LOCUS       sequence-43577-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA4c-RFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      149..220
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      236..263
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(291..308)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(505..524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
       61 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaaggat
      121 ccaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
      181 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      241 accttcgggt gggcctttct gcgtttatac ctagggatat attccgcttc ctcgctcact
      301 gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga
      361 gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc
      421 cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt
      481 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg
      541 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt
      601 tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt
      661 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      721 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
      781 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
      841 ctgcgctcct
//
