LOCUS       sequence-435731-       13046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral 
            root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for
            Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13046)
  TITLE     Nemhauser Lab Plasmids 2024
REFERENCE   2  (bases 1 to 13046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L1PB26- pAHP6--PhiC31-NLS-tUBQ1 +
            pGATA23--Bxb...
FEATURES             Location/Qualifiers
     source          1..13046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    44..68
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(159..747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(239..258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2256..3071)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2256..3071)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2979..2998
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3063..3082
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3362..3797
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3924..3946
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    3954..4130
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4362..5387)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5407..5752)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5444..5464)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     5955..5977
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5969..5986
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5970..5986
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5993..6012
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5993..6011
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7615..9429
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             9430..9450
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10768..12270
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISGAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
ORIGIN
        1 accacagaat caggggataa cgcaggaaag aacatgaagg ccttgacagg atatattggc
       61 gggtaaacta agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa aggccagcaa
      121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaagaag agttggtagc
      661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
      781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
      841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tactgagaga
      901 tcccctcata atttccccaa agcgtaacca tgtgtgaata aattttgagc tagtagggtt
      961 gcagccacga gtaagtcttc ccttgttatt gtgtagccag aatgccgcaa aacttccatg
     1021 cctaagcgaa ctgttgagag tacgtttcga tttctgactg tgttagcctg gaagtgcttg
     1081 tcccaacctt gtttctgagc atgaacgccc gcaagccaac atgttagttg aagcatcagg
     1141 gcgattagca gcatgatatc aaaacgctct gagctgctcg ttcggctatg gcgtaggcct
     1201 agtccgtagg caggactttt caagtctcgg aaggtttctt caatctgcat tcgcttcgaa
     1261 tagatattaa caagttgttt gggtgttcga atttcaacag gtaagttagt tgctagaacc
     1321 catggctcct ttgccgacgc tgagtagatt ttaggtgacg ggtggtgaca atgagtccgt
     1381 gtcgagcgct gattttttcg gcctttagag cgagatttat acaatagaat ttggcatgag
     1441 attggattgc ttttagtcag cctcttatag cctaaagtct ttgagtgact agatgacata
     1501 tcatgtaagt tgctgatagg tttccagttt tccgctccta ggtctgcata ttgtactttt
     1561 cctcttactc gacttaacca gtaccaaccc agcttctcaa cggatttata ccatggcact
     1621 ttaaagccag catcactgac aatgagcggt gtggtgttac tcggtagaat gctcgcaagg
     1681 tcggctagaa attggtcatg agctttcttt gaacattgct ctgaaagcgg gaacgctttc
     1741 tcataaagag taacagaacg accgtgtagt gcgactgaag ctcgcaatac cataagtcgt
     1801 ttttgctcac gaatatcaga ccagtcaaca agtacaatgg gcatcgtatt gcccgaacag
     1861 ataaagctag catgccaacg gtatacagcg agtcgctctt tgtggaggtg acgattacct
     1921 aacaatcggt cgattcgttt gatgttatgt tttgttctcg ctttggttgg caggttacgg
     1981 ccaagttcgg taagagtgag agttttacag tcaagtaatg cgtggcaagc caacgttaag
     2041 ctgttgagtc gttttaagtg taattcgggg cagaattggt aaagagagtc gtgtaaaata
     2101 tcgagttcgc acatcttgtt gtctgattat tgatttttcg cgaaaccatt tgatcatatg
     2161 acaagatgtg tatccacctt aacttaatga tttttaccaa aatcattagg ggattcatca
     2221 gtctaaagta tatatgtgta acattggtct agtgattaga aaaactcatc gagcatcaaa
     2281 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     2341 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     2401 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     2461 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     2521 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     2581 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     2641 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     2701 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     2761 ttccctggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     2821 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     2881 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     2941 tacaatcggt agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     3001 tataaatcag catccatgtt ggaatttaat cgcggccttg agcaagacgt ttcccgttga
     3061 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     3121 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     3181 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac
     3241 gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa
     3301 gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat tcaggcgatc
     3361 cccatccaac agcccgccgt cgagcgggct tttttatccc cggaagcctg tggatagagg
     3421 gtagttatcc acgtgaaacc gctaatgccc cgcaaagcct tgattcacgg ggctttccgg
     3481 cccgctccaa aaactatcca cgtgaaatcg ctaatcaggg tacgtgaaat cgctaatcgg
     3541 agtacgtgaa atcgctaata aggtcacgtg aaatcgctaa tcaaaaaggc acgtgagaac
     3601 gctaatagcc ctttcagatc aacagcttgc aaacacccct cgctccggca agtagttaca
     3661 gcaagtagta tgttcaatta gcttttcaat tatgaatata tatatcaatt attggtcgcc
     3721 cttggcttgt ggacaatgcg ctacgcgcac cggctccgcc cgtggacaac cgcaagcggt
     3781 tgcccaccgt cgagcgcctt tgcccacaac ccggcggccg gccgcaacag atcgttttat
     3841 aaattttttt ttttgaaaaa gaaaaagccc gaaaggcggc aacctctcgg gcttctggat
     3901 ttccgatccc cggaattaga tcttggcagg atatattgtg gtgtaacgtt atcgatctgg
     3961 attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca
     4021 aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa ccctataaga
     4081 accctaattt cccttatcgg gaaactactc acacattatt tatggagaaa atagagagag
     4141 atagatttgt agagagagac tggtgatttc agcgtaccga attaattcct gaatcactgc
     4201 gaccggccct cccgcgaccc agccgagcga gcttagcgaa ctgtggacga gaactgtgcc
     4261 accaagcgta aggccgttct ctcgcatttg ccttgctagg ctcgcgcgag ttgctggctg
     4321 aggcgttctc gaaatcagct cttgttcggt cggcatctac tctattcctt tgccctcgga
     4381 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
     4441 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
     4501 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
     4561 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccttgg cgatcctgca
     4621 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
     4681 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
     4741 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
     4801 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
     4861 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
     4921 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
     4981 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatagcatc catagcctcc
     5041 gcgaccggct gaagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
     5101 tgtgcacggc gggagatgca ataggtcagg ctctcgctga actccccaat gtcaagcact
     5161 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
     5221 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
     5281 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
     5341 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatagg ggggatcagc
     5401 ttgggctgtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
     5461 atagtgggat tgtgcgtcat cccttacgtc agtggagatg tcacatcaat ccacttgctt
     5521 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     5581 tttgggacca ctgtcggcag aggcatcttg aatgatagcc tttcctttat cgcaatgatg
     5641 gcatttgtag gagccacctt ccttttctac tgtcctttcg atgaagtgac agatagctgg
     5701 gcaatggaat ccgaggaggt ttcccgaaat taccctttgt tgaaaagtct caatagccct
     5761 ttggtcttct gagactgtat ctttgacatt tttggagtag gggtacgata acattaacgc
     5821 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5881 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     5941 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     6001 ctcactatag ggcttggagc acggggcgca aagaagcatg acatacgaat gagacaattt
     6061 cagttttcgt atataataat tccataatca taatttaatc actagtttta attggtaaca
     6121 taaccttaaa aaagcatcca ctcattatgc gctccacttt actacttact ctgataacat
     6181 gaaaaagtat atgtttaata ctagctagtt gtttgttccg tttccaagca cgctctttct
     6241 tcttttcagt catttttaat ccgcatttac aataatattt agctattttc atatatcatt
     6301 ctatactagc tcacaataat tacatatgag cacgcttttt tgtgttcaaa tattataaat
     6361 cttcgttagt taccgctgct ttttataatt atgtatactt tctggttcca ggagactaat
     6421 taaggaagat tattagaggc ttcttatacg tgccatatga ttatttattt atgtactgtc
     6481 atggttcata tatagcgatg tgataccaat ataccaatca ccaaagttat gagaaaatca
     6541 cgtaaccaga ggtgttggta catacatttt ctgttaaaaa tagttaagag gacttgtaat
     6601 cttttgtgtt taactattga taatttttta ctttattcgg tgaaacaatt tacgcatgtt
     6661 tctgtgttgt gcttgtgttt cttgtattct tcttgtgtat agctttcctt ataagaaaga
     6721 aaaagaagaa gattaattga ttagatcgag aaagagaaag gccttttgag atagaaatgg
     6781 agaaaggatg gattgaattt gaagcataga gaatagcgaa cactcactgc aaagttagtg
     6841 ggaaagtcgc gcgtcatacg agggagcaac aaagtgtggg cttttctttt acagtctcag
     6901 aaactaatgc atgctcacat ttaaaaaaaa aaaaaaaaaa aacaattctt gtgttaaatc
     6961 aagagtggat taattatgag ataatctaat gtacattact gttatatgat tataacttga
     7021 cagaccaaat aatcatctta atcagacctg ataaaagaaa agagattaat ttcaacagtg
     7081 agaaattaaa gatagaaagt acatattatt ggtgtaaaag ttgttgattt aatttaaggc
     7141 agatgtatta agttaaatat gtaaatgtag aggctgcggg aagggtgacc agctggtctg
     7201 acagggtacg ccggttgtcg ggaggaagaa cgccgctatt ggcagagtca cgcggggtaa
     7261 ccggctggcg gctgtagccg gtcacagagt gtattaatat ggtcgtacaa tacttgtaga
     7321 gtgctttttc ctgtgacggt cccgtttatt ccccacaccc tcctcgattc tttcacttaa
     7381 aagtggggga aaaaggtaac ctgactctta gaaaatttga atacaattgt gggtcacctc
     7441 atctcatgta gtcatgtcta tacaattata aatacctctc aacaaaccca aaatcgaatt
     7501 ataagccttc tctagctaga atttgaagca agacctgttc acattagtaa aaacaaatat
     7561 atagaaagag gaaagattga agcggaaaac gggacaagac gggtgtgccg ttgaatggac
     7621 acgtatgccg gcgcttatga tagacaatca agagaaagag agaactcttc tgccgcctct
     7681 ccagctacac agagatctgc caacgaggat aaagctgctg atttacaaag agaggtagag
     7741 agagatgggg gtaggtttag atttgtgggt catttctctg aggctcctgg tacatcagcc
     7801 tttggtactg ctgaacggcc tgaatttgaa agaatattaa atgagtgtcg ggccggtcga
     7861 ctaaatatga taatagttta tgatgtatct aggttcagta gactcaaagt tatggatgca
     7921 attccgatcg tgagcgagct acttgcactc ggtgttacta tcgtaagtac acaagaaggt
     7981 gtattcaggc agggtaatgt gatggacctt atacacctta ttatgcgact cgacgctagc
     8041 cataaagagt catcactgaa gagtgctaaa atcttggaca ccaagaacct gcaaagggag
     8101 cttggaggct atgtaggagg aaaagctccg tatggtttcg aactcgtgag tgagacaaaa
     8161 gagattacca gaaatggtag gatggtgaac gtggttatta ataagctagc tcatagcaca
     8221 acccctctca cagggccttt tgagttcgaa cctgacgtta ttagatggtg gtggagagag
     8281 atcaaaactc acaaacatct tccatttaag ccaggtagcc aggctgctat ccaccctgga
     8341 tctatcactg ggctttgtaa aaggatggat gctgacgcag tgccaactcg aggggaaaca
     8401 atcggtaaga agactgcatc ttccgcttgg gatcccgcta cagttatgag aatccttcgg
     8461 gatccgcgta ttgctggatt cgccgcagaa gtcatttata agaaaaaacc tgatggtact
     8521 cctactacaa aaatcgaagg ttataggatc caaagggatc ctattacttt gcgtccagtt
     8581 gaattagact gtggaccgat catcgaacct gccgagtggt acgagttgca ggcctggctt
     8641 gatggtagag ggcgtggaaa gggtttatct cggggacagg caattttgtc agcaatggat
     8701 aagctttatt gtgagtgtgg ggctgttatg acatcaaaac gtggagaaga gtcaatcaag
     8761 gattcttaca ggtgcagacg tcgtaaagtg gtggaccctt ctgctccagg acagcatgag
     8821 ggtacatgca acgtttctat ggctgctctt gataaatttg tggccgaacg tattttcaac
     8881 aagattagac acgctgaagg ggatgaggaa actttagctc ttctttggga ggctgcccgg
     8941 cgttttggta agctaacaga ggcaccagaa aaaagtggcg aaagagctaa tttggtagcc
     9001 gaaagagcag acgccttgaa tgcattggaa gaactttacg aagatagggc tgccggagca
     9061 tatgatggac cagttggtcg gaagcatttc aggaagcaac aagctgcact aactttgaga
     9121 cagcaaggcg cagaggagcg actcgcagag cttgaggccg ccgaggcccc aaagcttcca
     9181 ctcgatcaat ggtttcctga ggatgccgac gctgatccta caggtccaaa gagctggtgg
     9241 ggaagagcta gcgtagacga taagagagtg tttgtgggtc tatttgttga caagattgta
     9301 gttactaagt ccactacggg tcgaggacag ggcactccca tcgaaaagag agccagtatt
     9361 acctgggcaa agcctccaac cgatgatgat gaagacgatg cacaagatgg taccgaggat
     9421 gtggcagcac caaaaaaaaa gaggaaggtg taagcttaga gactcttatc aagaatccca
     9481 tctcttgctt gctttttttt gttgtcttcc ctttgatagg gtttgttttt cttgtttcag
     9541 tgactttcta tgttaaaaga taatgtcagt aaaaggattt ggttttctat tattctgaat
     9601 cgattacgga agattcttgc ttaattccaa tctatacaag tatcgtgaaa taatgaccgt
     9661 ttatgtgatt aggagacgtg tttcattaat aaaatataag atcaatacat tgttagtagt
     9721 gataaactat gtacaaattg tattgattgt aaaagaaaca caataggttc cttttttcta
     9781 caatatattg tgacagactc tctgttttaa cgaatgaatt aaatttgtca acgaaaaaat
     9841 taaagaatga tgtgatcact ttgcaccttg gttcatcttc agaggcttct gcatgttcca
     9901 tgcattgagg ctctctacgg tcacaggttg tgagccgttg ttaaagacaa acaaatggct
     9961 tcatgaataa tctttgcgta cacctaaatt ttatagtgaa aaatacataa ttttaaaact
    10021 ttacttttgt agagatgcct aaaaaaaaat ggtttaccac atggacattg ttatttgttc
    10081 agatctccaa cgtatagcaa tatttgtgat ttatagatat gtaatttggt gttcaaagaa
    10141 tgtgtagaac aattattctt atgggtatgc caagaaaagt tatataactt tattctatac
    10201 tgctttcacg ttaaataatg taacattatt aatgttgtcc ttttactttt ccttgaattg
    10261 atttgggaag aagactattg gcttccttat cacatcaaaa agtctatgag taagtgaaac
    10321 attggctggc ttttgcttca ttcctttgtc tctgtcttca tgtgaacaag tttgcagaaa
    10381 tttgtggttt ctttttttac tttcttttgt ctaatacaca cactctcacc actattataa
    10441 agaaaatcca tagatcccgg tcgtgcgtac tgccactttg ctttccaccc gacaatgatt
    10501 tgggagatgt tttgttttgt gtcatacatc taccaaaaaa gtcttaatca ttgttcatca
    10561 aaagagcgcc caagggctct ttcgtcttct taccacacgc acactagttc gagagggttt
    10621 ttaaggagct cttaagcctt tcttgatgac tcataccacg aacaagaacc aatcttgtca
    10681 cccatataat ctccgtattc agagaattga agagttcttg atcactactg ttgtatcaaa
    10741 aaggaactct aagaactaag attgaaaatg agagccctgg tagtcatccg cctgtcccgc
    10801 gtcaccgatg ctacgacttc accggagcgt cagctggagt cttgccagca gctctgcgcc
    10861 cagcgcggct gggacgtcgt cggggtagcg gaggatctgg acgtctccgg ggcggtcgat
    10921 ccgttcgacc ggaagcgcag accgaacctg gcccggtggc tagcgttcga ggagcaaccg
    10981 tttgacgtga tcgtggcgta ccgggtagat cggttgaccc gatcgatccg gcatcttcag
    11041 cagctggtcc actgggccga ggaccacaag aagctggtcg tctccgcgac cgaagcgcac
    11101 ttcgatacga cgacgccgtt tgcggcggtc gtcatcgcgc ttatgggaac ggtggcgcag
    11161 atggaattag aagcgatcaa agagcggaac cgttcggctg cgcatttcaa tatccgcgcc
    11221 gggaaatacc gaggatccct gccgccgtgg ggatacctgc ctacgcgcgt ggacggggag
    11281 tggcggctgg tgccggaccc tgtgcagcga gagcgcatcc tcgaggtgta tcaccgcgtc
    11341 gtcgacaacc acgagccgct gcatctggtg gcccacgacc tgaaccggcg tggtgtcctg
    11401 tcgccgaagg actacttcgc gcagctgcaa ggccgcgagc cgcagggccg ggagtggtcg
    11461 gctaccgcgc tgaagcgatc gatgatctcc ggggcgatgc tcgggtacgc gactctgaac
    11521 ggtaagaccg tccgagacga cgacggagcc ccgctggtgc gggctgagcc gatcctgacc
    11581 cgtgagcagc tggaggcgct gcgcgccgag ctcgtgaaga cctcccgggc gaagcccgcg
    11641 gtgtctaccc cgtcgctgct gctgcgggtg ttgttctgcg cggtgtgcgg ggagcccgcg
    11701 tacaagttcg ccgggggagg acgtaagcac ccgcgctacc gctgccgctc gatggggttc
    11761 ccgaagcact gcgggaacgg cacggtggcg atggccgagt gggacgcgtt ctgcgaggag
    11821 caggtactgg atctgctcgg ggacgcggag cgtctggaga aagtctgggt agcgggctcg
    11881 gactccgcgg tcgaactcgc ggaggtgaac gcggagctgg tggacctgac gtcgctgatc
    11941 ggctccccgg cctaccgggc gggctctccg cagcgagaag cactggatgc ccgtattgcg
    12001 gcgctggccg cgcggcaaga ggagctggag ggcctggagg ctcgcccgtc tggctgggag
    12061 tggcgcgaaa ccgggcagcg gttcggggac tggtggcggg agcaggacac cgcggcaaag
    12121 aacacctggc ttcggtcgat gaacgttcgg ctgacgttcg acgtccgcgg cgggctgact
    12181 cgcacgatcg acttcgggga tcttcaggag tacgagcagc atctcaggct cggcagcgtg
    12241 gtcgaacggc tacacaccgg gatgtcgtaa taagcttagt actatactac aacatttcca
    12301 tatttttttt agattgttag ctaatttccc ctggagataa ttgtaaattg tttcaatgag
    12361 aggaatatac aatacataga tcgtaattga tcaatgcgta tttgcatgtt ggtaatctcg
    12421 tctctgttat gcttaagaag ttcaatgttt cgtttcatgt aaaactttgg tggtttgtgt
    12481 tttggggcct tgtataatcc ctgatgaata agtgttctac tatgtttccg ttcctgttat
    12541 ctctttcttt ctaatgacaa gtcgaacttc ttctttatca tcgcttcgtt tttattatct
    12601 gtgcttcttt tgtttaatac gcctgcaaag tgactcgact ctgtttagtg cagttctgcg
    12661 aaacttgtaa atagtccaat tgttggcctc tagtaataga tgtagcgaaa gtgttgagct
    12721 gttgggttct aaggatggct tgaacatgtt aatcttttag gttctgagta tgatgaacat
    12781 tcgttgttgc taagaaatgc ctgtaatgtc ccacaaatgt agaaaatggt tcgtaccttt
    12841 gtccaagcat tgatatgtct gatgagagga aactgcaaga tactgagctt ggtttaacga
    12901 aggagaggca gtttcttcct tccaaagcat ttcatttgac aatgccttga tcatcttaag
    12961 tagagtttct gttgtggaaa gtttgaaact ttgaagaaac gactctcaag taaattgatg
    13021 atcacaagtg aaagtgtatg ttcgct
//
