LOCUS       sequence-436288-        9025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses of GFP-tagged mTet1s_K852E in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, 
            Ludwig AK, Carell T, Cardoso MC
  TITLE     Isoform-specific and ubiquitination dependent recruitment of Tet1 to
            replicating heterochromatin modulates methylcytosine oxidation.
  JOURNAL   Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.
  PUBMED    36056023
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-2"; volume: "13(1):5173. doi"; pages: " 
            10.1038/s41467-022-32799-8"
COMMENT     Sequence Label: pGFP-mTet1s_K852E  pc3915
FEATURES             Location/Qualifiers
     source          1..9025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1793..1811
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1849..1871)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1971..1990
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2057..2512)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2194..2215)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2406..2425
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2638..2660
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2652..2669
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2653..2669
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2676..2694
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        2763..3066
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3068..3345
                     /label=chicken beta-actin promoter
     intron          3346..4362
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4286..4308
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4370..4389
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4434..4443
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4440..4442,4443..4445,4446..5156)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4440..4442,4443..4445,4446..5156)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4485..4506)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4746..4765)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5093..5114
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5163..5183
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_feature    7416..7878
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7472..7489)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7699..7718
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      7883..7892
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7889..8287
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8445..8566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8482..8501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8536..8555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8614..8749
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8855..8872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1801 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     1861 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     1921 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     1981 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     2041 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     2101 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     2161 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     2221 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     2281 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     2341 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     2401 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     2461 ctgcgcgtaa ccaccacacc cgccgcgctc aatgcgccgc tacagggcgc gtccattcgc
     2521 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2581 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2641 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta taggacgcgt
     2701 cctagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2761 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2821 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2881 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2941 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     3001 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     3061 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     3121 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     3181 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
     3241 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
     3301 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc
     3361 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
     3421 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     3481 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
     3541 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
     3601 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
     3661 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     3721 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
     3781 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
     3841 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
     3901 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     3961 ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
     4021 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     4081 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     4141 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     4201 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     4261 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     4321 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     4381 gttattgtgc tgtctcatca ttttggcaaa gaattcggat ccaccggccg gtcgccacca
     4441 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4501 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4561 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4621 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     4681 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4741 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4801 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4861 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4921 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4981 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     5041 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     5101 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg
     5161 gagaaaacct atacttccaa ggaagatctc gaggcgatcg catggaagct gcaccctgtg
     5221 actgtgatgg aggtacacaa aaagaaaaag gcccatatta tacacacctt ggggcaggac
     5281 caagtgtggc tgctgtcagg gagctcatgg agactaggtt tggccagaag gggaaggcaa
     5341 tccggattga gaagatagtg ttcacgggga aggaagggaa gagctctcag ggctgcccgg
     5401 tcgccaagtg ggtgatcaga agaagtggtc ctgaagagaa gcttatttgt ttggttcgtg
     5461 agcgtgtaga ccatcactgt tcgacggctg tgatagttgt ccttatcctg ctgtgggaag
     5521 gtatccctcg cctgatggct gaccgcctgt acaaagagct cactgagaac ttgaggtcct
     5581 acagcggaca tcccacagac cgaagatgta ccctcaacaa aaagcgtacc tgcacctgtc
     5641 aaggcatcga cccaaaaacc tgcggagcgt ccttctcctt tggctgttcg tggagcatgt
     5701 atttcaacgg ctgtgagttt gggaggagtg aaaaccccag aaaattcaga cttgctccaa
     5761 actacccctt acataactac tataagagaa ttactggaat gagttctgaa ggaagtgacg
     5821 tgaaaaccgg gtggatcatt ccagaccgca agaccctcat aagcagagag gaaaaacagc
     5881 ttgaaaagaa tttacaagaa ttggctacag tattagctcc actttacaag cagatggctc
     5941 cagttgctta tcaaaatcag gtggaatatg aagaagttgc tggagactgt cgacttggaa
     6001 atgaagaggg gcgtcctttc tctggtgtca cctgttgcat ggatttttgt gcccattctc
     6061 acaaggacat tcacaacatg cacaacggaa gcaccgtggt gtgtacgttg attcgagcag
     6121 atggccgtga cacaaattgt cccgaggatg aacaactcca cgtcctgcca ctataccggc
     6181 ttgcagacac tgatgaattt ggctccgtgg aagggatgaa ggccaaaatc aaatctgggg
     6241 ccatccaagt caatgggcca accaggaaga ggcgactacg ttttactgag cctgttcctc
     6301 gatgtgggaa gagggccaaa atgaagcaga accacaataa atcaggtact gctggtctca
     6361 gaaggaagag gatctctgcc agcccaaagg gagccccagg ttcacacaac actaagagct
     6421 tttcatcagc ctcatctact tctcacctag tgaaagacga atctacagac ttctgtcccc
     6481 tgcaggcttc ctccgcagaa acatctacct gtacgtacag taaaacagcc tcaggtgggt
     6541 ttgcagaaac aagtagtatt ctccactgca caatgccttc tggagcacac agtggtgcta
     6601 atgcagctgc tggggaatgt actggaacgg tgcagcctgc cgaggtggct gctcatcctc
     6661 accagtctct tcccacagcc gattctcccg ttcatgctga gcctctcact agtccatctg
     6721 agcagctaac ttctaaccag tcaaaccagc agctccctct cctcagcaat tctcagaaac
     6781 tggcttcctg tcaggtggaa gatgagcggc accctgaagc ggatgagcct cagcaccccg
     6841 aggacgataa cttgcctcaa cttgatgaat tctggtcaga cagtgaggag atctacgccg
     6901 atccttcctt tggtggcgtg gcgatagcac ccattcacgg ctcggtgctc attgagtgcg
     6961 ctcggaagga gcttcatgct accacctctt tgcgctcccc caaacgaggg gtcccttttc
     7021 gtgtgtccct tgtattctac cagcacaaaa gcctaaacaa gcctaatcat ggttttgata
     7081 tcaacaaaat taagtgtaaa tgcaaaaaag taacgaaaaa aaagcccgca gaccgggagt
     7141 gtcctgatgt atcccccgaa gccaatttat cacaccaaat tccttctcga gttgcatcaa
     7201 ccttaacccg agacaatgtt gttaccgtgt ccccatactc tctcactcat gttgcgggac
     7261 cctacaatcg ttgggtctaa gcggccgcag ttaacgaatt ccgccccccc cccctccccc
     7321 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     7381 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     7441 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     7501 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     7561 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     7621 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     7681 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     7741 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     7801 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     7861 tgaaaaacac gatgataagc ttgccaccat ggccaagcct ttgtctcaag aagaatccac
     7921 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     7981 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     8041 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     8101 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     8161 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     8221 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     8281 gggctaagca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     8341 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     8401 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     8461 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8521 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     8581 cgtcgacctc gaggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cttcactact
     8641 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     8701 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     8761 ggggcgggac tatggttgct gactaattgc tgcattaatg aatcggccaa cgcgcgggga
     8821 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8881 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8941 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9001 gtaaaaaggc cgcgttgctg gcgtt
//
