LOCUS       sequence-435777-        1335 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of two genes in yeast with V5 and HA tags and MET15 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Dong G, Nakai T, Matsuzaki T
  TITLE     A novel plasmid-based experimental system in Saccharomyces 
            cerevisiae that enables the introduction of 10 different plasmids 
            into cells.
  JOURNAL   FEBS Open Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893.
  PUBMED    39387713
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Open 
            Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893."
COMMENT     Sequence Label: pESC-MET (V5 and HA)
FEATURES             Location/Qualifiers
     source          1..1335
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1335
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
ORIGIN
        1 atgccatctc atttcgatac tgttcaacta cacgccggcc aagagaaccc tggtgacaat
       61 gctcacagat ccagagctgt accaatttac gccaccactt cttatgtttt cgaaaactct
      121 aagcatggtt cgcaattgtt tggtctagaa gttccaggtt acgtctattc ccgtttccaa
      181 aacccaacca gtaatgtttt ggaagaaaga attgctgctt tagaaggtgg tgctgctgct
      241 ttggctgttt cctccggtca agccgctcaa acccttgcca tccaaggttt ggcacacact
      301 ggtgacaaca tcgtttccac ttcttactta tacggtggta cttataacca gttcaaaatc
      361 tcgttcaaaa gatttggtat cgaggctaga tttgttgaag gtgacaatcc agaagaattt
      421 gaaaaggtct ttgatgaaag aaccaaggct gtttatttgg aaaccattgg taatccaaag
      481 tacaatgttc cggattttga aaaaattgtt gcaattgctc acaaacacgg tattccagtt
      541 gtcgttgaca acacatttgg tgccggtggt tacttctgtc agccaattaa atacggtgct
      601 gatattgtaa cacattctgc taccaaatgg attggtggtc atggtactac tatcggtggt
      661 attattgttg actctggtaa gttcccatgg aaggactacc cagaaaagtt ccctcaattc
      721 tctcaacctg ccgaaggata tcacggtact atctacaatg aagcctacgg taacttggca
      781 tacatcgttc atgttagaac tgaactatta agagatttgg gtccattgat gaacccattt
      841 gcctctttct tgctactaca aggtgttgaa acattatctt tgagagctga aagacacggt
      901 gaaaatgcat tgaagttagc caaatggtta gaacaatccc catacgtatc ttgggtttca
      961 taccctggtt tagcatctca ttctcatcat gaaaatgcta agaagtatct atctaacggt
     1021 ttcggtggtg tcttatcttt cggtgtaaaa gacttaccaa atgccgacaa ggaaactgac
     1081 ccattcaaac tttctggtgc tcaagttgtt gacaatttaa agcttgcctc taacttggcc
     1141 aatgttggtg atgccaagac cttagtcatt gctccatact tcactaccca caaacaatta
     1201 aatgacaaag aaaagttggc atctggtgtt accaaggact taattcgtgt ctctgttggt
     1261 atcgaattta ttgatgacat tattgcagac ttccagcaat cttttgaaac tgttttcgct
     1321 ggccaaaaac catga
//
