LOCUS       sequence-43593-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE3k-RFP
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      151..222
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      238..265
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(503..522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
       61 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaagg
      121 atccaaactc gagtaaggat ctccaggcat caaataaaac gaaaggctca gtcgaaagac
      181 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      241 tcaccttcgg gtgggccttt ctgcgtttat acctagggcg ttcggctgcg gcgagcggta
      301 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      361 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      421 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      541 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      601 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      661 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      721 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      781 ggtaacagga ttagcagagc gaggta
//
