LOCUS       sequence-436197-        9322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; 
            Thr1273Asp substitution).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Dey D, Qing E, He Y, Chen Y, Jennings B, Cohn W, Singh S, Gakhar L, 
            Schnicker NJ, Pierce BG, Whitelegge JP, Doray B, Orban J, Gallagher 
            T, Hasan SS
  TITLE     A single C-terminal residue controls SARS-CoV-2 spike trafficking 
            and incorporation into VLPs.
  JOURNAL   Nat Commun. 2023 Dec 15;14(1):8358. doi: 10.1038/s41467-023-44076-3.
  PUBMED    38102143
REFERENCE   2  (bases 1 to 9322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-12-15"; volume: "14(1):8358. doi"; pages: " 
            10.1038/s41467-023-44076-3"
COMMENT     Sequence Label: SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp
FEATURES             Location/Qualifiers
     source          1..9322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(19..607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(99..118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(778..1569,1570..1638))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1401..1420
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1639..1743)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1818..1837)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2009..2388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2389..2592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2543..2563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2637..2656
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2637..2655
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6600..6629
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6645..6662
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6685..6702)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6691..6915
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6961..7389
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7048..7067)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7258..7279
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7398..7418)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7403..7732
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7583..7718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7645..7664
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7799..8593
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7853..7872)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8463..8482
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8769..8890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8806..8825)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8860..8879
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8939..8955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8939..8955)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8952..8974)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8963..8979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8987..8993,8994..9011,9012..9017))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9032..9053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9170..9187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
       61 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      121 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      181 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      241 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      301 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      361 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      421 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      481 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      541 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      601 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      661 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      721 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1681 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1741 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     1801 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     1861 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     1921 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     1981 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     2041 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     2101 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2161 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2221 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2281 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2341 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2401 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2461 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2521 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2581 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
     2641 acgactcact atagggagac ccaagctggc tagttaagct tggtaccgag ctcggatcca
     2701 ctagtccagt gtggtggaat tcgagctcgg tacctcgcga atgcatctag atgccaccat
     2761 gttcgtcttc ctggtcctgc tgcctctggt ctcctcacag tgcgtcaatc tgacaactcg
     2821 gactcagctg ccacctgctt atactaatag cttcaccaga ggcgtgtact atcctgacaa
     2881 ggtgtttaga agctccgtgc tgcactctac acaggatctg tttctgccat tctttagcaa
     2941 cgtgacctgg ttccacgcca tccacgtgag cggcaccaat ggcacaaagc ggttcgacaa
     3001 tcccgtgctg ccttttaacg atggcgtgta cttcgcctct accgagaaga gcaacatcat
     3061 cagaggctgg atctttggca ccacactgga ctccaagaca cagtctctgc tgatcgtgaa
     3121 caatgccacc aacgtggtca tcaaggtgtg cgagttccag ttttgtaatg atcccttcct
     3181 gggcgtgtac tatcacaaga acaataagag ctggatggag tccgagttta gagtgtattc
     3241 tagcgccaac aactgcacat ttgagtacgt gagccagcct ttcctgatgg acctggaggg
     3301 caagcagggc aatttcaaga acctgaggga gttcgtgttt aagaatatcg acggctactt
     3361 caaaatctac tctaagcaca cccccatcaa cctggtgcgc gacctgcctc agggcttcag
     3421 cgccctggag cccctggtgg atctgcctat cggcatcaac atcacccggt ttcagacact
     3481 gctggccctg cacagaagct acctgacacc cggcgactcc tctagcggat ggaccgccgg
     3541 cgctgccgcc tactatgtgg gctacctcca gccccggacc ttcctgctga agtacaacga
     3601 gaatggcacc atcacagacg cagtggattg cgccctggac cccctgagcg agacaaagtg
     3661 tacactgaag tcctttaccg tggagaaggg catctatcag acatccaatt tcagggtgca
     3721 gccaaccgag tctatcgtgc gctttcctaa tatcacaaac ctgtgcccat ttggcgaggt
     3781 gttcaacgca acccgcttcg ccagcgtgta cgcctggaat aggaagcgga tcagcaactg
     3841 cgtggccgac tatagcgtgc tgtacaactc cgcctctttc agcaccttta agtgctatgg
     3901 cgtgtccccc acaaagctga atgacctgtg ctttaccaac gtctacgccg attctttcgt
     3961 gatcaggggc gacgaggtgc gccagatcgc ccccggccag acaggcaaga tcgcagacta
     4021 caattataag ctgccagacg atttcaccgg ctgcgtgatc gcctggaaca gcaacaatct
     4081 ggattccaaa gtgggcggca actacaatta tctgtaccgg ctgtttagaa agagcaatct
     4141 gaagcccttc gagagggaca tctctacaga aatctaccag gccggcagca ccccttgcaa
     4201 tggcgtggag ggctttaact gttatttccc actccagtcc tacggcttcc agcccacaaa
     4261 cggcgtgggc tatcagcctt accgcgtggt ggtgctgagc tttgagctgc tgcacgcccc
     4321 agcaacagtg tgcggcccca agaagtccac caatctggtg aagaacaagt gcgtgaactt
     4381 caacttcaac ggcctgaccg gcacaggcgt gctgaccgag tccaacaaga agttcctgcc
     4441 atttcagcag ttcggcaggg acatcgcaga taccacagac gccgtgcgcg acccacagac
     4501 cctggagatc ctggacatca caccctgctc tttcggcggc gtgagcgtga tcacacccgg
     4561 caccaataca agcaaccagg tggccgtgct gtatcaggac gtgaattgta ccgaggtgcc
     4621 cgtggctatc cacgccgatc agctgacccc aacatggcgg gtgtacagca ccggctccaa
     4681 cgtcttccag acaagagccg gatgcctgat cggagcagag cacgtgaaca attcctatga
     4741 gtgcgacatc ccaatcggcg ccggcatctg tgcctcttac cagacccaga caaactctcc
     4801 cagaagagcc cggagcgtgg cctcccagtc tatcatcgcc tataccatgt ccctgggcgc
     4861 cgagaacagc gtggcctact ctaacaatag catcgccatc ccaaccaact tcacaatctc
     4921 tgtgaccaca gagatcctgc ccgtgtccat gaccaagaca tctgtggact gcacaatgta
     4981 tatctgtggc gattctaccg agtgcagcaa cctgctgctc cagtacggca gcttttgtac
     5041 ccagctgaat agagccctga caggcatcgc cgtggagcag gataagaaca cacaggaggt
     5101 gttcgcccag gtgaagcaaa tctacaagac cccccctatc aaggactttg gcggcttcaa
     5161 tttttcccag atcctgcctg atccatccaa gccttctaag cggagcttta tcgaggacct
     5221 gctgttcaac aaggtgaccc tggccgatgc cggcttcatc aagcagtatg gcgattgcct
     5281 gggcgacatc gcagccaggg acctgatctg cgcccagaag tttaatggcc tgaccgtgct
     5341 gccacccctg ctgacagatg agatgatcgc acagtacaca agcgccctgc tggccggcac
     5401 catcacatcc ggatggacct tcggcgcagg agccgccctc cagatcccct ttgccatgca
     5461 gatggcctat aggttcaacg gcatcggcgt gacccagaat gtgctgtacg agaaccagaa
     5521 gctgatcgcc aatcagttta actccgccat cggcaagatc caggacagcc tgtcctctac
     5581 agccagcgcc ctgggcaagc tccaggatgt ggtgaatcag aacgcccagg ccctgaatac
     5641 cctggtgaag cagctgagca gcaacttcgg cgccatctct agcgtgctga atgacatcct
     5701 gagccggctg gacaaggtgg aggcagaggt gcagatcgac cggctgatca ccggccggct
     5761 ccagagcctc cagacctatg tgacacagca gctgatcagg gccgccgaga tcagggccag
     5821 cgccaatctg gcagcaacca agatgtccga gtgcgtgctg ggccagtcta agagagtgga
     5881 cttttgtggc aagggctatc acctgatgtc cttccctcag tctgccccac acggcgtggt
     5941 gtttctgcac gtgacctacg tgcccgccca ggagaagaac ttcaccacag cccctgccat
     6001 ctgccacgat ggcaaggccc actttccaag ggagggcgtg ttcgtgtcca acggcaccca
     6061 ctggtttgtg acacagcgca atttctacga gccccagatc atcaccacag acaacacctt
     6121 cgtgagcggc aactgtgacg tggtcatcgg catcgtgaac aataccgtgt atgatccact
     6181 ccagcccgag ctggacagct ttaaggagga gctggataag tatttcaaga atcacacctc
     6241 ccctgacgtg gatctgggcg acatcagcgg catcaatgcc tccgtggtga acatccagaa
     6301 ggagatcgac cgcctgaacg aggtggctaa gaatctgaac gagagcctga tcgacctcca
     6361 ggagctgggc aagtatgagc agtacatcaa gtggccctgg tacatctggc tgggcttcat
     6421 cgccggcctg atcgccatcg tgatggtgac catcatgctg tgctgtatga catcctgctg
     6481 ttcttgcctg aagggctgct gtagctgtgg ctcctgctgt aagtttgacg aggatgactc
     6541 tgaacctgtg ctgaagggcg tgaagctgca ttacgactaa atcggatccc gggcccttcg
     6601 aacaaaaact catctcagaa gaggatctga atatgcatac cggtcatcat caccatcacc
     6661 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6721 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6781 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6841 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6901 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     6961 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7021 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7081 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7141 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7201 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7261 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7321 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7381 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7441 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7501 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7561 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7621 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     7681 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     7741 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     7801 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7861 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7921 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7981 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     8041 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     8101 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     8161 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     8221 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     8281 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     8341 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8401 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8461 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8521 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8581 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc
     8641 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     8701 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     8761 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8821 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8881 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     8941 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9001 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9061 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9121 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9301 aaaaggccag gaaccgtaaa aa
//
