LOCUS       sequence-436100-        9993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an EZH2 mutant for knockout/rescue experiments in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Gail EH, Healy E, Flanigan SF, Jones N, Ng XH, Uckelmann M, Levina 
            V, Zhang Q, Davidovich C
  TITLE     Inseparable RNA binding and chromatin modification activities of a 
            nucleosome-interacting surface in EZH2.
  JOURNAL   Nat Genet. 2024 May 14. doi: 10.1038/s41588-024-01740-8.
  PUBMED    38744974
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2024 May 14. doi: 10.1038/s41588-024-01740-8."
COMMENT     Sequence Label: pHIV-EGFP.Flag-EZH2-mt2
FEATURES             Location/Qualifiers
     source          1..9993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             651..831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1340..1356
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1341..1357
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(1369..2082,2083..2085,2086..2088))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1372..2082,2083..2085,2086..2088))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1414..1435)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1763..1782
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2022..2043
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(2089..2097,2098..2100,2101..2675)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2262..2281)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2491..2508
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(2695..2715)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4966..4989)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5028..6206)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5037..5975
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5060..5080)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6269..6386
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6684..6728)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6913..7146
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7643..7768
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7815..7995
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8013..8211)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8038..8058)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8213..8592
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8764..8783
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8858..8962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8963..9031,9032..9823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9181..9200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggccgcttt acttgtacag
     1381 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     1441 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
     1501 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     1561 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
     1621 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
     1681 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     1741 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     1801 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     1861 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
     1921 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     1981 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     2041 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg ttgtggccat
     2101 attatcatcg tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg cctagacgtt
     2161 tttttaacct cgactaaaca catgtaaagc atgtgtaccg aggccccaga tcagatccca
     2221 tacaatgggg taccttctgg gcatccttca gccccttgtt gaatacgctt gaggagagcc
     2281 atttgactct ttccacaact atccaactca caacgtggca ctggggttgt gccgcctttg
     2341 caggtgtatc ttatacacgt ggcttttggc cgcagaggca cctgtcgcca ggtggggggt
     2401 tccgctgcct gcaaagggtc gctacagacg ttgtttgtct tcaagaagct tccagaggaa
     2461 ctgcttcctt cacgacattc aacagacctt gcattccttt ggcgagaggg gaaagacccc
     2521 taggaatgct cgtcaagaag acagggccag gtttccgggc cctcacattg ccaaaagacg
     2581 gcaatatggt ggaaaataac atatagacaa acgcacaccg gccttattcc aagcggcttc
     2641 ggccagtaac gttagggggg ggggagggag aggggcggat cctagcccct attatacctt
     2701 tctcttcttt tttggaggga tttccatttc tctttcgatg ccgacatact tcagggcatc
     2761 agcctggctg tatctgtaat caaaaaacag ctcttcgcca gtctggatgg ctctcttggc
     2821 aaaaatacct atcctgtgat caccgttaac catcataact tttgcatagc agtttggatt
     2881 taccgaatga tttgcaaaac gaattttgtt acccttgcgg gttgcatcca ccacaaaatc
     2941 attgttcaag ttgaacagaa agctgcacat gtatttatca tacactttcc ctcttctgtc
     3001 agcttcatct tgagaaataa tctctccaca gtattctgag atgaattcat ttttctgcac
     3061 aggatctttg ataaaaatcc cccagcctgc cacgtcagat ggtgccagca atagatgctt
     3121 tttggagccc cgctgaatac tgcagttctt gcaggacaca tttttactgt cccaatggtc
     3181 agcggctcca caagtaagac agaggtcagg gtcacactct cggacagcca ggtagcacgg
     3241 gcactgcttg gtgttgcact gtgctttgca gcggcatccc ggaaagcggt tttgacactc
     3301 tgaactacat tgacaaaact tttcacaaaa attttgtgct atcacacaag ggcacgaact
     3361 gtcacaaggc tgccgtggat gatcacaggg ttgatagttg taaacatggt tagaggagcc
     3421 gtcctttttc agctgtatct ttctgcagtg tgcagcccac aaccggtgtt tactcctaat
     3481 agcagcatta ggagtatcca catcctcagc gggagctgga gctatgatgc tagattcttt
     3541 gactctaaac tcatacacct gtctacatgt tttggtccca attaacctag caatggcaca
     3601 gaaattgtca tagtaagtgc caatgaggac tctaaacatt gaggcttcag caccactcca
     3661 ctccacattc tcaggaggtt caatatttgg cttcatcttt attggtgttt gacaccgaga
     3721 atttgcttca gaggagctcg aagtttcatc tttcttctct tcttcttctt tatcattgtt
     3781 ctctcccccc gtttcagtcc ctgcttccct atcactgtct gtatcctttg attccagcac
     3841 attaatggtg ggggtgctgg gcctgctact gttattggga agccgtcctc ttctgcggcc
     3901 tcctggacgt tttggtgggg tctttatccg ctcagcggtg agagcagcag caaactcctt
     3961 tgctccctcc aaatgctggt aacactgtgg tccacaaggt ttgttgtcta gagctgtttc
     4021 tgtgttcttc cgcttataag tgttgggtgt tgcatgaaaa gaataattgc acttacgatg
     4081 taggaagcag tcatatttaa aacatcgcct acagaaaagc gtatgaaagg agtgtaagct
     4141 ttgctctctc tgaacagatt tagcatttgg tccatctatg ttgggggtac attcaggagg
     4201 aagtgcgcct gggagctgct gttcggtgag ttctttatat ttttccttta gttcttctgc
     4261 tgtgccctta tctggaaaca ttgaggaaat ggcttcaaaa attttatcag aaggaaattt
     4321 ccgaggtggg cggctttctt tatcatctcg gtgatcctcc agatctttct gcttttcttc
     4381 tctttcttca ggatcgtctc catcatcatc atcgtcatca tcattatatt gaccaagggc
     4441 attcaccaac tccacaaaaa tttcatcatt tataaaccca cattctctat ccccgtgtac
     4501 tttcccatca taatttttta ttagttcttc aatgaaagta ccatcctgat ctaaaacttc
     4561 atctcccata taaggaatgt tatgtaaaac agtttcatct tccaccataa aattctgctg
     4621 taggggtgac caagaataca ttatgggtac tgaagcaact gcattcagag tctttaatgg
     4681 gatgacttgt gttggaaaat ccaagtcact ggtcaccgaa cactccctag tcccgcgcaa
     4741 tgagctcaca gaagtcagga tgtgcacagg ctgtatcctt cgctgtttcc attcttggtt
     4801 taagatttcc gttctttcca aaattttctg acgattggaa ctaaacatac tagctacttc
     4861 agcagcagct ctagccctct tgagctgtct cagtcgcatg tactctgatt ttacacgctt
     4921 ccgccaacaa actggtccct tctcagattt cttcccagtc tggcccttgt cgtcatcgtc
     4981 tttgtagtcc atggtgggta ctctagaata gttaactcag cggccgctca cgacacctga
     5041 aatggaagaa aaaaactttg aaccactgtc tgaggcttga gaatgaacca agatccaaac
     5101 tcaaaaaggg caaattccaa ggagaattac atcaagtgcc aagctggcct aacttcagtc
     5161 tccacccact cagtgtgggg aaactccatc gcataaaacc cctcccccca acctaaagac
     5221 gacgtactcc aaaagctcga gaactaatcg aggtgcctgg acggcgcccg gtactccgtg
     5281 gagtcacatg aagcgacggc tgaggacgga aaggcccttt tcctttgtgt gggtgactca
     5341 cccgcccgct ctcccgagcg ccgcgtcctc cattttgagc tccctgcagc agggccggga
     5401 agcggccatc tttccgctca cgcaactggt gccgaccggg ccagccttgc cgcccagggc
     5461 ggggcgatac acggcggcgc gaggccaggc accagagcag gccggccagc ttgagactac
     5521 ccccgtccga ttctcggtgg ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg
     5581 gacgcacggg ccccgtcgcc gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct
     5641 tggcccgcat ttacaagact atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa
     5701 ttttaaatgg ctagagactt atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt
     5761 cgcacgcggc ggccccagcg cccaggccag gcctcaactc aagcacgagg cgaaggggct
     5821 ccttaagcgc aaggcctcga actctcccac ccacttccaa cccgaagctc gggatcaaga
     5881 atcacgtact gcagccaggt ggaagtaatt caaggcacgc aagggccata acccgtaaag
     5941 aggccaggcc cgcgggaacc acacacggca cttacctgtg ttctggcggc aaacccgttg
     6001 cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc accctcggga
     6061 aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag
     6121 gcaccggttc aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc
     6181 tctgcccact gacgggcacc ggagcctcac gctagagcgg gcccggtact aaccaaactg
     6241 gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta
     6301 attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc
     6361 ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt ctgcagaatt
     6421 ggcgcacgca gtgccgatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca
     6481 ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga
     6541 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
     6601 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
     6661 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
     6721 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
     6781 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
     6841 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
     6901 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
     6961 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
     7021 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
     7081 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
     7141 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
     7201 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
     7261 tccctcatat ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc tgtgcggtgg
     7321 tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct
     7381 ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt
     7441 ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg
     7501 tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat
     7561 gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc
     7621 taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc
     7681 tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt
     7741 cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc
     7801 tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga gggatctcta
     7861 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
     7921 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
     7981 taaccagaga gacccagtac aggcaaaacg cgctgcttat atagacctcc caccgtacac
     8041 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     8101 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     8161 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     8221 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     8281 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     8341 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     8401 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     8461 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     8521 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
     8581 ataatcaatg tcaacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa
     8641 ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaattt
     8701 tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac tggcttaact
     8761 atgcggcatc agagcagatt gtactgagag tgcaccatag gggatcggga gatctcccga
     8821 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8881 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8941 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     9001 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     9061 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     9121 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     9181 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     9241 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     9301 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     9361 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     9421 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     9481 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     9541 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     9601 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     9661 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     9721 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     9781 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     9841 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     9901 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     9961 gtcagacccc gtagaaaaga tcaaaggatc ttc
//
