LOCUS       sequence-43625-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA7c-RFP
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    192..208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(212..231)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(213..231)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(319..338)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        544..621
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(641..660)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ctttggtaac tttcagttta gcggtctggg taccttcgta cggacgacct tcaccttcac
       61 cttcgatttc gaactcgtga ccgttaacgg aaccttccat acgaactttg aaacgcatga
      121 actctttgat aacgtcttcg ctactcgcca tatgtatatc tccttcttaa aagatctttt
      181 gaattctgaa attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg
      241 gatcgagatc gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
      301 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
      361 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
      421 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
      481 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc
      541 ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag
      601 agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc
      661 ggtgtctctt atcagaccgt
//
