LOCUS       sequence-435966-       10194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter for activated NM2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Marshall-Burghardt S, Migueles-Ramirez RA, Lin Q, El Baba N, Saada 
            R, Umar M, Mavalwala K, Hayer A
  TITLE     Excitable Rho dynamics control cell shape and motility by 
            sequentially activating ERM proteins and actomyosin contractility.
  JOURNAL   Sci Adv. 2024 Sep 6;10(36):eadn6858. doi: 10.1126/sciadv.adn6858. 
            Epub 2024 Sep 6.
  PUBMED    39241071
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-09-6"; volume: "10(36):eadn6858. doi"; pages: " 
            10.1126/sciadv.adn6858. Epub 2024 Sep 6"
COMMENT     Sequence Label: pLV-mRuby3-MLC-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..10194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3394..4104
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    4746..5295
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4890..4907)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5117..5136
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5298..6092
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5352..5371)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5962..5981
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6116..6704
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6169..6189)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6587..6598)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6912..7545
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7674..7690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7674..7690)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7687..7709)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7698..7714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7722..7728,7729..7746,7747..7752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7767..7788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7905..7922)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8076..8661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8156..8175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8832..9623,9624..9692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9455..9474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9693..9797)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9845..9979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9882..9901)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9936..9955
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccaccg gtcatggtgt ctaagggcga agagctgatc
     3421 aaggaaaata tgcgtatgaa ggtggtcatg gaaggttcgg tcaacggcca ccaattcaaa
     3481 tgcacaggtg aaggagaagg cagaccgtac gagggagtgc aaaccatgag gatcaaagtc
     3541 atcgagggag gacccctgcc atttgccttt gacattcttg ccacgtcgtt catgtatggc
     3601 agccgtacct ttatcaagta cccggccgac atccctgatt tctttaaaca gtcctttcct
     3661 gagggtttta cttgggaaag agttacgaga tacgaagatg gtggagtcgt caccgtcacg
     3721 caggacacca gccttgagga tggcgagctc gtctacaacg tcaaggtcag aggggtaaac
     3781 tttccctcca atggtcccgt gatgcagaag aagaccaagg gttgggagcc taatacagag
     3841 atgatgtatc cagcagatgg tggtctgaga ggatacactg acatcgcact gaaagttgat
     3901 ggtggtggcc atctgcactg caacttcgtg acaacttaca ggtcaaaaaa gaccgtcggg
     3961 aacatcaaga tgcccggtgt ccatgccgtt gatcaccgcc tggaaaggat cgaggagagt
     4021 gacaatgaaa cctacgtagt gcaaagagaa gtggcagttg ccaaatacag caaccttggt
     4081 ggtggcatgg acgagctgta caagggagcc ggtatgtcca gcaagcgggc caaagccaag
     4141 accaccaaga agcggccaca gcgggccaca tccaatgtct tcgcaatgtt tgaccagtcc
     4201 cagatccagg agtttaagga ggctttcaac atgattgacc agaaccgtga tggcttcatt
     4261 gacaaggagg acctgcacga catgctggcc tcgctgggga agaaccccac agacgaatac
     4321 ctggagggca tgatgagcga ggccccgggg cccatcaact tcaccatgtt cctcaccatg
     4381 tttggggaga agctgaacgg cacggacccc gaggatgtga ttcgcaacgc ctttgcctgc
     4441 ttcgacgagg aagcctcagg tttcatccat gaggaccacc tccgggagct gctcaccacc
     4501 atgggtgacc gcttcacaga tgaggaagtg gacgagatgt accgggaggc acccattgat
     4561 aagaaaggca acttcaacta cgtggagttc acccgcatcc tcaaacatgg cgccaaggat
     4621 aaagacgact aggaattcct cgagggcggc cgctctagag tcgacgggcc gcggtaacaa
     4681 ttgttaacta acttaagcta gcaacggttt ccctctagcg ggatcaattc cgcccccccc
     4741 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     4801 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     4861 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     4921 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     4981 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     5041 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     5101 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     5161 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     5221 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     5281 aaaaacacga taataccatg attgaacaag atggattgca cgcaggttct ccggccgctt
     5341 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5401 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5461 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     5521 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     5581 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     5641 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     5701 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     5761 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     5821 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     5881 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     5941 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6001 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6061 ccttctatcg ccttcttgac gagttcttct gacccgggcg gggcgcgtct ggaacaatca
     6121 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6181 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6241 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6301 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6361 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6421 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6481 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     6541 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6601 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6661 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
     6721 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     6781 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gtttttccag tcacacctca
     6841 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6901 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     6961 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     7021 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     7081 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     7141 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     7201 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     7261 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     7321 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     7381 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7441 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7501 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7561 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     7621 gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7681 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7741 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7801 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7861 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7921 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7981 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8041 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8101 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8161 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8221 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8281 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8341 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8401 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8461 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8521 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8581 tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg
     8641 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8701 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8761 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8821 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8881 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8941 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9001 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9061 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9121 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9181 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9241 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9301 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9361 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9421 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9481 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9541 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9601 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9661 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9721 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9781 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     9841 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9901 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9961 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    10021 caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg
    10081 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10141 tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
