LOCUS       sequence-436199-        9322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; 
            Lys1269Ala and His1271Ala substitutions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Dey D, Qing E, He Y, Chen Y, Jennings B, Cohn W, Singh S, Gakhar L, 
            Schnicker NJ, Pierce BG, Whitelegge JP, Doray B, Orban J, Gallagher 
            T, Hasan SS
  TITLE     A single C-terminal residue controls SARS-CoV-2 spike trafficking 
            and incorporation into VLPs.
  JOURNAL   Nat Commun. 2023 Dec 15;14(1):8358. doi: 10.1038/s41467-023-44076-3.
  PUBMED    38102143
REFERENCE   2  (bases 1 to 9322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-12-15"; volume: "14(1):8358. doi"; pages: " 
            10.1038/s41467-023-44076-3"
COMMENT     Sequence Label: SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala
FEATURES             Location/Qualifiers
     source          1..9322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(18..606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(98..117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(777..1568,1569..1637))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1400..1419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1638..1742)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1817..1836)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2008..2387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2388..2591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2542..2562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2636..2655
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2636..2654
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6599..6628
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6644..6661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6684..6701)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6690..6914
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6960..7388
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7047..7066)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7257..7278
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7397..7417)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7402..7731
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7582..7717
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7644..7663
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7798..8592
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7852..7871)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8462..8481
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8768..8889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8805..8824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8859..8878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8938..8954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8938..8954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8951..8973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8962..8978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8986..8992,8993..9010,9011..9016))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9031..9052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9169..9186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
       61 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      121 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      181 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      241 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      301 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      361 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      421 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      481 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      541 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      601 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      661 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      721 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      781 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      841 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      901 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      961 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1021 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1081 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1141 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1201 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1261 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1321 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1381 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1441 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1501 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1561 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1621 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1681 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1741 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     1801 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     1861 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     1921 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     1981 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     2041 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     2101 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2161 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2221 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2281 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     2341 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     2401 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     2461 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2521 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2581 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
     2641 cgactcacta tagggagacc caagctggct agttaagctt ggtaccgagc tcggatccac
     2701 tagtccagtg tggtggaatt cgagctcggt acctcgcgaa tgcatctaga tgccaccatg
     2761 ttcgtcttcc tggtcctgct gcctctggtc tcctcacagt gcgtcaatct gacaactcgg
     2821 actcagctgc cacctgctta tactaatagc ttcaccagag gcgtgtacta tcctgacaag
     2881 gtgtttagaa gctccgtgct gcactctaca caggatctgt ttctgccatt ctttagcaac
     2941 gtgacctggt tccacgccat ccacgtgagc ggcaccaatg gcacaaagcg gttcgacaat
     3001 cccgtgctgc cttttaacga tggcgtgtac ttcgcctcta ccgagaagag caacatcatc
     3061 agaggctgga tctttggcac cacactggac tccaagacac agtctctgct gatcgtgaac
     3121 aatgccacca acgtggtcat caaggtgtgc gagttccagt tttgtaatga tcccttcctg
     3181 ggcgtgtact atcacaagaa caataagagc tggatggagt ccgagtttag agtgtattct
     3241 agcgccaaca actgcacatt tgagtacgtg agccagcctt tcctgatgga cctggagggc
     3301 aagcagggca atttcaagaa cctgagggag ttcgtgttta agaatatcga cggctacttc
     3361 aaaatctact ctaagcacac ccccatcaac ctggtgcgcg acctgcctca gggcttcagc
     3421 gccctggagc ccctggtgga tctgcctatc ggcatcaaca tcacccggtt tcagacactg
     3481 ctggccctgc acagaagcta cctgacaccc ggcgactcct ctagcggatg gaccgccggc
     3541 gctgccgcct actatgtggg ctacctccag ccccggacct tcctgctgaa gtacaacgag
     3601 aatggcacca tcacagacgc agtggattgc gccctggacc ccctgagcga gacaaagtgt
     3661 acactgaagt cctttaccgt ggagaagggc atctatcaga catccaattt cagggtgcag
     3721 ccaaccgagt ctatcgtgcg ctttcctaat atcacaaacc tgtgcccatt tggcgaggtg
     3781 ttcaacgcaa cccgcttcgc cagcgtgtac gcctggaata ggaagcggat cagcaactgc
     3841 gtggccgact atagcgtgct gtacaactcc gcctctttca gcacctttaa gtgctatggc
     3901 gtgtccccca caaagctgaa tgacctgtgc tttaccaacg tctacgccga ttctttcgtg
     3961 atcaggggcg acgaggtgcg ccagatcgcc cccggccaga caggcaagat cgcagactac
     4021 aattataagc tgccagacga tttcaccggc tgcgtgatcg cctggaacag caacaatctg
     4081 gattccaaag tgggcggcaa ctacaattat ctgtaccggc tgtttagaaa gagcaatctg
     4141 aagcccttcg agagggacat ctctacagaa atctaccagg ccggcagcac cccttgcaat
     4201 ggcgtggagg gctttaactg ttatttccca ctccagtcct acggcttcca gcccacaaac
     4261 ggcgtgggct atcagcctta ccgcgtggtg gtgctgagct ttgagctgct gcacgcccca
     4321 gcaacagtgt gcggccccaa gaagtccacc aatctggtga agaacaagtg cgtgaacttc
     4381 aacttcaacg gcctgaccgg cacaggcgtg ctgaccgagt ccaacaagaa gttcctgcca
     4441 tttcagcagt tcggcaggga catcgcagat accacagacg ccgtgcgcga cccacagacc
     4501 ctggagatcc tggacatcac accctgctct ttcggcggcg tgagcgtgat cacacccggc
     4561 accaatacaa gcaaccaggt ggccgtgctg tatcaggacg tgaattgtac cgaggtgccc
     4621 gtggctatcc acgccgatca gctgacccca acatggcggg tgtacagcac cggctccaac
     4681 gtcttccaga caagagccgg atgcctgatc ggagcagagc acgtgaacaa ttcctatgag
     4741 tgcgacatcc caatcggcgc cggcatctgt gcctcttacc agacccagac aaactctccc
     4801 agaagagccc ggagcgtggc ctcccagtct atcatcgcct ataccatgtc cctgggcgcc
     4861 gagaacagcg tggcctactc taacaatagc atcgccatcc caaccaactt cacaatctct
     4921 gtgaccacag agatcctgcc cgtgtccatg accaagacat ctgtggactg cacaatgtat
     4981 atctgtggcg attctaccga gtgcagcaac ctgctgctcc agtacggcag cttttgtacc
     5041 cagctgaata gagccctgac aggcatcgcc gtggagcagg ataagaacac acaggaggtg
     5101 ttcgcccagg tgaagcaaat ctacaagacc ccccctatca aggactttgg cggcttcaat
     5161 ttttcccaga tcctgcctga tccatccaag ccttctaagc ggagctttat cgaggacctg
     5221 ctgttcaaca aggtgaccct ggccgatgcc ggcttcatca agcagtatgg cgattgcctg
     5281 ggcgacatcg cagccaggga cctgatctgc gcccagaagt ttaatggcct gaccgtgctg
     5341 ccacccctgc tgacagatga gatgatcgca cagtacacaa gcgccctgct ggccggcacc
     5401 atcacatccg gatggacctt cggcgcagga gccgccctcc agatcccctt tgccatgcag
     5461 atggcctata ggttcaacgg catcggcgtg acccagaatg tgctgtacga gaaccagaag
     5521 ctgatcgcca atcagtttaa ctccgccatc ggcaagatcc aggacagcct gtcctctaca
     5581 gccagcgccc tgggcaagct ccaggatgtg gtgaatcaga acgcccaggc cctgaatacc
     5641 ctggtgaagc agctgagcag caacttcggc gccatctcta gcgtgctgaa tgacatcctg
     5701 agccggctgg acaaggtgga ggcagaggtg cagatcgacc ggctgatcac cggccggctc
     5761 cagagcctcc agacctatgt gacacagcag ctgatcaggg ccgccgagat cagggccagc
     5821 gccaatctgg cagcaaccaa gatgtccgag tgcgtgctgg gccagtctaa gagagtggac
     5881 ttttgtggca agggctatca cctgatgtcc ttccctcagt ctgccccaca cggcgtggtg
     5941 tttctgcacg tgacctacgt gcccgcccag gagaagaact tcaccacagc ccctgccatc
     6001 tgccacgatg gcaaggccca ctttccaagg gagggcgtgt tcgtgtccaa cggcacccac
     6061 tggtttgtga cacagcgcaa tttctacgag ccccagatca tcaccacaga caacaccttc
     6121 gtgagcggca actgtgacgt ggtcatcggc atcgtgaaca ataccgtgta tgatccactc
     6181 cagcccgagc tggacagctt taaggaggag ctggataagt atttcaagaa tcacacctcc
     6241 cctgacgtgg atctgggcga catcagcggc atcaatgcct ccgtggtgaa catccagaag
     6301 gagatcgacc gcctgaacga ggtggctaag aatctgaacg agagcctgat cgacctccag
     6361 gagctgggca agtatgagca gtacatcaag tggccctggt acatctggct gggcttcatc
     6421 gccggcctga tcgccatcgt gatggtgacc atcatgctgt gctgtatgac atcctgctgt
     6481 tcttgcctga agggctgctg tagctgtggc tcctgctgta agtttgacga ggatgactct
     6541 gaacctgtgc tgaagggcgt ggcactggca tacacctaaa tcggatcccg ggcccttcga
     6601 acaaaaactc atctcagaag aggatctgaa tatgcatacc ggtcatcatc accatcacca
     6661 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     6721 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     6781 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     6841 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     6901 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     6961 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7021 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7081 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     7141 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     7201 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     7261 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     7321 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     7381 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     7441 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     7501 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     7561 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7621 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     7681 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     7741 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     7801 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     7861 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     7921 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     7981 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     8041 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     8101 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     8161 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     8221 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     8281 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     8341 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     8401 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     8461 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     8521 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     8581 gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct
     8641 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8701 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8761 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8821 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8881 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8941 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     9001 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     9061 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     9121 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9301 aaaggccagg aaccgtaaaa ag
//
