LOCUS       sequence-435865-       14303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of GFP-tagged SETX construct for subcellular 
            localization studies with GFP, or immunoprecipitation with Flag 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14303)
  AUTHORS   Bennett CL, Sopher BL, La Spada AR
  TITLE     Tight expression regulation of senataxin, linked to motor neuron 
            disease and ataxia, is required to avert cell-cycle block and 
            nucleolus disassembly.
  JOURNAL   Heliyon. 2020 Jun 13;6(6):e04165. doi: 
            10.1016/j.heliyon.2020.e04165. eCollection 2020 Jun.
  PUBMED    32577562
REFERENCE   2  (bases 1 to 14303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Heliyon."; 
            date: "2020-06-13"; volume: "6(6):e04165. doi"; pages: " 
            10.1016/j.heliyon.2020.e04165. eCollection 2020 Jun"
COMMENT     Sequence Label: 3xFlag-eGFP-Flag-SETX
FEATURES             Location/Qualifiers
     source          1..14303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..31
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(250..354)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(429..448)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        689..992
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        993..1196
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1147..1167
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1241..1260
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1241..1259
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1279..1288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1288..1353
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(1366..1368,1369..1371,1372..2082)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1366..1368,1369..1371,1372..2082)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1411..1432)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1672..1691)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2019..2040
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2137..2160
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10202..10225
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10237..10254
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10277..10294)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10283..10507
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10553..10981
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10640..10659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10850..10871
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10990..11010)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10995..11324
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11175..11310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11237..11256
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             11391..12185
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11445..11464)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12055..12074
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    12361..12482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12398..12417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12452..12471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12531..12547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12531..12547)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12544..12566)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12555..12571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12579..12585,12586..12603,12604..12609))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12624..12645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12762..12779)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12933..13521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13013..13032)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13692..14303,1..180,181..249))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
       61 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      361 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
      421 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
      481 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
      541 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
      601 cgggccagat atacgcgccg gacgcgtcta gttattaata gtaatcaatt acggggtcat
      661 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      721 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      781 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      841 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      901 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      961 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     1021 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1081 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     1141 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     1201 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     1261 agacccaagc tggctagcgc caccatggac tacaaagacc atgacggcga ttataaagat
     1321 catgacattg attacaagga tgacgatgac aagctggcga tcggaatggt gagcaagggc
     1381 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1441 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1501 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1561 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1621 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1681 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1741 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1801 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1861 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1921 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1981 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2041 accgccgccg ggatcactct cggcatggac gagctgtaca agggtaccga gctcggatcc
     2101 actagtccag tgtggtggaa ttgcccttcc accatggatt acaaggatga cgacgataag
     2161 ggtggcagca catgttgttg gtgtacgcca ggtggtgctt ccaccattga cttcctaaag
     2221 cgctatgctt ccaacactcc gtccggtgaa tttcaaacag ccgacgaaga cctctgctac
     2281 tgcttggagt gtgtggctga gtaccacaaa gcaagagatg aattgccatt cttgcatgag
     2341 gttttatggg aattagaaac cttacgtctc ataaatcact ttgaaaaatc catgaaggca
     2401 gaaattggag atgatgatga gttatatata gtagacaata atggagagat gccactgttt
     2461 gacatcactg ggcaagactt tgaaaataag cttcgagttc ctcttcttga aatactgaaa
     2521 tatccttact tgcttctaca tgaacgtgtt aacgagttat gtgttgaagc actttgtcgg
     2581 atggaacaag ccaattgctc ctttcaggtg tttgataaac atccagggat ctatttgttt
     2641 ttagtccatc ccaatgaaat ggttcggcgt tgggctatct tgactgcaag aaacttgggg
     2701 aaagtggaca gagatgatta ttatgactta caagaagttt tactttgcct ttttaaagtc
     2761 attgagttgg ggcttttaga gagtccagac atttatactt cttctgtcct agagaagggt
     2821 aaactgattc ttctgccctc acacatgtat gatactacca actacaaaag ctattggtta
     2881 ggtatttgca tgttgctgac cattcttgag gaacaagcca tggattccct gttgttgggc
     2941 tcagacaaac aaaatgattt tatgcaatcg atacttcaca ctatggagag ggaagcagat
     3001 gatgatagtg tggatccttt ctggccagcg ttacactgtt ttatggtgat tctggatcgc
     3061 cttggatcta aggtctgggg tcaacttatg gatcctattg tggcatttca aaccattatc
     3121 aacaacgcaa gctacaatag agagatccga catatacgga acagctctgt aaggaccaag
     3181 ttagaaccgg agtcctattt ggatgatatg gtgacttgca gccagatcgt atacaattac
     3241 aatcctgaaa agaccaaaaa ggattctgga tggagaacag ccatttgccc agattattgt
     3301 cctaacatgt atgaagaaat ggaaacatta gccagtgtac ttcagtcaga tattggtcaa
     3361 gacatgcgtg ttcataacag cacatttcta tggttcatcc cttttgtcca gtccctcatg
     3421 gatcttaagg atttgggtgt ggcttacata gcacaggttg ttaatcatct gtactctgaa
     3481 gtcaaagaag tcctcaacca aacagatgct gtgtgtgaca aagtcactga attttttctt
     3541 ctaattttgg tatcagtgat tgaactgcat agaaataaaa aatgtttgca tttgctgtgg
     3601 gtaagttccc agcaatgggt ggaagccgtc gtcaaatgtg ccaagcttcc taccactgcg
     3661 tttacacgga gttctgagaa atcatctgga aattgctcca aaggaacagc aatgatatct
     3721 tcactgtcat tgcattccat gccatctaac tctgtacaac ttgcttatgt gcagctgatt
     3781 agaagtctcc ttaaagaagg ttatcagctt gggcagcagt ctctttgcaa gcgattctgg
     3841 gataagctca acttattcct tagaggaaat ttatctctag gttggcagtt gactagtcag
     3901 gaaacccatg agctacaaag ttgcttaaag caaattatta gaaacataaa attcaaagca
     3961 cctccatgta acacttttgt ggatctgact tctgcatgta aaatctctcc tgcatcttat
     4021 aataaagaag aaagtgaaca aatggggaag acgtctagaa aagatatgca ttgtttggaa
     4081 gcttccagcc caacattttc taaagaacca atgaaagtgc aagacagtgt attgatcaaa
     4141 gcagataaca ctatagaagg tgacaataat gagcaaaatt atataaagga tgtgaaacta
     4201 gaggaccatc tcttagctgg gtcatgctta aagcagagta gtaaaaacat ttttactgaa
     4261 agagctgaag atcaaattaa aataagtaca aggaagcaga agtctgtaaa agagatctct
     4321 tcatatacac caaaggactg tacttcaaga aatggtccag aaaggggatg tgacagagga
     4381 ataatagtat caacacgttt gttgactgat tctagcactg atgctttgga aaaagtgtcc
     4441 acatcgaatg aagatttctc tttaaaggat gatgctcttg ctaaaacctc aaaacgaaaa
     4501 actaaggtac agaaagatga aatctgtgca aagttatcac atgtaataaa gaagcaacac
     4561 aggaagagta ctttggtcga taatactatc aatttagatg aaaatttgac tgtatctaac
     4621 attgagagtt tctattcaag gaaagataca ggagttcaga aaggagatgg tttcatacac
     4681 aatctttctt tagaccctag tggtgttctg gatgataaga atggagaaca aaaatctcaa
     4741 aacaatgtat tgccaaaaga gaaacaatta aagaatgaag aattagttat tttctctttc
     4801 catgaaaaca attgtaaaat acaggaattt catgttgatg gtaaagaatt gatccctttt
     4861 acagaaatga ccaatgcttc agagaagaaa tcatctccct ttaaagatct tatgactgta
     4921 cctgaatcaa gagatgagga gatgagtaat agtaccagtg tgatttattc taacttgaca
     4981 agagaacagg cccctgacat cagtcctaaa tctgacacct taacggattc tcagatagac
     5041 agagaccttc acaaattatc tttactagct caagccagtg ttattacgtt cccatccgat
     5101 tcacctcaga actcatcgca gctgcaaagg aaagtaaaag aagataaaag atgtttcaca
     5161 gctaaccaaa ataatgttgg agatacctcc cgtggacagg ttattattat ttcagattct
     5221 gatgatgatg atgatgaaag aatcctgagt cttgagaaac tcactaaaca ggacaaaata
     5281 tgccttgaga gggaacatcc agagcagcac gtttcaacag ttaatagtaa ggaggaaaag
     5341 aatccagtaa aggaagaaaa gacagagact ctttttcagt ttgaggaatc tgattctcag
     5401 tgttttgagt ttgaaagttc atctgaagtg ttttcagttt ggcaagatca tccagacgat
     5461 aataattcag ttcaagatgg tgagaaaaaa tgtttggctc ctatagccaa tactacaaat
     5521 ggtcagggtt gtacagatta tgtatctgaa gttgttaaaa aaggagcaga gggcattgaa
     5581 gaacacacaa gaccacggag tatttctgtt gaagaatttt gtgaaattga agtaaaaaag
     5641 cctaagagaa aacgatctga aaaaccaatg gctgaagatc ctgtgaggcc ttcatcttct
     5701 gtcagaaatg agggccagtc tgatactaat aagagagagc ttgtgggaaa tgattttaaa
     5761 agtattgata gaaggacttc aactcccaat tcacgtattc agagagccac tacggtttca
     5821 caaaagaagt cttcaaagct ttgtacttgt acagaaccca tcaggaaagt tccagtttct
     5881 aagaccccta agaaaactca ttcagatgcc aaaaaaagac agaatagaag ttcaaattac
     5941 ctaagttgta gaacaactcc tgctatagtg ccgccaaaga aatttcgtca gtgtcctgag
     6001 ccaacttcaa cagctgagaa acttggcctg aaaaagggtc ctcgtaaggc atatgagttg
     6061 tcccagcggt ctttggatta tgtagctcaa ttacgtgatc atggcaaaac tgttggagta
     6121 gttgataccc gaaaaaagac taaattaatt tctcctcaga acctgtctgt cagaaataat
     6181 aagaaacttc tgactagtca agaacttcag atgcaaaggc agatcagacc caaatcacaa
     6241 aaaaatagac gaagactttc tgattgtgaa agtacagatg ttaaaagagc agggtcacat
     6301 acagcacaga attctgacgt atttgtacca gaatctgata ggtcagatta taattgtaca
     6361 ggaggaactg aggtacttgc caacagtaac agaaaacagt taataaaatg catgccttct
     6421 gaaccagaaa ccataaaagc aaaacatggg tctccagcaa ctgatgatgc ttgccctttg
     6481 aaccagtgtg attctgtagt gttaaatgga acagtaccaa caaatgaagt aattgtctcc
     6541 acttcagaag accctctggg tggaggtgat ccaacagcac gtcatataga gatggcagct
     6601 ttgaaagaag gagagcctga ctccagcagt gatgcagagg aagataactt atttttaacc
     6661 caaaatgatc ctgaagatat ggatttatgt tcacaaatgg agaatgacaa ttataaactc
     6721 attgaactaa ttcatggaaa agatacagtt gaggttgaag aagattctgt aagtcggcct
     6781 cagttggaat ctttgagtgg cacaaagtgt aagtacaaag attgtcttga aaccacaaaa
     6841 aaccagggtg aatactgccc aaaacactct gaagtgaaag cagcagatga agatgtattt
     6901 cgtaaacctg gcttgcctcc tcctgcatct aaacctttga gacctaccac taagattttt
     6961 agctcaaaga gtacttcacg aattgctggt ctttctaaat ctttggaaac ttcttcagca
     7021 ctttcaccgt ctctaaaaaa taagtcaaag gggatacagt cgattttgaa agtaccacag
     7081 ccagttcccc tcatagctca gaagccagtt ggtgaaatga agaattcgtg caatgttctt
     7141 catcctcagt ctccgaataa ttccaacagg caaggttgca aagttccatt tggtgaaagc
     7201 aaatattttc catcttcctc tccagtaaac attcttttgt catcacagtc tgtctctgac
     7261 accttcgtta aagaggtctt aaaatggaaa tatgaaatgt ttttgaactt tggtcagtgt
     7321 gggccccctg caagtctttg tcagtccatc tcaagacctg tgcctgtcag atttcacaat
     7381 tatggagatt attttaatgt ttttttccct ttgatggtat tgaatacttt tgaaacagtg
     7441 gcacaagaat ggctcaactc tccaaataga gagaatttct atcagttgca agtacgaaaa
     7501 tttcctgccg attatataaa atactgggag tttgcagttt atctggaaga atgtgaactg
     7561 gctaaacagc tttatccaaa ggaaaacgat ttggtgtttt tagctcctga gagaataaat
     7621 gaagagaaga aagatacaga gagaaatgac atacaagatc tccacgaata tcattctggt
     7681 tatgttcata aatttcgccg cacgtcagtc atgcgtaatg ggaaaactga gtgttacctt
     7741 tccatccaga ctcaagagaa ctttccggcc aatttaaacg aacttgtgaa ttgtattgta
     7801 atcagttctc tggtaactac acaaaggaag ttgaaagcca tgtctctgtt gggtagtcgg
     7861 aaccaactgg ctagagctgt tctgaatcca aaccctatgg acttctgtac aaaagattta
     7921 ctgactacaa catctgagag aattattgcg tacttaagag atttcaatga agatcaaaag
     7981 aaagcaatag aaactgcata tgctatggtg aaacactcac catcagttgc caaaatctgc
     8041 ttgattcatg gaccacctgg aacaggaaaa tcaaaaacta ttgttggcct cctctatcgt
     8101 ctactgacag agaaccagag gaaggggcat tcagacgaaa actccaatgc caaaatcaaa
     8161 caaaaccgtg tcctcgtgtg tgcaccttcc aatgcagctg ttgatgaact catgaaaaaa
     8221 attatccttg aattcaaaga aaaatgtaaa gacaagaaga atcctttagg aaactgtgga
     8281 gatataaatt tagtacgact gggtccagaa aagtctatta atagtgaggt tctaaagttc
     8341 agtttggaca gccaagtaaa ccacagaatg aaaaaagagt taccttctca tgttcaggcg
     8401 atgcataaaa gaaaggaatt tctagattat cagctggatg agctttcccg gcagcgagct
     8461 ctatgccgag gtggacggga aatacagagg caagaattag atgaaaacat ttccaaagtt
     8521 tctaaggaaa ggcaggaact tgcttctaaa attaaagagg ttcaaggacg cccacagaaa
     8581 acacagagta tcatcatctt agagtcccat atcatctgct gcacgttgag cacaagtggt
     8641 ggtttactac ttgagtctgc tttccgtggg caagggggtg tccccttcag ctgtgtcatt
     8701 gttgatgagg ctggacagtc ttgtgaaatt gagactctta ctccactcat ccatcgctgc
     8761 aataagctca tcctagtagg agatcctaag cagctccctc cgacagtcat ctctatgaaa
     8821 gcacaggagt atggctacga ccagtcaatg atggctcgct tctgcagact gctggaagag
     8881 aatgtagaac acaacatgat cagcaggctg cccattctac agctcactgt tcagtacagg
     8941 atgcatccag acatatgcct cttcccttct aattatgttt ataacagaaa cttaaaaaca
     9001 aatagacaga cagaagccat tcgatgttca tcagattggc catttcagcc ataccttgtg
     9061 tttgatgttg gagatggttc agaaagacgg gataatgact catatataaa tgttcaagaa
     9121 ataaaactgg tgatggaaat aattaagctt attaaagaca aaagaaagga tgttagtttt
     9181 cgaaacattg gcataataac tcattacaag gcccagaaga cgatgattca gaaggatttg
     9241 gacaaagagt tcgatagaaa aggaccagca gaagtagaca ctgtggatgc attccagggt
     9301 cggcagaagg attgtgttat tgttacgtgt gtcagagcaa atagcatcca aggttcaatt
     9361 ggattcctgg caagtttgca gagattgaat gtcaccatca cacgagccaa gtacagcctc
     9421 ttcatcctcg gacatttgag gaccctgatg gaaaaccagc attggaatca gctgattcag
     9481 gatgctcaga agcgtggtgc cattattaag acctgtgaca aaaactatag acatgatgca
     9541 gtgaagattc tgaaactcaa gcctgtgctg cagagaagtc tcactcaccc tcctaccata
     9601 gccccagagg ggtccagacc ccagggtggt ttgcccagca gcaagctaga cagtggattt
     9661 gccaagacat ctgttgctgc ttctctatac cacacaccct ctgactccaa ggaaattact
     9721 cttactgtta cttcaaagga ccctgaaaga cctcctgttc atgaccaact tcaggaccca
     9781 cgactgctga agaggatggg cattgaggtc aaaggaggaa tattcctttg ggatccacaa
     9841 ccctcgagcc cccagcatcc tggagcaaca cctcctacgg gcgagccggg cttccctgtc
     9901 gttcaccagg acctgagcca tatacagcag cccgctgctg tagtggctgc tctgagcagc
     9961 cacaaacctc ccgtgcgggg cgaacctcca gctgccagtc ccgaggcttc cacgtgtcag
    10021 agcaaatgtg atgacccgga agaggagctc tgtcacagga gagaggccag ggctttcagt
    10081 gaaggggagc aggagaagtg tggttccgag acccatcaca ccaggaggaa ctctaggtgg
    10141 gacaagagga cactggagca ggaggacagc agttccaaga aaagaaagct tttataagtc
    10201 tgactacaag gacgacgatg acaagtgata accggtcatc atcaccatca ccattgagtt
    10261 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
    10321 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
    10381 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
    10441 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
    10501 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
    10561 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
    10621 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
    10681 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    10741 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    10801 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    10861 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    10921 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    10981 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    11041 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    11101 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    11161 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    11221 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
    11281 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
    11341 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
    11401 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
    11461 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
    11521 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
    11581 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
    11641 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
    11701 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
    11761 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
    11821 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
    11881 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
    11941 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
    12001 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
    12061 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
    12121 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
    12181 tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca
    12241 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    12301 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    12361 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    12421 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    12481 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
    12541 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    12601 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    12661 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    12721 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    12781 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    12841 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    12901 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    12961 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    13021 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    13081 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    13141 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    13201 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    13261 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    13321 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    13381 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    13441 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    13501 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    13561 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    13621 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    13681 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    13741 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    13801 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    13861 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    13921 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    13981 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    14041 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    14101 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    14161 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    14221 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    14281 cgaccgagtt gctcttgccc ggc
//
