LOCUS       sequence-436034-       14866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of dCasRx-RBM25. EF1a promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14866)
  AUTHORS   Li JD, Taipale M, Blencowe BJ
  TITLE     Efficient, specific, and combinatorial control of endogenous exon 
            splicing with dCasRx-RBM25.
  JOURNAL   Mol Cell. 2024 Jul 11;84(13):2573-2589.e5. doi: 
            10.1016/j.molcel.2024.05.028. Epub 2024 Jun 24.
  PUBMED    38917795
REFERENCE   2  (bases 1 to 14866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05.028"; journalName: "Mol Cell"; date: 
            "2024-07-11- 11"; volume: "84"; issue: "13"; pages: "2573-2589.e5"
COMMENT     Sequence Label: EF1a-dCasRx-RBM25
FEATURES             Location/Qualifiers
     source          1..14866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2682..3860
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2913..3851
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3808..3828
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3879..3899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3909..6806
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             6816..6842
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             6852..6878
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    6942..6966
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9499..9523)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9558..10146
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9611..9631)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10029..10040)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10149..10165)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10150..10166)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10671..10851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10877..10894)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10883..11107
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11153..11581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11240..11259)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11450..11471
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11590..11610)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11595..11924
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11775..11910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11837..11856
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11972..12019
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12038..12412
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12542..12663
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12579..12598)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12633..12652
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12712..12728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12712..12728)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12725..12747)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12736..12752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12760..12766,12767..12784,12785..12790))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12805..12826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12943..12960)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13114..13702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13194..13213)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13873..14664,14665..14733))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14496..14515
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14734..14838)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     3001 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     3061 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     3121 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     3181 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     3241 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     3301 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     3361 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     3421 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3481 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3541 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3601 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3661 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3721 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3781 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3841 ttccatttca ggtgtcgtga cgtacggcca ccatgagccc caagaagaag agaaaggtgg
     3901 aggccagcat cgaaaaaaaa aagtccttcg ccaagggcat gggcgtgaag tccacactcg
     3961 tgtccggctc caaagtgtac atgacaacct tcgccgaagg cagcgacgcc aggctggaaa
     4021 agatcgtgga gggcgacagc atcaggagcg tgaatgaggg cgaggccttc agcgctgaaa
     4081 tggccgataa aaacgccggc tataagatcg gcaacgccaa attcagccat cctaagggct
     4141 acgccgtggt ggctaacaac cctctgtata caggacccgt ccagcaggat atgctcggcc
     4201 tgaaggaaac tctggaaaag aggtacttcg gcgagagcgc tgatggcaat gacaatattt
     4261 gtatccaggt gatccataac atcctggaca ttgaaaaaat cctcgccgaa tacattacca
     4321 acgccgccta cgccgtcaac aatatctccg gcctggataa ggacattatt ggattcggca
     4381 agttctccac agtgtatacc tacgacgaat tcaaagaccc cgagcaccat agggccgctt
     4441 tcaacaataa cgataagctc atcaacgcca tcaaggccca gtatgacgag ttcgacaact
     4501 tcctcgataa ccccagactc ggctatttcg gccaggcctt tttcagcaag gagggcagaa
     4561 attacatcat caattacggc aacgaatgct atgacattct ggccctcctg agcggactgg
     4621 cgcactgggt ggtcgctaac aacgaagaag agtccaggat ctccaggacc tggctctaca
     4681 acctcgataa gaacctcgac aacgaataca tctccaccct caactacctc tacgacagga
     4741 tcaccaatga gctgaccaac tccttctcca agaactccgc cgccaacgtg aactatattg
     4801 ccgaaactct gggaatcaac cctgccgaat tcgccgaaca atatttcaga ttcagcatta
     4861 tgaaagagca gaaaaacctc ggattcaata tcaccaagct cagggaagtg atgctggaca
     4921 ggaaggatat gtccgagatc aggaaaaatc ataaggtgtt cgactccatc aggaccaagg
     4981 tctacaccat gatggacttt gtgatttata ggtattacat cgaagaggat gccaaggtgg
     5041 ctgccgccaa taagtccctc cccgataatg agaagtccct gagcgagaag gatatctttg
     5101 tgattaacct gaggggctcc ttcaacgacg accagaagga tgccctctac tacgatgaag
     5161 ctaatagaat ttggagaaag ctcgaaaata tcatgcacaa catcaaggaa tttaggggaa
     5221 acaagacaag agagtataag aagaaggacg cccctagact gcccagaatc ctgcccgctg
     5281 gccgtgatgt ttccgccttc agcaaactca tgtatgccct gaccatgttc ctggatggca
     5341 aggagatcaa cgacctcctg accaccctga ttaataaatt cgataacatc cagagcttcc
     5401 tgaaggtgat gcctctcatc ggagtcaacg ctaagttcgt ggaggaatac gcctttttca
     5461 aagactccgc caagatcgcc gatgagctga ggctgatcaa gtccttcgct agaatgggag
     5521 aacctattgc cgatgccagg agggccatgt atatcgacgc catccgtatt ttaggaacca
     5581 acctgtccta tgatgagctc aaggccctcg ccgacacctt ttccctggac gagaacggaa
     5641 acaagctcaa gaaaggcaag cacggcatga gaaatttcat tattaataac gtgatcagca
     5701 ataaaaggtt ccactacctg atcagatacg gtgatcctgc ccacctccat gagatcgcca
     5761 aaaacgaggc cgtggtgaag ttcgtgctcg gcaggatcgc tgacatccag aaaaaacagg
     5821 gccagaacgg caagaaccag atcgacaggt actacgaaac ttgtatcgga aaggataagg
     5881 gcaagagcgt gagcgaaaag gtggacgctc tcacaaagat catcaccgga atgaactacg
     5941 accaattcga caagaaaagg agcgtcattg aggacaccgg cagggaaaac gccgagaggg
     6001 agaagtttaa aaagatcatc agcctgtacc tcaccgtgat ctaccacatc ctcaagaata
     6061 ttgtcaatat caacgccagg tacgtcatcg gattccattg cgtcgagcgt gatgctcaac
     6121 tgtacaagga gaaaggctac gacatcaatc tcaagaaact ggaagagaag ggattcagct
     6181 ccgtcaccaa gctctgcgct ggcattgatg aaactgcccc cgataagaga aaggacgtgg
     6241 aaaaggagat ggctgaaaga gccaaggaga gcattgacag cctcgagagc gccaacccca
     6301 agctgtatgc caattacatc aaatacagcg acgagaagaa agccgaggag ttcaccaggc
     6361 agattaacag ggagaaggcc aaaaccgccc tgaacgccta cctgaggaac accaagtgga
     6421 atgtgatcat cagggaggac ctcctgagaa ttgacaacaa gacatgtacc ctgttcgcaa
     6481 acaaggccgt cgccctggaa gtggccaggt atgtccacgc ctatatcaac gacattgccg
     6541 aggtcaattc ctacttccaa ctgtaccatt acatcatgca gagaattatc atgaatgaga
     6601 ggtacgagaa aagcagcgga aaggtgtccg agtacttcga cgctgtgaat gacgagaaga
     6661 agtacaacga taggctcctg aaactgctgt gtgtgccttt cggctactgt atccccaggt
     6721 ttaagaacct gagcatcgag gccctgttcg ataggaacga ggccgccaag ttcgacaagg
     6781 agaaaaagaa ggtgtccggc aattccggat ccggacctgc tgctaagaga gtgaaactgg
     6841 atgcggccgc ttacccatac gatgttccag attacgctgg cggccgcggg ggaggcggtt
     6901 ccggtggcgg cggaagcgga ggtggaggat cagggccggc cacaagtttg tacaaaaaag
     6961 ttggcaccat gtcttttcca cctcatttga atcgccctcc catgggaatc ccagcactcc
     7021 caccagggat cccacccccg cagtttccag gatttcctcc acctgtacct ccagggaccc
     7081 caatgattcc tgtaccaatg agcattatgg ctcctgctcc aactgtctta gtacccactg
     7141 tgtctatggt tggaaagcat ttgggcgcaa gaaaggatca tccaggctta aaggctaaag
     7201 aaaatgatga aaattgtggt cctactacca ctgtttttgt tggcaacatt tccgagaaag
     7261 cttcagacat gcttataaga caactcttag ctaaatgtgg tttggttttg agctggaaga
     7321 gagtacaagg tgcttccgga aagcttcaag ccttcggatt ctgtgagtac aaggagccag
     7381 aatctaccct ccgtgcactc agattattac atgacctgca aattggagag aaaaagctac
     7441 tcgttaaagt tgatgcaaag acaaaggcac agctggatga atggaaagca aagaagaaag
     7501 cttctaatgg gaatgcaagg ccagaaactg tcactaatga cgatgaagaa gccttggatg
     7561 aagaaacaaa gaggagagat cagatgatta aaggggctat tgaagtttta attcgtgaat
     7621 actccagtga gctaaatgcc ccctcacagg aatctgattc tcaccccagg aagaagaaga
     7681 aggaaaagaa ggaggacatt ttccgcagat ttccagtggc cccactgatc ccttatccac
     7741 tcatcactaa ggaggatata aatgctatag aaatggaaga agacaaaaga gacctgatat
     7801 ctcgagagat cagcaaattc agagacacac ataagaaact ggaagaagag aaaggcaaaa
     7861 aggaaaaaga aagacaggaa attgagaaag aacggagaga aagagagagg gagcgtgaaa
     7921 gggaacgaga aaggcgagaa cgggaacgag aaagggaaag agaacgtgaa cgagaaaagg
     7981 agaaagaacg ggagcgggaa cgagaacggg atagggaccg tgaccggaca aaagagagag
     8041 accgagatcg ggatcgagag agagatcgtg accgggatag agaaaggagc tcagatcgta
     8101 ataaggatcg cagtcgatca agagaaaaaa gcagagatcg tgaaagggaa cgagagcggg
     8161 aaagagagag agagagagaa cgagagcgag aacgagaacg ggagcgagag agagagcgag
     8221 agagggaacg ggagcgagaa agagaaaaag acaaaaaacg ggaccgagaa gaagatgaag
     8281 aagatgcata cgaacgaaga aaacttgaaa gaaaactccg agagaaagaa gctgcttatc
     8341 aagagcgcct taagaattgg gaaatcagag aacgaaagaa aacccgggaa tatgagaaag
     8401 aagctgaaag agaagaagaa agaagaagag aaatggccaa agaagctaaa cgactaaaag
     8461 aattcttaga agactatgat gatgatagag atgaccccaa atattacaga ggaagtgctc
     8521 ttcagaaaag gttgcgtgat agagaaaagg aaatggaagc agatgaacga gataggaaga
     8581 gagagaagga ggagcttgag gaaatcaggc agcgccttct ggcagaaggg catccagatc
     8641 cagatgcaga gctccagagg atggaacaag aggctgagag gcgcaggcag ccacaaataa
     8701 agcaagagcc agaatcagaa gaggaggaag aagaaaagca agaaaaagaa gaaaaacgag
     8761 aagaacccat ggaagaggaa gaggagccag agcaaaagcc ttgtctgaaa cctactctga
     8821 ggcccatcag ctctgctcca tctgtttcct ctgccagtgg caatgcaaca cctaacactc
     8881 ctggggatga gtctccctgt ggtattatta ttcctcatga aaactcacca gatcaacagc
     8941 aacctgagga gcataggcca aaaataggac taagtcttaa actgggtgct tccaatagtc
     9001 ctggtcagcc taattctgtg aagagaaaga aactacctgt agatagtgtc tttaacaaat
     9061 ttgaggatga agacagtgat gacgtacccc gaaaaaggaa actggttccc ttggattatg
     9121 gtgaagatga taaaaatgca accaaaggca ctgtaaacac tgaagaaaag cgtaaacaca
     9181 ttaagagtct cattgagaaa atccctacag ccaaacctga gctcttcgct tatcccctgg
     9241 attggtctat tgtggattct atactgatgg aacgtcgaat tagaccatgg attaataaga
     9301 aaatcataga atatataggt gaagaagaag ctacattagt tgattttgtt tgttctaagg
     9361 ttatggctca tagttcaccc cagagcattt tagatgatgt tgccatggta cttgatgaag
     9421 aagcagaagt ttttatagtc aaaatgtgga gattattgat atatgaaaca gaagccaaga
     9481 aaattggtct tgtgaagttg ccaactttct tgtacaaagt ggttgctcta taggaattcg
     9541 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     9601 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     9661 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     9721 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     9781 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     9841 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     9901 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     9961 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
    10021 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
    10081 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
    10141 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
    10201 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
    10261 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
    10321 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    10381 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
    10441 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
    10501 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
    10561 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
    10621 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
    10681 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    10741 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    10801 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
    10861 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
    10921 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
    10981 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
    11041 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
    11101 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
    11161 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
    11221 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
    11281 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    11341 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    11401 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    11461 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    11521 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    11581 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    11641 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    11701 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    11761 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    11821 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
    11881 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
    11941 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
    12001 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
    12061 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
    12121 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
    12181 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
    12241 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
    12301 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
    12361 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
    12421 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    12481 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    12541 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    12601 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    12661 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
    12721 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    12781 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    12841 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    12901 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    12961 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    13021 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    13081 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    13141 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    13201 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    13261 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    13321 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    13381 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    13441 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    13501 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    13561 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    13621 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    13681 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    13741 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    13801 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    13861 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    13921 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    13981 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    14041 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    14101 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    14161 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    14221 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    14281 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    14341 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    14401 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    14461 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    14521 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    14581 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    14641 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    14701 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    14761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    14821 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac
//
