LOCUS       sequence-436297-        1152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain 
            fusion complex -6His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: MBP-ATG13 (363-517)-ULK1 (836-1050)-His
FEATURES             Location/Qualifiers
     source          1..1152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1132..1149
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cacgatgtcc tggaaaccat cttcgtgaga aaagtcggcg cttttgtgaa caagcccatt
       61 aatcaggtca cactcacctc tctggacatc cctttcgcta tgtttgcacc aaagaacctc
      121 gagctggaag acactgatcc catggtgaat cctccagact cacccgagac agaaagtcct
      181 ctccagggca gtctgcacag cgatggaagc tccggtggct cttcaggcaa cacacatgac
      241 gatttcgtca tgattgactt caaacccgct ttttccaagg acgatatcct ccctatggat
      301 ctgggaacct tctacaggga gtttcagaat ccccctcagc tcagtagcct gagcattgac
      361 atcggtgccc agtccatggc tgaggacctc gattctctgc ctgaaaaact ggctgtgcac
      421 gagaagaacg tcagagaatt cgacgcattt gtggagaccc tgcagggtag cgacgaagcg
      481 atggagcagg aacacactga gatcctgagg ggtctcagat tcaccctgct ctttgtgcag
      541 catgtcctgg aaattgctgc actcaagggt agtgctagcg aggcagctgg tggaccagaa
      601 taccagctgc aggagagcgt ggtcgccgac cagatctctc tgctctcaag ggaatggggc
      661 ttcgctgagc agctggtcct ctatctgaag gtggccgaac tgctcagtag cggcctgcag
      721 tccgccatcg atcagattag agctggaaag ctctgcctgt cctctactgt gaaacaggtg
      781 gtccggcgcc tgaacgagct ctacaaggcc agtgtggtca gctgtcaggg actctctctg
      841 cgtctccagc ggttctttct ggacaaacag cgcctgctcg ataggatcca ctcaattaca
      901 gctgaacgcc tgatcttcag ccatgcagtg cagatggtcc agtccgcagc cctggacgaa
      961 atgtttcagc acagggaggg atgcgtcccc agataccata aggctctgct cctgctcgag
     1021 ggtctgcagc acatgctcag cgaccaggcc gatatcgaaa atgtgactaa gtgcaaactg
     1081 tgtattgaga ggagactctc cgctctgctc acaggcatct gtgcactcga gcaccaccac
     1141 caccaccact ga
//
