LOCUS       sequence-436290-        1281 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of TwinStrep-Flag -ATG13 C-terminal and ULK1 
            MIT domain fusion complex fused with GFP-10His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)-msfGFP
FEATURES             Location/Qualifiers
     source          1..1281
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..39
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             76..99
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             136..156
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gccaccatgg cggcctggtc acacccgcag ttcgagaaag gtggaggtgc ccgaggtgga
       61 tcgggaggtg gatcgtggag ccacccgcag ttcgaaaaag gattcgatta caaggatgac
      121 gatgacaagg gtaccgaaaa cctgtacttc caatcccacg atgtcctgga aaccatcttc
      181 gtgagaaaag tcggcgcttt tgtgaacaag cccattaatc aggtcacact cacctctctg
      241 gacatccctt tcgctatgtt tgcaccaaag aacctcgagc tggaagacac tgatcccatg
      301 gtgaatcctc cagactcacc cgagacagaa agtcctctcc agggcagtct gcacagcgat
      361 ggaagctccg gtggctcttc aggcaacaca catgacgatt tcgtcatgat tgacttcaaa
      421 cccgcttttt ccaaggacga tatcctccct atggatctgg gaaccttcta cagggagttt
      481 cagaatcccc ctcagctcag tagcctgagc attgacatcg gtgcccagtc catggctgag
      541 gacctcgatt ctctgcctga aaaactggct gtgcacgaga agaacgtcag agaattcgac
      601 gcatttgtgg agaccctgca gggtagcgac gaagcgatgg agcaggaaca cactgagatc
      661 ctgaggggtc tcagattcac cctgctcttt gtgcagcatg tcctggaaat tgctgcactc
      721 aagggtagtg ctagcgaggc agctggtgga ccagaatacc agctgcagga gagcgtggtc
      781 gccgaccaga tctctctgct ctcaagggaa tggggcttcg ctgagcagct ggtcctctat
      841 ctgaaggtgg ccgaactgct cagtagcggc ctgcagtccg ccatcgatca gattagagct
      901 ggaaagctct gcctgtcctc tactgtgaaa caggtggtcc ggcgcctgaa cgagctctac
      961 aaggccagtg tggtcagctg tcagggactc tctctgcgtc tccagcggtt ctttctggac
     1021 aaacagcgcc tgctcgatag gatccactca attacagctg aacgcctgat cttcagccat
     1081 gcagtgcaga tggtccagtc cgcagccctg gacgaaatgt ttcagcacag ggagggatgc
     1141 gtccccagat accataaggc tctgctcctg ctcgagggtc tgcagcacat gctcagcgac
     1201 caggccgata tcgaaaatgt gactaagtgc aaactgtgta ttgagaggag actctccgct
     1261 ctgctcacag gcatctgtgc a
//
