LOCUS       sequence-435733-        9898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 pSPCH-PhiC31-tUBQ1, for early stage of stomata development 
            expression of PhiC31 integrase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9898)
  TITLE     Nemhauser Lab Plasmids 2024
REFERENCE   2  (bases 1 to 9898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L1P139 - pSPCH-PhiC31-tUBQ1
FEATURES             Location/Qualifiers
     source          1..9898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    44..68
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(159..747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(239..258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2256..3071)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2256..3071)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2979..2998
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3063..3082
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3362..3797
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3924..3946
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    3954..4130
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4362..5387)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5407..5752)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5444..5464)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     5955..5977
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5969..5986
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5970..5986
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5993..6012
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5993..6011
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7570..9387
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 accacagaat caggggataa cgcaggaaag aacatgaagg ccttgacagg atatattggc
       61 gggtaaacta agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa aggccagcaa
      121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaagaag agttggtagc
      661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
      781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
      841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tactgagaga
      901 tcccctcata atttccccaa agcgtaacca tgtgtgaata aattttgagc tagtagggtt
      961 gcagccacga gtaagtcttc ccttgttatt gtgtagccag aatgccgcaa aacttccatg
     1021 cctaagcgaa ctgttgagag tacgtttcga tttctgactg tgttagcctg gaagtgcttg
     1081 tcccaacctt gtttctgagc atgaacgccc gcaagccaac atgttagttg aagcatcagg
     1141 gcgattagca gcatgatatc aaaacgctct gagctgctcg ttcggctatg gcgtaggcct
     1201 agtccgtagg caggactttt caagtctcgg aaggtttctt caatctgcat tcgcttcgaa
     1261 tagatattaa caagttgttt gggtgttcga atttcaacag gtaagttagt tgctagaacc
     1321 catggctcct ttgccgacgc tgagtagatt ttaggtgacg ggtggtgaca atgagtccgt
     1381 gtcgagcgct gattttttcg gcctttagag cgagatttat acaatagaat ttggcatgag
     1441 attggattgc ttttagtcag cctcttatag cctaaagtct ttgagtgact agatgacata
     1501 tcatgtaagt tgctgatagg tttccagttt tccgctccta ggtctgcata ttgtactttt
     1561 cctcttactc gacttaacca gtaccaaccc agcttctcaa cggatttata ccatggcact
     1621 ttaaagccag catcactgac aatgagcggt gtggtgttac tcggtagaat gctcgcaagg
     1681 tcggctagaa attggtcatg agctttcttt gaacattgct ctgaaagcgg gaacgctttc
     1741 tcataaagag taacagaacg accgtgtagt gcgactgaag ctcgcaatac cataagtcgt
     1801 ttttgctcac gaatatcaga ccagtcaaca agtacaatgg gcatcgtatt gcccgaacag
     1861 ataaagctag catgccaacg gtatacagcg agtcgctctt tgtggaggtg acgattacct
     1921 aacaatcggt cgattcgttt gatgttatgt tttgttctcg ctttggttgg caggttacgg
     1981 ccaagttcgg taagagtgag agttttacag tcaagtaatg cgtggcaagc caacgttaag
     2041 ctgttgagtc gttttaagtg taattcgggg cagaattggt aaagagagtc gtgtaaaata
     2101 tcgagttcgc acatcttgtt gtctgattat tgatttttcg cgaaaccatt tgatcatatg
     2161 acaagatgtg tatccacctt aacttaatga tttttaccaa aatcattagg ggattcatca
     2221 gtctaaagta tatatgtgta acattggtct agtgattaga aaaactcatc gagcatcaaa
     2281 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     2341 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     2401 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     2461 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     2521 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     2581 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     2641 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     2701 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     2761 ttccctggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     2821 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     2881 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     2941 tacaatcggt agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     3001 tataaatcag catccatgtt ggaatttaat cgcggccttg agcaagacgt ttcccgttga
     3061 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     3121 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     3181 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac
     3241 gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa
     3301 gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat tcaggcgatc
     3361 cccatccaac agcccgccgt cgagcgggct tttttatccc cggaagcctg tggatagagg
     3421 gtagttatcc acgtgaaacc gctaatgccc cgcaaagcct tgattcacgg ggctttccgg
     3481 cccgctccaa aaactatcca cgtgaaatcg ctaatcaggg tacgtgaaat cgctaatcgg
     3541 agtacgtgaa atcgctaata aggtcacgtg aaatcgctaa tcaaaaaggc acgtgagaac
     3601 gctaatagcc ctttcagatc aacagcttgc aaacacccct cgctccggca agtagttaca
     3661 gcaagtagta tgttcaatta gcttttcaat tatgaatata tatatcaatt attggtcgcc
     3721 cttggcttgt ggacaatgcg ctacgcgcac cggctccgcc cgtggacaac cgcaagcggt
     3781 tgcccaccgt cgagcgcctt tgcccacaac ccggcggccg gccgcaacag atcgttttat
     3841 aaattttttt ttttgaaaaa gaaaaagccc gaaaggcggc aacctctcgg gcttctggat
     3901 ttccgatccc cggaattaga tcttggcagg atatattgtg gtgtaacgtt atcgatctgg
     3961 attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca
     4021 aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa ccctataaga
     4081 accctaattt cccttatcgg gaaactactc acacattatt tatggagaaa atagagagag
     4141 atagatttgt agagagagac tggtgatttc agcgtaccga attaattcct gaatcactgc
     4201 gaccggccct cccgcgaccc agccgagcga gcttagcgaa ctgtggacga gaactgtgcc
     4261 accaagcgta aggccgttct ctcgcatttg ccttgctagg ctcgcgcgag ttgctggctg
     4321 aggcgttctc gaaatcagct cttgttcggt cggcatctac tctattcctt tgccctcgga
     4381 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
     4441 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
     4501 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
     4561 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccttgg cgatcctgca
     4621 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
     4681 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
     4741 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
     4801 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
     4861 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
     4921 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
     4981 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatagcatc catagcctcc
     5041 gcgaccggct gaagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
     5101 tgtgcacggc gggagatgca ataggtcagg ctctcgctga actccccaat gtcaagcact
     5161 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
     5221 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
     5281 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
     5341 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatagg ggggatcagc
     5401 ttgggctgtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
     5461 atagtgggat tgtgcgtcat cccttacgtc agtggagatg tcacatcaat ccacttgctt
     5521 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     5581 tttgggacca ctgtcggcag aggcatcttg aatgatagcc tttcctttat cgcaatgatg
     5641 gcatttgtag gagccacctt ccttttctac tgtcctttcg atgaagtgac agatagctgg
     5701 gcaatggaat ccgaggaggt ttcccgaaat taccctttgt tgaaaagtct caatagccct
     5761 ttggtcttct gagactgtat ctttgacatt tttggagtag gggtacgata acattaacgc
     5821 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5881 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     5941 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     6001 ctcactatag ggcttggagc gttactaaaa agacatggtc aggtaaataa tatctgacag
     6061 ccaactcatt aacttcaaac ctaacgacta cctggtttgt cttggctcca tcttcttaaa
     6121 ttttcgttta ttttctaaaa catttgaatg gattatcaaa aaagaaaatg tcaagcaata
     6181 tttatattta tgtacaaata tttcaaaaac ttttgaaagg gaaagttcaa atgaagggtg
     6241 gctcctcaag atgcaatttg attttgatga gacaaaatga ggcacgtcta gtatgacatc
     6301 aactatttat tgccttagat ctatcccttg tcttttgttt tcttgaaaac aaattcgttt
     6361 gctccctgta tcaatataaa gattaactta caaaagaggt gatatatata tatatatata
     6421 cacattatat cccatcttat taataatgct tactaatatt gctaaattca tattctattt
     6481 tgggttggtt ttataagaaa ttgaattctt atgccatctc atcaggtgtt tatgaaatat
     6541 gttagggatc cagttgaact gaactaacat tactaaattg aattaattaa attgtatgta
     6601 tggctcggga aagaagaagg aacggatttt ctttcagaat tgtggaatcg aattttgttc
     6661 ttctttttgg gcttaaatgt tggttagatt acaccatgac ataaacaaag gccaaagtat
     6721 atgctttaag tgaaaatctg tatttttaaa tcgactggcg ataacatgtc tgaaattggt
     6781 ctatcaatca taaaaagtct ctaaatatgt atctttttat tttttgaata atgaatgatc
     6841 tataatgtga atgtacaaca catcattcaa ttgattgtct cacgattgag gcgctaaaat
     6901 aaatgtgaac tcactaattt caaatgtttt ttttttgttt gtttttttgt atatatatat
     6961 acttggaaac cgggattggt cggaccatat tcgcaacccg aacatatacg taaattaaat
     7021 acaaagaacg tacatgtccg tgtttatgtg tattacatgt ttgtgtgtac aaaaaacaat
     7081 tataccgagc ttgtgattat tcaaaatttt acccccataa aaatcttttc ctaaggaatt
     7141 tatgtatctt tccctttgca atatgcaacc tttatctctg taatataaag tatacaataa
     7201 gacgtaccat atatatatat gtagatgcat atttatatct aaaacataaa tgagacacga
     7261 aaaacctaga tcctccccca aatttcatcc aacggcacaa accgcataat aaataaccct
     7321 atcaaaatat aataataaaa attgaaattt gatagcgata gtttagtcct cgatgaatgc
     7381 gagtccctca tatttactcc ttttggcatt cactctcttt atttctcttt ctcaacttga
     7441 tatgtctttt ggcgtctctt tctttcccta aatctttctt ttattttgct gtttaaaaaa
     7501 aaaatccaac cataagacaa aacaacgaac gaggaagaga gagagagaag gatatatctc
     7561 taatcacgaa tggacacgta tgccggcgct tatgatagac aatcaagaga aagagagaac
     7621 tcttctgccg cctctccagc tacacagaga tctgccaacg aggataaagc tgctgattta
     7681 caaagagagg tagagagaga tgggggtagg tttagatttg tgggtcattt ctctgaggct
     7741 cctggtacat cagcctttgg tactgctgaa cggcctgaat ttgaaagaat attaaatgag
     7801 tgtcgggccg gtcgactaaa tatgataata gtttatgatg tatctaggtt cagtagactc
     7861 aaagttatgg atgcaattcc gatcgtgagc gagctacttg cactcggtgt tactatcgta
     7921 agtacacaag aaggtgtatt caggcagggt aatgtgatgg accttataca ccttattatg
     7981 cgactcgacg ctagccataa agagtcatca ctgaagagtg ctaaaatctt ggacaccaag
     8041 aacctgcaaa gggagcttgg aggctatgta ggaggaaaag ctccgtatgg tttcgaactc
     8101 gtgagtgaga caaaagagat taccagaaat ggtaggatgg tgaacgtggt tattaataag
     8161 ctagctcata gcacaacccc tctcacaggg ccttttgagt tcgaacctga cgttattaga
     8221 tggtggtgga gagagatcaa aactcacaaa catcttccat ttaagccagg tagccaggct
     8281 gctatccacc ctggatctat cactgggctt tgtaaaagga tggatgctga cgcagtgcca
     8341 actcgagggg aaacaatcgg taagaagact gcatcttccg cttgggatcc cgctacagtt
     8401 atgagaatcc ttcgggatcc gcgtattgct ggattcgccg cagaagtcat ttataagaaa
     8461 aaacctgatg gtactcctac tacaaaaatc gaaggttata ggatccaaag ggatcctatt
     8521 actttgcgtc cagttgaatt agactgtgga ccgatcatcg aacctgccga gtggtacgag
     8581 ttgcaggcct ggcttgatgg tagagggcgt ggaaagggtt tatctcgggg acaggcaatt
     8641 ttgtcagcaa tggataagct ttattgtgag tgtggggctg ttatgacatc aaaacgtgga
     8701 gaagagtcaa tcaaggattc ttacaggtgc agacgtcgta aagtggtgga cccttctgct
     8761 ccaggacagc atgagggtac atgcaacgtt tctatggctg ctcttgataa atttgtggcc
     8821 gaacgtattt tcaacaagat tagacacgct gaaggggatg aggaaacttt agctcttctt
     8881 tgggaggctg cccggcgttt tggtaagcta acagaggcac cagaaaaaag tggcgaaaga
     8941 gctaatttgg tagccgaaag agcagacgcc ttgaatgcat tggaagaact ttacgaagat
     9001 agggctgccg gagcatatga tggaccagtt ggtcggaagc atttcaggaa gcaacaagct
     9061 gcactaactt tgagacagca aggcgcagag gagcgactcg cagagcttga ggccgccgag
     9121 gccccaaagc ttccactcga tcaatggttt cctgaggatg ccgacgctga tcctacaggt
     9181 ccaaagagct ggtggggaag agctagcgta gacgataaga gagtgtttgt gggtctattt
     9241 gttgacaaga ttgtagttac taagtccact acgggtcgag gacagggcac tcccatcgaa
     9301 aagagagcca gtattacctg ggcaaagcct ccaaccgatg atgatgaaga cgatgcacaa
     9361 gatggtaccg aggatgtggc agcataataa gcttagagac tcttatcaag aatcccatct
     9421 cttgcttgct tttttttgtt gtcttccctt tgatagggtt tgtttttctt gtttcagtga
     9481 ctttctatgt taaaagataa tgtcagtaaa aggatttggt tttctattat tctgaatcga
     9541 ttacggaaga ttcttgctta attccaatct atacaagtat cgtgaaataa tgaccgttta
     9601 tgtgattagg agacgtgttt cattaataaa atataagatc aatacattgt tagtagtgat
     9661 aaactatgta caaattgtat tgattgtaaa agaaacacaa taggttcctt ttttctacaa
     9721 tatattgtga cagactctct gttttaacga atgaattaaa tttgtcaacg aaaaaattaa
     9781 agaatgatgt gatcactttg caccttggtt catcttcaga ggcttctgca tgttccatgc
     9841 attgaggctc tctacggtca caggttgtga gccgttgtta aagacaaaca aatgcgct
//
