LOCUS       sequence-435734-        9862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 pMUTE-Bxb1-tUBQ10, for stomata specific expression of Bxb1 
            integrase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9862)
  TITLE     Nemhauser Lab Plasmids 2024
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L1B39 - pMUTE-Bxb1-tUBQ10
FEATURES             Location/Qualifiers
     source          1..9862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    44..68
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(159..747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(239..258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2256..3071)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2256..3071)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2979..2998
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3063..3082
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3362..3797
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3924..3946
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    3954..4130
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4362..5387)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5407..5752)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5444..5464)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     5955..5977
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5969..5986
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5970..5986
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5993..6012
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5993..6011
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7721..9223
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISGAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
ORIGIN
        1 accacagaat caggggataa cgcaggaaag aacatgaagg ccttgacagg atatattggc
       61 gggtaaacta agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa aggccagcaa
      121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaagaag agttggtagc
      661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
      781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
      841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tactgagaga
      901 tcccctcata atttccccaa agcgtaacca tgtgtgaata aattttgagc tagtagggtt
      961 gcagccacga gtaagtcttc ccttgttatt gtgtagccag aatgccgcaa aacttccatg
     1021 cctaagcgaa ctgttgagag tacgtttcga tttctgactg tgttagcctg gaagtgcttg
     1081 tcccaacctt gtttctgagc atgaacgccc gcaagccaac atgttagttg aagcatcagg
     1141 gcgattagca gcatgatatc aaaacgctct gagctgctcg ttcggctatg gcgtaggcct
     1201 agtccgtagg caggactttt caagtctcgg aaggtttctt caatctgcat tcgcttcgaa
     1261 tagatattaa caagttgttt gggtgttcga atttcaacag gtaagttagt tgctagaacc
     1321 catggctcct ttgccgacgc tgagtagatt ttaggtgacg ggtggtgaca atgagtccgt
     1381 gtcgagcgct gattttttcg gcctttagag cgagatttat acaatagaat ttggcatgag
     1441 attggattgc ttttagtcag cctcttatag cctaaagtct ttgagtgact agatgacata
     1501 tcatgtaagt tgctgatagg tttccagttt tccgctccta ggtctgcata ttgtactttt
     1561 cctcttactc gacttaacca gtaccaaccc agcttctcaa cggatttata ccatggcact
     1621 ttaaagccag catcactgac aatgagcggt gtggtgttac tcggtagaat gctcgcaagg
     1681 tcggctagaa attggtcatg agctttcttt gaacattgct ctgaaagcgg gaacgctttc
     1741 tcataaagag taacagaacg accgtgtagt gcgactgaag ctcgcaatac cataagtcgt
     1801 ttttgctcac gaatatcaga ccagtcaaca agtacaatgg gcatcgtatt gcccgaacag
     1861 ataaagctag catgccaacg gtatacagcg agtcgctctt tgtggaggtg acgattacct
     1921 aacaatcggt cgattcgttt gatgttatgt tttgttctcg ctttggttgg caggttacgg
     1981 ccaagttcgg taagagtgag agttttacag tcaagtaatg cgtggcaagc caacgttaag
     2041 ctgttgagtc gttttaagtg taattcgggg cagaattggt aaagagagtc gtgtaaaata
     2101 tcgagttcgc acatcttgtt gtctgattat tgatttttcg cgaaaccatt tgatcatatg
     2161 acaagatgtg tatccacctt aacttaatga tttttaccaa aatcattagg ggattcatca
     2221 gtctaaagta tatatgtgta acattggtct agtgattaga aaaactcatc gagcatcaaa
     2281 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     2341 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     2401 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     2461 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     2521 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     2581 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     2641 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     2701 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     2761 ttccctggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     2821 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     2881 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     2941 tacaatcggt agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     3001 tataaatcag catccatgtt ggaatttaat cgcggccttg agcaagacgt ttcccgttga
     3061 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     3121 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     3181 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac
     3241 gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa
     3301 gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat tcaggcgatc
     3361 cccatccaac agcccgccgt cgagcgggct tttttatccc cggaagcctg tggatagagg
     3421 gtagttatcc acgtgaaacc gctaatgccc cgcaaagcct tgattcacgg ggctttccgg
     3481 cccgctccaa aaactatcca cgtgaaatcg ctaatcaggg tacgtgaaat cgctaatcgg
     3541 agtacgtgaa atcgctaata aggtcacgtg aaatcgctaa tcaaaaaggc acgtgagaac
     3601 gctaatagcc ctttcagatc aacagcttgc aaacacccct cgctccggca agtagttaca
     3661 gcaagtagta tgttcaatta gcttttcaat tatgaatata tatatcaatt attggtcgcc
     3721 cttggcttgt ggacaatgcg ctacgcgcac cggctccgcc cgtggacaac cgcaagcggt
     3781 tgcccaccgt cgagcgcctt tgcccacaac ccggcggccg gccgcaacag atcgttttat
     3841 aaattttttt ttttgaaaaa gaaaaagccc gaaaggcggc aacctctcgg gcttctggat
     3901 ttccgatccc cggaattaga tcttggcagg atatattgtg gtgtaacgtt atcgatctgg
     3961 attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca
     4021 aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa ccctataaga
     4081 accctaattt cccttatcgg gaaactactc acacattatt tatggagaaa atagagagag
     4141 atagatttgt agagagagac tggtgatttc agcgtaccga attaattcct gaatcactgc
     4201 gaccggccct cccgcgaccc agccgagcga gcttagcgaa ctgtggacga gaactgtgcc
     4261 accaagcgta aggccgttct ctcgcatttg ccttgctagg ctcgcgcgag ttgctggctg
     4321 aggcgttctc gaaatcagct cttgttcggt cggcatctac tctattcctt tgccctcgga
     4381 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
     4441 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
     4501 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
     4561 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccttgg cgatcctgca
     4621 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
     4681 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
     4741 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
     4801 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
     4861 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
     4921 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
     4981 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatagcatc catagcctcc
     5041 gcgaccggct gaagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
     5101 tgtgcacggc gggagatgca ataggtcagg ctctcgctga actccccaat gtcaagcact
     5161 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
     5221 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
     5281 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
     5341 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatagg ggggatcagc
     5401 ttgggctgtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
     5461 atagtgggat tgtgcgtcat cccttacgtc agtggagatg tcacatcaat ccacttgctt
     5521 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     5581 tttgggacca ctgtcggcag aggcatcttg aatgatagcc tttcctttat cgcaatgatg
     5641 gcatttgtag gagccacctt ccttttctac tgtcctttcg atgaagtgac agatagctgg
     5701 gcaatggaat ccgaggaggt ttcccgaaat taccctttgt tgaaaagtct caatagccct
     5761 ttggtcttct gagactgtat ctttgacatt tttggagtag gggtacgata acattaacgc
     5821 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5881 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     5941 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     6001 ctcactatag ggcttggagc caaaccaatc ccatacccaa accagactga aaattggttg
     6061 gtttggtttt cgacccttgc attgaatgtt aaccgatatc ttgatcgaac gtgagttttt
     6121 tttttttttt ttcttgtttc attgccttgt cgatcaatat tgtatataac gtgatagacc
     6181 atgaacaaat tgaatatcga agtaggagtt agatttcttt actcgtaatt aaatggggca
     6241 tgcaccttgc atgtcttata cgtattgtag cctaacattt gcggctacaa acatttgtca
     6301 ccaacatttt cattccagct gcttccacag ttttgggact tcggagataa tgtatatgtg
     6361 gatatgcata tatgaaccat aaaaataaac gtttgtccaa acaaaaccac aaatagaagt
     6421 gttatccaag tgggtttgtt tcatagaaaa aatattgtca ctatagcaat ttttttggtt
     6481 aggttaaacc ttatttaatg aagtttgtaa tatgaattga gatattgtta actttgcaaa
     6541 acagagccga aattttacga atatctcttt attaaaaata aataatataa tttaagaata
     6601 cttctcggga ggaatcttaa tgagccgtcc aggtagctga acaactgaac tttatctttg
     6661 ctccaaaggt tatatcaatt tatgttttct tgtagaacct atatttcatg caaaaagtta
     6721 tccattaacc aatggaaatt ggttccgact agtgatcaag gaaaatgatg gaatcatcac
     6781 gaatgagaaa tttgtggtat taaatgacat agtgattaag gaaaacaaaa agttgtaatt
     6841 caactacaaa ctatgtcgta actatttata ttttgttact tgtcaatgtt gtcatttatc
     6901 ttacaaagtc aataggatag aagatgtgaa acataattac acaaaaacga tgtcatatag
     6961 aaacatgcga aatcacataa tcatttcatc acttcgaaat ttcgtagatt tccaatcaga
     7021 tactacccaa tatgtttgct aatagtaagt agtaaaaaac atatgttacg ccaaaaaaaa
     7081 cggcttttga tgtacttatt ttccttcatc gatccatgaa aactgtaaaa catctcaata
     7141 caaactaaac ctttccacca gtatatgtcc gattcaatgt ttttttactt tgtgtatgta
     7201 tcagtgtatt atctgttaat aaagagtaaa agaaatccat tttttcttgt gaggacacgc
     7261 ggtaattaag aacaaagaag tggcacaagt gtagaaatta aactagaagt aaaagaaggt
     7321 ggtgcaaagg catgcactct aaatgaaaat acgatattga aattcataaa agacctcacg
     7381 cagtgaatgt aaatgtaaat gtatccaact tcacaaacgt gacgtatctt tgtgtatgca
     7441 tacctttttc tttacgttgt tctaatacat atcgatagta ataagagaga gaaagagaaa
     7501 aatcaagaag agacaaaaga atgtcacgag acagcgacat agtgcattta tgaaccttga
     7561 aaagaaattc taaaccctaa ttatcacttg ttgttgcggc tgtataaata cgactttgtt
     7621 ttgaagaatg tgtcgaacaa agtgaaaaca taagatcatc ttcttcgttg atagatcaat
     7681 ataggaactc cagaagagaa tcttgatcaa ttaagtatca atgagagccc tggtagtcat
     7741 ccgcctgtcc cgcgtcaccg atgctacgac ttcaccggag cgtcagctgg agtcttgcca
     7801 gcagctctgc gcccagcgcg gctgggacgt cgtcggggta gcggaggatc tggacgtctc
     7861 cggggcggtc gatccgttcg accggaagcg cagaccgaac ctggcccggt ggctagcgtt
     7921 cgaggagcaa ccgtttgacg tgatcgtggc gtaccgggta gatcggttga cccgatcgat
     7981 ccggcatctt cagcagctgg tccactgggc cgaggaccac aagaagctgg tcgtctccgc
     8041 gaccgaagcg cacttcgata cgacgacgcc gtttgcggcg gtcgtcatcg cgcttatggg
     8101 aacggtggcg cagatggaat tagaagcgat caaagagcgg aaccgttcgg ctgcgcattt
     8161 caatatccgc gccgggaaat accgaggatc cctgccgccg tggggatacc tgcctacgcg
     8221 cgtggacggg gagtggcggc tggtgccgga ccctgtgcag cgagagcgca tcctcgaggt
     8281 gtatcaccgc gtcgtcgaca accacgagcc gctgcatctg gtggcccacg acctgaaccg
     8341 gcgtggtgtc ctgtcgccga aggactactt cgcgcagctg caaggccgcg agccgcaggg
     8401 ccgggagtgg tcggctaccg cgctgaagcg atcgatgatc tccggggcga tgctcgggta
     8461 cgcgactctg aacggtaaga ccgtccgaga cgacgacgga gccccgctgg tgcgggctga
     8521 gccgatcctg acccgtgagc agctggaggc gctgcgcgcc gagctcgtga agacctcccg
     8581 ggcgaagccc gcggtgtcta ccccgtcgct gctgctgcgg gtgttgttct gcgcggtgtg
     8641 cggggagccc gcgtacaagt tcgccggggg aggacgtaag cacccgcgct accgctgccg
     8701 ctcgatgggg ttcccgaagc actgcgggaa cggcacggtg gcgatggccg agtgggacgc
     8761 gttctgcgag gagcaggtac tggatctgct cggggacgcg gagcgtctgg agaaagtctg
     8821 ggtagcgggc tcggactccg cggtcgaact cgcggaggtg aacgcggagc tggtggacct
     8881 gacgtcgctg atcggctccc cggcctaccg ggcgggctct ccgcagcgag aagcactgga
     8941 tgcccgtatt gcggcgctgg ccgcgcggca agaggagctg gagggcctgg aggctcgccc
     9001 gtctggctgg gagtggcgcg aaaccgggca gcggttcggg gactggtggc gggagcagga
     9061 caccgcggca aagaacacct ggcttcggtc gatgaacgtt cggctgacgt tcgacgtccg
     9121 cggcgggctg actcgcacga tcgacttcgg ggatcttcag gagtacgagc agcatctcag
     9181 gctcggcagc gtggtcgaac ggctacacac cgggatgtcg taataagctt atctcgtctc
     9241 tgttatgctt aagaagttca atgtttcgtt tcatgtaaaa ctttggtggt ttgtgttttg
     9301 gggccttgta taatccctga tgaataagtg ttctactatg tttccgttcc tgttatctct
     9361 ttctttctaa tgacaagtcg aacttcttct ttatcatcgc ttcgttttta ttatctgtgc
     9421 ttcttttgtt taatacgcct gcaaagtgac tcgactctgt ttagtgcagt tctgcgaaac
     9481 ttgtaaatag tccaattgtt ggcctctagt aatagatgta gcgaaagtgt tgagctgttg
     9541 ggttctaagg atggcttgaa catgttaatc ttttaggttc tgagtatgat gaacattcgt
     9601 tgttgctaag aaatgcctgt aatgtcccac aaatgtagaa aatggttcgt acctttgtcc
     9661 aagcattgat atgtctgatg agaggaaact gcaagatact gagcttggtt taacgaagga
     9721 gaggcagttt cttccttcca aagcatttca tttgacaatg ccttgatcat cttaagtaga
     9781 gtttctgttg tggaaagttt gaaactttga agaaacgact ctcaagtaaa ttgatgatca
     9841 caagtgaaag tgtatgttcg ct
//
