LOCUS       sequence-436298-        1194 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain 
            fusion complex -6His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: MBP-ATG13 (450-517)-ULK1 (836-1050)-His
FEATURES             Location/Qualifiers
     source          1..1194
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1098
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1072..1095
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1162..1182
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc
       61 gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg
      121 gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc
      181 ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc
      241 ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac
      301 ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat
      361 ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa
      421 gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg
      481 attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac
      541 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa
      601 aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc
      661 gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg
      721 aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc
      781 gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc
      841 gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt
      901 gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccact
      961 atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg
     1021 tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc
     1081 ctgaaagacg cgcagactaa ttcgagctcg aacaacaaca acaataacaa taacaacaac
     1141 ctcggggata tcccaacgac cgaaaacctg tattttcagg gcgccatggg atcc
//
