LOCUS       sequence-435771-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of two genes in yeast with V5 and HA tags and HIS3 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Dong G, Nakai T, Matsuzaki T
  TITLE     A novel plasmid-based experimental system in Saccharomyces 
            cerevisiae that enables the introduction of 10 different plasmids 
            into cells.
  JOURNAL   FEBS Open Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893.
  PUBMED    39387713
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Open 
            Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893."
COMMENT     Sequence Label: pESC-HIS (V5 and HA)
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..660
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 atgacagagc agaaagccct agtaaagcgt attacaaatg aaaccaagat tcagattgcg
       61 atctctttaa agggtggtcc cctagcgata gagcactcga tcttcccaga aaaagaggca
      121 gaagcagtag cagaacaggc cacacaatcg caagtgatta acgtccacac aggtataggg
      181 tttctggacc atatgataca tgctctggcc aagcattccg gctggtcgct aatcgttgag
      241 tgcattggtg acttacacat agacgaccat cacaccactg aagactgcgg gattgctctc
      301 ggtcaagctt ttaaagaggc cctactggcg cgtggagtaa aaaggtttgg atcaggattt
      361 gcgcctttgg atgaggcact ttccagagcg gtggtagatc tttcgaacag gccgtacgca
      421 gttgtcgaac ttggtttgca aagggagaaa gtaggagatc tctcttgcga gatgatcccg
      481 cattttcttg aaagctttgc agaggctagc agaattaccc tccacgttga ttgtctgcga
      541 ggcaagaatg atcatcaccg tagtgagagt gcgttcaagg ctcttgcggt tgccataaga
      601 gaagccacct cgcccaatgg taccaacgat gttccctcca ccaaaggtgt tcttatgtag
//
