LOCUS       sequence-435769-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of two genes in yeast with V5 and HA tags and LEU2 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Dong G, Nakai T, Matsuzaki T
  TITLE     A novel plasmid-based experimental system in Saccharomyces 
            cerevisiae that enables the introduction of 10 different plasmids 
            into cells.
  JOURNAL   FEBS Open Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893.
  PUBMED    39387713
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Open 
            Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893."
COMMENT     Sequence Label: pESC-LEU (V5 and HA)
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1095
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc acgttggtca agaaatcaca
       61 gccgaagcca ttaaggttct taaagctatt tctgatgttc gttccaatgt caagttcgat
      121 ttcgaaaatc atttaattgg tggtgctgct atcgatgcta caggtgtccc acttccagat
      181 gaggcgctgg aagcctccaa gaaggttgat gccgttttgt taggtgctgt ggctggtcct
      241 aaatggggta ccggtagtgt tagacctgaa caaggtttac taaaaatccg taaagaactt
      301 caattgtacg ccaacttaag accatgtaac tttgcatccg actctctttt agacttatct
      361 ccaatcaagc cacaatttgc taaaggtact gacttcgttg ttgtcagaga attagtggga
      421 ggtatttact ttggtaagag aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa
      481 caatacaccg ttccagaagt gcaaagaatc acaagaatgg ccgctttcat ggccctacaa
      541 catgagccac cattgcctat ttggtccttg gataaagcta atcttttggc ctcttcaaga
      601 ttatggagaa aaactgtgga ggaaaccatc aagaacgaat tccctacatt gaaggttcaa
      661 catcaattga ttgattctgc cgccatgatc ctagttaaga acccaaccca cctaaatggt
      721 attataatca ccagcaacat gtttggtgat atcatctccg atgaagcctc cgttatccca
      781 ggttccttgg gtttgttgcc atctgcgtcc ttggcctctt tgccagacaa gaacaccgca
      841 tttggtttgt acgaaccatg ccacggttct gctccagatt tgccaaagaa taaggttgac
      901 cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa
      961 gaaggtaagg ccattgaaga tgcagttaaa aaggttttgg atgcaggtat cagaactggt
     1021 gatttaggtg gttccaacag taccaccgaa gtcggtgatg ctgtcgccga agaagttaag
     1081 aaaatccttg cttaa
//
