LOCUS       sequence-435785-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of two genes in yeast with V5 and HA tags and THR1 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Dong G, Nakai T, Matsuzaki T
  TITLE     A novel plasmid-based experimental system in Saccharomyces 
            cerevisiae that enables the introduction of 10 different plasmids 
            into cells.
  JOURNAL   FEBS Open Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893.
  PUBMED    39387713
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Open 
            Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893."
COMMENT     Sequence Label: pESC-THR (V5 and HA)
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggttcgtg ccttcaaaat taaagttcca gcttcttccg ccaatatcgg ccctggttat
       61 gatgttcttg gtgtcggtct ttctctattc ttggagttag atgtcaccat cgactccagc
      121 caagctcagg aaacaaacga cgatcccaac aactgcaagc tgtcttacac taaagaaagt
      181 gaaggctatt ctacggtccc attgcgttct gatgctaacc tgattaccag gactgcgtta
      241 tatgtgttgc gttgcaacaa tattagaaac ttcccctccg gaaccaaagt tcacgtctcc
      301 aacccaatcc cacttggccg tggattgggt tcctctggtg cagcagttgt ggcaggtgtt
      361 attttaggta acgaagtggc ccaattgggt ttctctaagc aacgtatgtt ggactactgt
      421 ttgatgattg aacgtcatcc agacaacata accgctgcta tgatgggagg cttttgcggt
      481 tcattcctaa gggacttgac cccacaagag gtggaaagac gtgagattcc attggctgag
      541 gtgcttccag aaccttctgg tggtgaagat accggtctgg ttcccccatt acctcccacc
      601 gatatcggta gacatgtcaa ataccaatgg aaccccgcca ttaaatgtat tgcgatcatc
      661 ccacagttcg agttgtccac cgccgactcc agaggcgttc ttccaaaagc ctacccaacc
      721 caggacttgg ttttcaatct acaaagattg gccgtcttga ccacagcttt gaccatggac
      781 ccacctaatg ccgacttaat ctaccctgct atgcaagatc gtgtccacca accttataga
      841 aagacattga tcccaggtct cacggaaatc ttatcatgtg tcaccccatc cacataccct
      901 ggcctattgg gtatctgctt gtcaggtgca ggcccaacta tcttggcttt ggccactgag
      961 aatttcgaag aaatctctca agaaattatc aacaggttcg ccaaaaacgg catcaagtgc
     1021 tcctggaaac tactggagcc tgcctacgat ggtgcaagcg tcgaacagca atga
//
