LOCUS       sequence-435773-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of two genes in yeast with V5 and HA tags and TRP1 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Dong G, Nakai T, Matsuzaki T
  TITLE     A novel plasmid-based experimental system in Saccharomyces 
            cerevisiae that enables the introduction of 10 different plasmids 
            into cells.
  JOURNAL   FEBS Open Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893.
  PUBMED    39387713
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Open 
            Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893."
COMMENT     Sequence Label: pESC-TRP (V5 and HA)
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..675
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 atgtctgtta ttaatttcac aggtagttct ggtccattgg tgaaagtttg cggcttgcag
       61 agcacagagg ccgcagaatg tgctctagat tccgatgctg acttgctggg tattatatgt
      121 gtgcccaata gaaagagaac aattgacccg gttattgcaa ggaaaatttc aagtcttgta
      181 aaagcatata aaaatagttc aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa
      241 cctaaggagg atgttttggc tctggtcaat gattacggca ttgatatcgt ccaactgcat
      301 ggagatgagt cgtggcaaga ataccaagag ttcctcggtt tgccagttat taaaagactc
      361 gtatttccaa aagactgcaa catactactc agtgcagctt cacagaaacc tcattcgttt
      421 attcccttgt ttgattcaga agcaggtggg acaggtgaac ttttggattg gaactcgatt
      481 tctgactggg ttggaaggca agagagcccc gaaagcttac attttatgtt agctggtgga
      541 ctgacgccag aaaatgttgg tgatgcgctt agattaaatg gcgttattgg tgttgatgta
      601 agcggaggtg tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa
      661 aatgctaaga aatag
//
