LOCUS       sequence-43612-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE7k-RFP
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    209..225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(229..248)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(230..248)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(336..355)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        561..638
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(658..677)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gaacggcagc ggaccacctt tggtaacttt cagtttagcg gtctgggtac cttcgtacgg
       61 acgaccttca ccttcacctt cgatttcgaa ctcgtgaccg ttaacggaac cttccatacg
      121 aactttgaaa cgcatgaact ctttgataac gtcttcgcta ctcgccatat gtatatctcc
      181 ttcttaaaag atcttttgaa ttctgaaatt gttatccgct cacaattccc ctatagtgag
      241 tcgtattaat ttcgcgggat cgagatcgat ctcgatcctc tacgccggac gcatcgtggc
      301 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
      361 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
      421 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
      481 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
      541 gggagagcgt cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat
      601 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg
      661 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
      721 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
      781 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
      841 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
      901 atcaactggg tgccagcgtg gtggtgtcga
//
