LOCUS       sequence-43623-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA7a-RFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    193..209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(213..232)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(214..232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(320..339)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        545..622
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(642..661)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cctttggtaa ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca
       61 ccttcgattt cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg
      121 aactctttga taacgtcttc gctactcgcc atatgtatat ctccttctta aaagatcttt
      181 tgaattctga aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg
      241 ggatcgagat cgatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
      301 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
      361 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
      421 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
      481 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat
      541 cccggacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
      601 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
      661 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
      721 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
      781 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
      841 gcacgcgccg
//
