LOCUS       sequence-435763-        4179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Dong G, Nakai T, Matsuzaki T
  TITLE     A novel plasmid-based experimental system in Saccharomyces 
            cerevisiae that enables the introduction of 10 different plasmids 
            into cells.
  JOURNAL   FEBS Open Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893.
  PUBMED    39387713
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Open 
            Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893."
COMMENT     Sequence Label: pESC-LYS
FEATURES             Location/Qualifiers
     source          1..4179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..4179
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
ORIGIN
        1 atgactaacg aaaaggtctg gatagagaag ttggataatc caactctttc agtgttacca
       61 catgactttt tacgcccaca acaagaacct tatacgaaac aagctacata ttcgttacag
      121 ctacctcagc tcgatgtgcc tcatgatagt ttttctaaca aatacgctgt cgctttgagt
      181 gtatgggctg cattgatata tagagtaacc ggtgacgatg atattgttct ttatattgcg
      241 aataacaaaa tcttaagatt caatattcaa ccaacgtggt catttaatga gctgtattct
      301 acaattaaca atgagttgaa caagctcaat tctattgagg ccaatttttc ctttgacgag
      361 ctagctgaaa aaattcaaag ttgccaagac ctggaaagga cccctcagtt gttccgtttg
      421 gcctttttgg aaaaccaaga tttcaaatta gacgagttca agcatcattt agtggacttt
      481 gctttgaatt tggataccag taataatgcg catgttttga acttaattta taacagctta
      541 ctgtattcga atgaaagagt aaccattgtt gcggaccaat ttactcaata tttgactgct
      601 gcgctaagcg atccatccaa ttgcataact aaaatctctc tgatcaccgc atcatccaag
      661 gatagtttac ctgatccaac taagaacttg ggctggtgcg atttcgtggg gtgtattcac
      721 gacattttcc aggacaatgc tgaagccttc ccagagagaa cctgtgttgt ggagactcca
      781 acactaaatt ccgacaagtc ccgttctttc acttatcgcg acatcaaccg cacttctaac
      841 atagttgccc attatttgat taaaacaggt atcaaaagag gtgatgtagt gatgatctat
      901 tcttctaggg gtgtggattt gatggtatgt gtgatgggtg tcttgaaagc cggcgcaacc
      961 ttttcagtta tcgaccctgc atatccccca gccagacaaa ccatttactt aggtgttgct
     1021 aaaccacgtg ggttgattgt tattagagct gctggacaat tggatcaact agtagaagat
     1081 tacatcaatg atgaattgga gattgtttca agaatcaatt ccatcgctat tcaagaaaat
     1141 ggtaccattg aaggtggcaa attggacaat ggcgaggatg ttttggctcc atatgatcac
     1201 tacaaagaca ccagaacagg tgttgtagtt ggaccagatt ccaacccaac cctatctttc
     1261 acatctggtt ccgaaggtat tcctaagggt gttcttggta gacatttttc cttggcttat
     1321 tatttcaatt ggatgtccaa aaggttcaac ttaacagaaa atgataaatt cacaatgctg
     1381 agcggtattg cacatgatcc aattcaaaga gatatgttta caccattatt tttaggtgcc
     1441 caattgtatg tccctactca agatgatatt ggtacaccgg gccgtttagc ggaatggatg
     1501 agtaagtatg gttgcacagt tacccattta acacctgcca tgggtcaatt acttactgcc
     1561 caagctacta caccattccc taagttacat catgcgttct ttgtgggtga cattttaaca
     1621 aaacgtgatt gtctgaggtt acaaaccttg gcagaaaatt gccgtattgt taatatgtac
     1681 ggtaccactg aaacacagcg tgcagtttct tatttcgaag ttaaatcaaa aaatgacgat
     1741 ccaaactttt tgaaaaaatt gaaagatgtc atgcctgctg gtaaaggtat gttgaacgtt
     1801 cagctactag ttgttaacag gaacgatcgt actcaaatat gtggtattgg cgaaataggt
     1861 gagatttatg ttcgtgcagg tggtttggcc gaaggttata gaggattacc agaattgaat
     1921 aaagaaaaat ttgtgaacaa ctggtttgtt gaaaaagatc actggaatta tttggataag
     1981 gataatggtg aaccttggag acaattctgg ttaggtccaa gagatagatt gtacagaacg
     2041 ggtgatttag gtcgttatct accaaacggt gactgtgaat gttgcggtag ggctgatgat
     2101 caagttaaaa ttcgtgggtt cagaatcgaa ttaggagaaa tagatacgca catttcccaa
     2161 catccattgg taagagaaaa cattacttta gttcgcaaaa atgccgacaa tgagccaaca
     2221 ttgatcacat ttatggtccc aagatttgac aagccagatg acttgtctaa gttccaaagt
     2281 gatgttccaa aggaggttga aactgaccct atagttaagg gcttaatcgg ttaccatctt
     2341 ttatccaagg acatcaggac tttcttaaag aaaagattgg ctagctatgc tatgccttcc
     2401 ttgattgtgg ttatggataa actaccattg aatccaaatg gtaaagttga taagcctaaa
     2461 cttcaattcc caactcccaa gcaattaaat ttggtagctg aaaatacagt ttctgaaact
     2521 gacgactctc agtttaccaa tgttgagcgc gaggttagag acttatggtt aagtatatta
     2581 cctaccaagc cagcatctgt atcaccagat gattcgtttt tcgatttagg tggtcattct
     2641 atcttggcta ccaaaatgat ttttacctta aagaaaaagc tgcaagttga tttaccattg
     2701 ggcacaattt tcaagtatcc aacgataaag gcctttgccg cggaaattga cagaattaaa
     2761 tcatcgggtg gatcatctca aggtgaggtc gtcgaaaatg tcactgcaaa ttatgcggaa
     2821 gacgccaaga aattggttga gacgctacca agttcgtacc cctctcgtga atattttgtt
     2881 gaacctaata gtgccgaagg aaaaacaaca attaatgtgt ttgttaccgg tgtcacagga
     2941 tttctgggct cctacatcct tgcagatttg ttaggacgtt ctccaaagaa ctacagtttc
     3001 aaagtgtttg cccacgtcag ggccaaggat gaagaagctg catttgcaag attacaaaag
     3061 gcaggtatca cctatggtac ttggaacgaa aaatttgcct caaatattaa agttgtatta
     3121 ggcgatttat ctaaaagcca atttggtctt tcagatgaga agtggatgga tttggcaaac
     3181 acagttgata taattatcca taatggtgcg ttagttcact gggtttatcc atatgccaaa
     3241 ttgagggacc caaatgttat ttcaactatc aatgttatga gcttagccgc cgtcggcaag
     3301 ccaaagttct ttgactttgt ttcctccact tctactcttg acactgaata ctactttaat
     3361 ttgtcagata aacttgttag cgaagggaag ccaggcattt tagaatcaga cgatttaatg
     3421 aactctgcaa gcgggctcac tggtggatat ggtcagtcca aatgggctgc tgagtacatc
     3481 attagacgtg caggtgaaag gggcctacgt gggtgtattg tcagaccagg ttacgtaaca
     3541 ggtgcctctg ccaatggttc ttcaaacaca gatgatttct tattgagatt tttgaaaggt
     3601 tcagtccaat taggtaagat tccagatatc gaaaattccg tgaatatggt tccagtagat
     3661 catgttgctc gtgttgttgt tgctacgtct ttgaatcctc ccaaagaaaa tgaattggcc
     3721 gttgctcaag taacgggtca cccaagaata ttattcaaag actacttgta tactttacac
     3781 gattatggtt acgatgtcga aatcgaaagc tattctaaat ggaagaaatc attggaggcg
     3841 tctgttattg acaggaatga agaaaatgcg ttgtatcctt tgctacacat ggtcttagac
     3901 aacttacctg aaagtaccaa agctccggaa ctagacgata ggaacgccgt ggcatcttta
     3961 aagaaagaca ccgcatggac aggtgttgat tggtctaatg gaataggtgt tactccagaa
     4021 gaggttggta tatatattgc atttttaaac aaggttggat ttttacctcc accaactcat
     4081 aatgacaaac ttccactgcc aagtatagaa ctaactcaag cgcaaataag tctagttgct
     4141 tcaggtgctg gtgctcgtgg aagctccgca gcagcttaa
//
