LOCUS       sequence-436176-        8365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blue light inducible system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Park JH, Hollo G, Schaerli Y
  TITLE     From resonance to chaos by modulating spatiotemporal patterns 
            through a synthetic optogenetic oscillator.
  JOURNAL   Nat Commun. 2024 Aug 23;15(1):7284. doi: 10.1038/s41467-024-51626-w.
  PUBMED    39179558
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-08-23"; volume: "15(1):7284. doi"; pages: " 
            10.1038/s41467-024-51626-w"
COMMENT     Sequence Label: pJP_Azul01
FEATURES             Location/Qualifiers
     source          1..8365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      952..1023
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1039..1066
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        1147..1196
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     misc_feature    2386..2406
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     promoter        complement(4387..4421)
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     promoter        4496..4573
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     terminator      6461..6547
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(6582..6599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6716..7261)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6796..6815)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7426..8217)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAADELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccacgcc cgtgatcctg
       61 atcaccggct atccggacga aaacatctcg acccgggccg ccgaggccgg cgtaaaagac
      121 gtggttttga agccgcttct cgacgaaaac ctgctcaagc gtatccgccg cgccatccag
      181 gaccggcctc gggcatgacc tacggggttc tacgtaaggc acccccctta agatatcgct
      241 cgaaattttc gaacctcccg ataccgcgta ccaatgcgtc atcacaacgg agctatggac
      301 tatgtttaaa aaacgagaat actatatggc acgtaccccg agccgtagca gcattggtag
      361 cctgcgtagt ccgcataccc ataaagcaat tctgaccagc accattgaaa tcctgaaaga
      421 atgtggttat agcggtctga gcattgaaag cgttgcacgt cgtgccggtg caagcaaacc
      481 gaccatttat cgttggtgga ccaataaagc agcactgatt gccgaagtgt atgaaaatga
      541 aagcgaacag gtgcgtaaat ttccggatct gggtagcttt aaagccgatc tggattttct
      601 gctgcgtaat ctgtggaaag tttggcgtga aaccatttgt ggtgaagcat ttcgttgtgt
      661 tattgcagaa gcacagctgg accctgcaac cctgacccag ctgaaagatc agtttatgga
      721 acgtcgtcgt gagatgccga aaaaactggt tgaaaatgcc attagcaatg gtgaactgcc
      781 gaaagatacc aatcgtgaac tgctgctgga tatgattttt ggtttttgtt ggtatcgcct
      841 gctgaccgaa cagctgaccg ttgaacagga tattgaagaa tttaccttcc tgctgattaa
      901 tggtgtttgt ccgggtacac agcgttaact agggcccata cccccaggca tcaaataaaa
      961 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1021 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacgacgtac
     1081 ggtggaatct gattcgttac caattgacat gatacgaaac gtaccgtatc gttaaggtta
     1141 ctagagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc
     1201 tacaaataat tttgtttaaa ctatatgagc atcgcggcga ccctggagaa cgatctggcg
     1261 cgtctggaaa acgaaaacgc tcgtctcgaa aaagacatcg cgaacctgga acgtgacctg
     1321 gcgaaactgg agcgtgaaga agcgtacttc ggaggttcag gtggtaagaa cactggtgaa
     1381 atctctgaga aagtcaagct gggcactaag gcactggctg gtcaatggct ggcttacggt
     1441 gttactcgca gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc caaagagttc
     1501 ggcttccgtc aacaagtgct ggaagatacc attcagccag ctattgattc cggcaagggt
     1561 ctgatgttca ctcagccgaa tcaggctgct ggatacatgg ctaagctgat ttgggaatct
     1621 gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag
     1681 ctgctggctg ctgaggtcaa agataagaag actggagaga ttcttcgcaa gcgttgcgct
     1741 gtgcattggg taactcctga tggtttccct gtgtggcagg aatacaagaa gcctattcag
     1801 aagcgcctgg acatgatttt cttgggtcaa tttcgcttgc aacctaccat taacaccaac
     1861 aaagatagcg agattgatgc acacaaacag gagtctggta tcgctcctaa ctttgtacac
     1921 agccaagacg gtagccacct tcgtaagact gtagtgtggg cacacgagaa gtacggaatc
     1981 gaatcttttg cactgattca cgactccttc ggtacgattc cggctgacgc tgcgaacctg
     2041 ttcaaagcag tgcgcgaaac tatggttgac acatatgagt cttgtgatgt actggctgat
     2101 ttctacgacc agttcgctga ccagttgcac gagtctcaat tggacaaaat gccagcactt
     2161 ccggctaaag gtaacttgaa cctccgtgac atcttagagt cggacttcgc gttcgcgtaa
     2221 taatctagac caattattga acacccttcg gggtgttttt ttgtttctgg tctccaacgt
     2281 ctcattttcg ccagatatcg gaccaaaacg aaaaaaggcc cccctttcgg gaggcctctt
     2341 ttctggaatt tggtaccgag ctaactaact aacccttagt gactcctgca gcggccgcta
     2401 ctagtattat ttgtaagctt cgatttcttt acgcaggttc gcgattttct gcttcagttg
     2461 ttcacgtttc tgtttcagct gttcgatacg gttgcgcagt tccgcttttt tggatttcag
     2521 ttcttctttt tcgttggatc caccacctga acctccgttc tcatcggtca cggtaactac
     2581 ttcgttatcg gtcccattga ttgcgtctgc ttgtagaatc tcgttgactt tcttagcaac
     2641 aatcccgtag atgtcctgaa cggtttcact aggaagcaag ttaaccgcgc gaccacctac
     2701 ctcatctcgg agcatcgcgg agaagtgctg gatgccagag caagacccgt caaacgccag
     2761 cggaagggag cagttatagc tcaggccgtg gtgctgtacc ccagcgtact caaagcagaa
     2821 cgcaaggaag cagaacggag aatcttgctc agcccaccaa gtgttctcca gtggagactt
     2881 agcgcaagcc atgatgttct cgtggttttc ctcaatgaac ttgatgcgct cagggaacgg
     2941 aaccttgtcg acacccgcac agtttgcacc gtggattttc agccagtagt aaccttcctt
     3001 accgattggt ttacctttcg ccagcgtaag cagtcctttg gtcatatcgt taccttgcgg
     3061 gttgaacatt gacacagcgt aaacacgacc gcgccagtcc atgttgtaag ggaaccagat
     3121 ggccttatgg ttagcaaact tattggcttg ctcaagcatg aactcaaggc tgatacggcg
     3181 agacttgcga gccttgtcct tgcggtacac agcagcggca gcacgtttcc acgcggtgag
     3241 agcctcagga ttcatgtcga tgtcttccgg tttcatcggg agttcttcac gctcaatcgc
     3301 agggatgtcc tcgaccggac aatgcttcca cttggtgatt acgttggcga ccgctaggac
     3361 tttcttgttg attttccatg cggtgttttg cgcaatgtta atcgctttgt acacctcagg
     3421 catgtaaacg tcttcgtagc gcatcagtgc tttcttactg tgagtacgca ccagcgccag
     3481 aggacgacga ccgttagccc aatagccacc accagtaatg ccagtccacg gcttaggagg
     3541 aactacgcaa ggttggaaca tcggagagat gccagccagc gcacctgcac gggttgcgat
     3601 agcctcagcg tattcaggtg cgagttcgat agtctcagag tcttgaccta ctacgccagc
     3661 attttggcgg tgtaagctaa ccattccggt tgactcaatg agcatctcga tgcagcgtac
     3721 tcctacatga atagagtctt ccttatgcca cgaagaccac gcctcgccac cgagtagacc
     3781 cttagagagc atgtcagcct cgacaacttg cataaatgct ttcttgtaga cgtgccctac
     3841 gcgcttgttg agttgttcct caacgttttt cttgaagtgc ttagcttcaa ggtcacggat
     3901 acgaccgaag cgagcctcgt cctcaatggc ccgaccgatt gcgcttgcta cagcctgaac
     3961 ggttgtattg tcagcactgg ttaggcaagc cagagtggtc ttaatggtga tgtacgctac
     4021 ggcttccggc ttgatttctt gcaggaactg gaaggctgtc gggcgcttgc cgcgcttagc
     4081 tttcacttcc tcaaaccagt cgttgatgcg tgcaatcatc ttagggagta gggtagtgat
     4141 gagaggcttg gcggcagcgt tatccgcaac ctcaccagct ttaagttgac gctcaaacat
     4201 cttgcggaag cgtgcttcac ccatctcgta agactcatgc tcaagggcca actgttcgcg
     4261 agctaaacgc tcaccgtaat ggtcagccag agtgttgaac gggatagcag ccagttcgat
     4321 gtcagagaag tcgttcttag cgatgttaat cgtgttcatc tagtactttc ataaatgact
     4381 ctagtagcta gcatagtacc taggactgag ctagccgtaa acaatccaca gcaggcatac
     4441 aaccgcacac cgggttactc cgttctacag cataagggag agcgtcgaga tcccggacac
     4501 catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
     4561 gagggtggtg aatgtggcta gttttcaatc atttgggata ccaggacagc tggaagtcat
     4621 caaaaaagca cttgatcacg tgcgagtcgg tgtggtaatt acagatcccg cacttgaaga
     4681 taatcctatt gtctacgtaa atcaaggctt tgttcaaatg accggctacg agaccgagga
     4741 aattttagga aagaactgtc gcttcttaca ggggaaacac acagatccgg cagaagtgga
     4801 caacatcaga accgctttac aaaataaaga accggtcacc gttcagatcc aaaactacaa
     4861 aaaagacgga acgatgttct ggaatgaatt aaatattgat ccaatggaaa tagaggataa
     4921 aacgtatttt gtcggcattc agaatgatat caccgagcac cagcagaccc aggcgcgtct
     4981 ccaggaactg caatccgagc tcgtccacgt ctccaggctg agcgccatgg gcgaaatggc
     5041 gtccgcgctc gcgcacgagc tcaaccagcc gctggcggcg atcagcaact acatgaaggg
     5101 ctcgcggcgg ctgcttgccg gcagcagtga tccgaacaca ccgaaggtcg aaagcgccct
     5161 ggaccgcgcc gccgagcagg cgctgcgcgc cggccagatc atccggcgcc tgcgcgactt
     5221 cgttgcccgc ggcgaatcgg agaagcgggt cgagagtctc tccaagctga tcgaggaggc
     5281 cggcgcgctc gggcttgccg gcgcgcgcga gcagaacgtg cagctccgct tcagtctcga
     5341 tccgggcgcc gatctcgttc tcgccgaccg ggtgcagatc cagcaggtcc tggtcaacct
     5401 gttccgcaac gcgctggaag cgatggctca gtcgcagcga cgcgagctcg tcgtcaccaa
     5461 cacccccgcc gccgacgaca tgatcgaggt cgaagtgtcc gacaccggca gcggtttcca
     5521 ggacgacgtc attccgaacc tgtttcagac tttcttcacc accaaggaca ccggcatggg
     5581 cgtgggactg tccatcagcc gctcgatcat cgaagctcac ggcgggcgca tgtgggccga
     5641 gagcaacgca tcgggcgggg cgaccttccg cttcaccctc ccggcagccg acgagatgat
     5701 aggaggtcta gcatgacgac caagggacat atctacgtca tcgacgacga cgcggcgatg
     5761 cgggattcgc tgaatttcct gctggattct gccggcttcg gcgtcacgct gtttgacgac
     5821 gcgcaagcct ttctcgacgc cctgccgggt ctctccttcg gctgcgtcgt ctccgacgtg
     5881 cgcatgccgg gccttgacgg catcgagctg ttgaagcgga tgaaggcgca gcaaagcccc
     5941 tttccgatcc tcatcatgac cggtcacggc gacgtgccgc tcgcggtcga ggcgatgaag
     6001 ttaggggcgg tcgactttct ggaaaagcct ttcgaggacg accgcctcac cgccatgatc
     6061 gaatcggcga tccgccaggc cgagccggcc gccaagagcg aggccgtcgc gcaggatatc
     6121 gccgcccgcg tcgcctcgtt gagccccagg gagcgccagg tcatggaagg gctgatcgcc
     6181 ggcctttcca acaagctgat cgcccgcgag tacgacatca gcccgcgcac catcgaggtg
     6241 tatcgggcca acgtcatgac caagatgcag gccaacagcc tttcggagct ggttcgcctc
     6301 gcgatgcgcg ccggcatgct caacgattaa taacaattac tcggtaccaa agacgaacaa
     6361 taagacgctg aaaagcgtct tttttcgttt tggtcctaaa aaggccgcct gcagatatcc
     6421 atcacactgg cggccgctta attaattaat ctagaggcat caaataaaac gaaaggctca
     6481 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     6541 gacaaatccg ccgccctaga cctagggata tattccgctt cctcgctcac tgactcgcta
     6601 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
     6661 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
     6721 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
     6781 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
     6841 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
     6901 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     6961 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7021 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
     7081 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
     7141 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
     7201 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
     7261 agaagatcat cttattaatc agataaaata ttactagatt tcagtgcaat ttatctcttc
     7321 aaatgtagca cctgaagtca gccccatacg atataagttg ttactatttt gttatcaaat
     7381 aaaaaaggcc ccccgttagg gaggccttat tgttcgtccg agctcttatt tgccgactac
     7441 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
     7501 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
     7561 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
     7621 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
     7681 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
     7741 ttcaggaacc ggatcaaaga gttcatccgc cgctggacct accaaggcaa cgctatgttc
     7801 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
     7861 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
     7921 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
     7981 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
     8041 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
     8101 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
     8161 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatacg
     8221 aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg
     8281 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     8341 agctggcaat tccggttcgc ttgct
//
