LOCUS       sequence-43586-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS4k-RFP
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      149..220
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      236..263
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
       61 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaaggat
      121 ccaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
      181 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      241 accttcgggt gggcctttct gcgtttatac ctagggtacg ggttttgctg cccgcaaacg
      301 ggctgttctg gtgttgctag tttgttatca gaatcgcaga tccggcttca gccggtttgc
      361 cggctgaaag cgctatttct tccagaattg ccatgatttt ttccccacgg gaggcgtcac
      421 tggctcccgt gttgtcggca gctttgattc gataagcagc atcgcctgtt tcaggctgtc
      481 tatgtgtgac tgttgagctg taacaagttg tctcaggtgt tcaatttcat gttctagttg
      541 ctttgtttta ctggtttcac ctgttctatt aggtgttaca tgctgttcat ctgttacatt
      601 gtcgatctgt tcatggtgaa cagctttgaa tgcaccaaaa actcgtaaaa gctctgatgt
      661 atctatcttt tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatgta
      721 acggtgaaca gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag
      781 agccataaga acctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt
      841 gtttttgcgt gagccatg
//
