LOCUS       sequence-43611-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE7k-RFP
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      148..219
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      235..262
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(500..519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
       61 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaaggatc
      121 caaactcgag taaggatctc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      181 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      241 ccttcgggtg ggcctttctg cgtttatacc tagggcgttc ggctgcggcg agcggtatca
      301 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      361 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      421 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      481 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      541 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      601 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      661 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      721 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      781 aacaggatta gcagagcgag gtatg
//
