LOCUS       sequence-436111-        4630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for constitutive expression of TAF5 alternative exon-8 mini 
            gene with mutated SRSF1 motif (AGTCTCCT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Xiao MS, Damodaran AP, Kumari B, Dickson E, Xing K, On TA, Parab N, 
            King HE, Perez AR, Guiblet WM, Duncan G, Che A, Chari R, Andresson 
            T, Vidigal JA, Weatheritt RJ, Aregger M, Gonatopoulos-Pournatzis T
  TITLE     Genome-scale exon perturbation screens uncover exons critical for 
            cell fitness.
  JOURNAL   Mol Cell. 2024 Jul 11;84(13):2553-2572.e19. doi: 
            10.1016/j.molcel.2024.05.024. Epub 2024 Jun 24.
  PUBMED    38917794
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05.024"; journalName: "Mol Cell"; date: 
            "2024-07-11- 11"; volume: "84"; issue: "13"; pages: "2553-2572.e19"
COMMENT     Sequence Label: pET01-TAF5-ex8-Minigene-SRSF1 motif MUT
FEATURES             Location/Qualifiers
     source          1..4630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(107..126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     1285..1304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(1859..2055)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1873..2008
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2163..2182
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4328..4346)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4414..4518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4519..4587,4588..4630,1..749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
       61 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      121 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      421 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
      781 agattgattt aaaacttcat ttttaattta aaaggatcta ggctgctgct tgcaaacaaa
      841 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      901 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
      961 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1021 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1081 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1141 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1201 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1261 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1321 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1381 gaaaaacgcc agcaacggag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg
     1441 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
     1501 caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt
     1561 ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc
     1621 tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat
     1681 cagcggtcca atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc
     1741 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     1801 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg gggagctttt
     1861 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     1921 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     1981 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
     2041 actaattgag atgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     2101 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     2161 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     2221 acgatgcgtc cggcgtagag gatctcagga tatagtagtt tcgcttttgc atagggaggg
     2281 ggaaatgtag tcttatgcaa tactcttgta gtcttgcaac atgcttatgt aacgatgagt
     2341 tagcaacatg ccttataagg agagaaaaag caccgtgcat gccgattggt gggagtaagg
     2401 tggtatgatc gtggtatgat cgtgccttgt taggaaggca acagacgggt ctaacacgga
     2461 ttggacgaac cactgaattc cgcattgcag agatattgta tttaagtgcc tagctcgata
     2521 caataaacgc catttgacca ttcaccacat tggtgtgcac ctcaagcttc ctgcatgctg
     2581 ctgctgctgc tgctgctggg cctgaggcta cagctctccc tgggcatcat cccagttgag
     2641 gaggagaacc cggacttctg gaaccgcgag gcagccgagg ccctgggtgc cgccaagaag
     2701 ctgcagcctg cacagacagc cgccaagaac ctcatcatct tcctgggcga tgggatgggg
     2761 gtgtctacgg tgacagctgc caggatcgat ccgcttcctg cccctgctgg ccctgctcat
     2821 cctctgggag ccccgccctg cccaggcttt tgtcaaacag cacctttgtg gttctcactt
     2881 ggtggaagct ctctacctgg tgtgtgggga gcgtggattc ttctacacac ccatgtcccg
     2941 ccgcgaagtg gaggacccac aaggtaagct ctgctcctga attaattcta tcccaagtgc
     3001 taactaccct gtttgtcttt cacccttgag accttgtaaa ttgtgcccta ggtgtggagg
     3061 gtctcaggct aaccagtggg gggcacattt ctgtgggcag ctagacatat gtaaacatgg
     3121 tagctgccag gaaggagtga gaatccttcc ttaagtctcc taggtggtga cgggtggcta
     3181 ggccccagga taggtaccgg gccgctcggt atatgtagag tgtcagggtt aacattagtt
     3241 tggttagttc tttcaattga tacctactgt gaattgcgtg tatgaattaa aagtgagatg
     3301 cagcatgtta ggtatattta atatagtcaa atatatataa aatattaaat gtattcaaat
     3361 gtatataaaa ctgggcaggt gaccaaaggt tactctggga gtcaaatata tcaccttctc
     3421 ttctctcagg aactatctgc tttcctctag tctcctcgga actgttagat tgtggagcct
     3481 tcaaacattt acttgtttgg tgggatataa aggacacaac tatccagtat gggacacaca
     3541 attttctcca tatggatatt attttgtgtc agggggccat gaccgagtag ctcggtaaga
     3601 acactgtgat cttatgactg ggtctattca atgaacagaa tggtttcttg ttgggaatta
     3661 cacagccagc caaattcatt tacacttcca ttattgaggt tcaactttct gagaagcttt
     3721 tgggccgcca ccgcggtgga gctcggtacc tatttgggga ccccatagag cactgcactg
     3781 actgagggat ggtaacagga tgtgtaggtt ttggaggccc atatgtccat tcatgaccag
     3841 tgacttgtct cacagccatg caacccttgc ctcctgtgct gacttagcag gggataaagt
     3901 gagagaaagc ctgggctaat cagggggtcg ctcagctcct cctaactgga ttgtcctatg
     3961 tgtctttgct tctgtgctgc tgatgctctg ccctgtgctg acatgacctc cctggcagtg
     4021 gcacaactgg agctgggtgg aggcccgtga ccttcagacc ttggcactgg aggtggcccg
     4081 gcagaagcgc ggcatcgtgg atcagtgctg caccagcatc tgctctctct accaactgga
     4141 gaactactgc aactaggccc accactaccc tgtccacccc tctgcaatga ataaaacctt
     4201 tgaaagagca ctacaagttg tgtgtacatg cgtgcatgtg catatgtggt gcggggggaa
     4261 catgagtggg gctggctgga gtggcgatga taagctgtca aacatgagaa ttcttgaaga
     4321 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     4381 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4441 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4501 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4561 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4621 gaagatcagt
//
