LOCUS       sequence-43627-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA7k-RFP
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(201..220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(202..220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(308..327)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        533..610
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(630..649)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc ttcgatttcg
       61 aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa ctctttgata
      121 acgtcttcgc tactcgccat atgtatatct ccttcttaaa agatcttttg aattctgaaa
      181 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatcg
      241 atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
      301 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
      361 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
      421 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
      481 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat
      541 cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
      601 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      661 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      721 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      781 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      841 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
//
