LOCUS       sequence-435781-        1359 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of two genes in yeast with V5 and HA tags and TYR1 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Dong G, Nakai T, Matsuzaki T
  TITLE     A novel plasmid-based experimental system in Saccharomyces 
            cerevisiae that enables the introduction of 10 different plasmids 
            into cells.
  JOURNAL   FEBS Open Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893.
  PUBMED    39387713
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Open 
            Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893."
COMMENT     Sequence Label: pESC-TYR (V5 and HA)
FEATURES             Location/Qualifiers
     source          1..1359
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtatcag aggataagat tgagcaatgg aaagccacaa aagtcattgg tataattggt
       61 ctgggtgata tgggcctatt atacgctaat aaatttacag atgctggatg gggtgttata
      121 tgttgtgata gggaagaata ttatgatgaa ctgaaagaaa aatatgcctc agctaaattc
      181 gaactggtga aaaatggtca tttggtatcc aggcaaagcg actatattat ctatagtgtt
      241 gaagcatcca atattagtaa gatcgtcgca acgtatggac catcttctaa ggttggaaca
      301 attgttgggg gtcaaacgag ttgtaagctg ccggaaatcg aggctttcga aaagtattta
      361 cccaaggact gcgacatcat taccgtgcat tcccttcatg ggcctaaagt taatactgaa
      421 ggccaaccac ttgttattat caatcacaga tcacagtacc cagaatcttt tgagttcgtt
      481 aattctgtta tggcatgttt gaaaagtaag caagtttatt tgacatatga agagcatgac
      541 aagattaccg ctgatacaca agctgtgaca catgctgctt tcttaagtat gggatctgcg
      601 tgggcaaaga taaagattta tccttggact ctgggtgtaa acaaatggta cggtggccta
      661 gaaaatgtga aagttaatat atcactaaga atctattcga acaagtggca tgtttacgca
      721 ggattagcca taacaaaccc aagtgcacat cagcaaattc ttcaatatgc aaccagtgca
      781 acagaactat ttagtttaat gatagataac aaagaacaag aacttactga tagactatta
      841 aaagctaagc aatttgtatt tggaaagcat actggtctct tactattgga tgacacgatt
      901 ttagagaaat attcgctatc aaaaagcagc attggtaaca gcaacaattg caagccagtg
      961 ccgaactcac atttatcatt gttggcgatt gttgattcgt ggtttcaact tggtattgat
     1021 ccatatgatc atatgatttg ttcgacgcca ttattcagaa tattcctggg tgtgtccgaa
     1081 tatctttttt taaaacctgg cttattagaa cagacaattg atgcagctat ccatgataaa
     1141 tcattcataa aagatgattt agaatttgtt atttcggcta gagaatggag ttcggttgtt
     1201 tcttttgcca attttgatat atacaaaaag caatttcaga gtgttcaaaa gttctttgag
     1261 ccaatgcttc cagaggctaa tctcattggc aacgagatga taaaaaccat tctgagtcat
     1321 tctagtgacc gttcggccgc tgaaaaaaga aatacataa
//
