaac caaccaattt tcagtctggt ttgggtatgg
    10801 gattggtttg gaagccattt gtttgtcttt aacaacggct cacaacctgt gaccgtagag
    10861 agcctcaatg catggaacat gcagaagcct ctgaagatga accaaggtgc aaagtgatca
    10921 catcattctt taattttttc gttgacaaat ttaattcatt cgttaaaaca gagagtctgt
    10981 cacaatatat tgtagaaaaa aggaacctat tgtgtttctt ttacaatcaa tacaatttgt
    11041 acatagttta tcactactaa caatgtattg atcttatatt ttattaatga aacacgtctc
    11101 ctaatcacat aaacggtcat tatttcacga tacttgtata gattggaatt aagcaagaat
    11161 cttccgtaat cgattcagaa taatagaaaa ccaaatcctt ttactgacat tatcttttaa
    11221 catagaaagt cactgaaaca agaaaaacaa accctatcaa agggaagaca acaaaaaaaa
    11281 gcaagcaaga gatgggattc ttgataagag tctctaagct tattatgctg ccacatcctc
    11341 ggtaccatct tgtgcatcgt cttcatcatc atcggttgga ggctttgccc aggtaatact
    11401 ggctctcttt tcgatgggag tgccctgtcc tcgacccgta gtggacttag taactacaat
    11461 cttgtcaaca aatagaccca caaacactct cttatcgtct acgctagctc ttccccacca
    11521 gctctttgga cctgtaggat cagcgtcggc atcctcagga aaccattgat cgagtggaag
    11581 ctttggggcc tcggcggcct caagctctgc gagtcgctcc tctgcgcctt gctgtctcaa
    11641 agttagtgca gcttgttgct tcctgaaatg cttccgacca actggtccat catatgctcc
    11701 ggcagcccta tcttcgtaaa gttcttccaa tgcattcaag gcgtctgctc tttcggctac
    11761 caaattagct ctttcgccac ttttttctgg tgcctctgtt agcttaccaa aacgccgggc
    11821 agcctcccaa agaagagcta aagtttcctc atccccttca gcgtgtctaa tcttgttgaa
    11881 aatacgttcg gccacaaatt tatcaagagc agccatagaa acgttgcatg taccctcatg
    11941 ctgtcctgga gcagaagggt ccaccacttt acgacgtctg cacctgtaag aatccttgat
    12001 tgactcttct ccacgttttg atgtcataac agccccacac tcacaataaa gcttatccat
    12061 tgctgacaaa attgcctgtc cccgagataa accctttcca cgccctctac catcaagcca
    12121 ggcctgcaac tcgtaccact cggcaggttc gatgatcggt ccacagtcta attcaactgg
    12181 acgcaaagta ataggatccc tttggatcct ataaccttcg atttttgtag taggagtacc
    12241 atcaggtttt ttcttataaa tgacttctgc ggcgaatcca gcaatacgcg gatcccgaag
    12301 gattctcata actgtagcgg gatcccaagc ggaagatgca gtcttcttac cgattgtttc
    12361 ccctcgagtt ggcactgcgt cagcatccat ccttttacaa agcccagtga tagatccagg
    12421 gtggatagca gcctggctac ctggcttaaa tggaagatgt ttgtgagttt tgatctctct
    12481 ccaccaccat ctaataacgt caggttcgaa ctcaaaaggc cctgtgagag gggttgtgct
    12541 atgagctagc ttattaataa ccacgttcac catcctacca tttctggtaa tctcttttgt
    12601 ctcactcacg agttcgaaac catacggagc ttttcctcct acatagcctc caagctccct
    12661 ttgcaggttc ttggtgtcca agattttagc actcttcagt gatgactctt tatggctagc
    12721 gtcgagtcgc ataataaggt gtataaggtc catcacatta ccctgcctga atacaccttc
    12781 ttgtgtactt acgatagtaa caccgagtgc aagtagctcg ctcacgatcg gaattgcatc
    12841 cataactttg agtctactga acctagatac atcataaact attatcatat ttagtcgacc
    12901 ggcccgacac tcatttaata ttctttcaaa ttcaggccgt tcagcagtac caaaggctga
    12961 tgtaccagga gcctcagaga aatgacccac aaatctaaac ctacccccat ctctctctac
    13021 ctctctttgt aaatcagcag ctttatcctc gttggcagat ctctgtgtag ctggagaggc
    13081 ggcagaagag ttctctcttt ctcttgattg tctatcataa gcgccggcat acgtgtccat
    13141 tcgtgattag agatatatcc ttctctctct ctcttcctcg ttcgttgttt tgtcttatgg
    13201 ttggattttt tttttaaaca gcaaaataaa agaaagattt agggaaagaa agagacgcca
    13261 aaagacatat caagttgaga aagagaaata aagagagtga atgccaaaag gagtaaatat
    13321 gagggactcg cattcatcga ggactaaact atcgctatca aatttcaatt tttattatta
    13381 tattttgata gggttattta ttatgcggtt tgtgccgttg gatgaaattt gggggaggat
    13441 ctaggttttt cgtgtctcat ttatgtttta gatataaata tgcatctaca tatatatata
    13501 tggtacgtct tattgtatac tttatattac agagataaag gttgcatatt gcaaagggaa
    13561 agatacataa attccttagg aaaagatttt tatgggggta aaattttgaa taatcacaag
    13621 ctcggtataa ttgttttttg tacacacaaa catgtaatac acataaacac ggacatgtac
    13681 gttctttgta tttaatttac gtatatgttc gggttgcgaa tatggtccga ccaatcccg
//
