LOCUS       sequence-43609-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE7c-RFP
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      130..201
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      217..244
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(482..501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
       61 cgaacgtgct gaaggtcgtc actccaccgg tgcttaagga tccaaactcg agtaaggatc
      121 tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      181 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      241 tgcgtttata cctagggcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      781 aggtatgt
//
