LOCUS       sequence-436299-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain 
            fusion complex -6His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: MBP-ATG13 (450-517)-ULK1 (836-1050)-His
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             871..888
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaacccgctt tttccaagga cgatatcctc cctatggatc tgggaacctt ctacagggag
       61 tttcagaatc cccctcagct cagtagcctg agcattgaca tcggtgccca gtccatggct
      121 gaggacctcg attctctgcc tgaaaaactg gctgtgcacg agaagaacgt cagagaattc
      181 gacgcatttg tggagaccct gcagggtagc gacgaagcga tggagcagga acacactgag
      241 atcctgaggg gtctcagatt caccctgctc tttgtgcagc atgtcctgga aattgctgca
      301 ctcaagggta gtgctagcga ggcagctggt ggaccagaat accagctgca ggagagcgtg
      361 gtcgccgacc agatctctct gctctcaagg gaatggggct tcgctgagca gctggtcctc
      421 tatctgaagg tggccgaact gctcagtagc ggcctgcagt ccgccatcga tcagattaga
      481 gctggaaagc tctgcctgtc ctctactgtg aaacaggtgg tccggcgcct gaacgagctc
      541 tacaaggcca gtgtggtcag ctgtcaggga ctctctctgc gtctccagcg gttctttctg
      601 gacaaacagc gcctgctcga taggatccac tcaattacag ctgaacgcct gatcttcagc
      661 catgcagtgc agatggtcca gtccgcagcc ctggacgaaa tgtttcagca cagggaggga
      721 tgcgtcccca gataccataa ggctctgctc ctgctcgagg gtctgcagca catgctcagc
      781 gaccaggccg atatcgaaaa tgtgactaag tgcaaactgt gtattgagag gagactctcc
      841 gctctgctca caggcatctg tgcactcgag caccaccacc accaccactg a
//
