LOCUS       sequence-436300-        9284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses of mcherry-tagged mTet1-ZF-CD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, 
            Ludwig AK, Carell T, Cardoso MC
  TITLE     Isoform-specific and ubiquitination dependent recruitment of Tet1 to
            replicating heterochromatin modulates methylcytosine oxidation.
  JOURNAL   Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.
  PUBMED    36056023
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-2"; volume: "13(1):5173. doi"; pages: " 
            10.1038/s41467-022-32799-8"
COMMENT     Sequence Label: pmch-mTet1-ZF-CD  pc3956
FEATURES             Location/Qualifiers
     source          1..9284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      866..1001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1049..1170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1060..1079)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1114..1133
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1328..1726)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      1723..1732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1737..2310
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1897..1916)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2126..2143
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(4439..4459)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(4472..4492)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      4684..4693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4739..5446)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5014..5033)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5283..5301
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      5443..5452
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5487..5506)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5514..6530
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5568..5590)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6531..6806)
                     /label=chicken beta-actin promoter
     enhancer        6808..7111
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(7180..7198)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7205..7222)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7205..7221)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7214..7236)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7362..7817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7449..7468)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7659..7680
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7884..7903)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8003..8025
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8063..8081)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8149..8253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8254..8322,8323..9114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8472..8491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaatt agtcagcaac
      841 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
      901 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
      961 tgagctattc cagaagtagt gaagaggctt ttttggaggc ctaggctttt gcaaaaagct
     1021 cctcgaggtc gacggtatac agacatgata agatacattg atgagtttgg acaaaccaca
     1081 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1141 gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca gcatgagatc
     1201 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     1261 atagaaggcg gcggtggaat cgaaatctcg tagcacgtgt cagtcctgct cctcggccac
     1321 gaagtgctta gccctcccac acataaccag agggcagcaa ttcacgaatc ccaactgccg
     1381 tcggctgtcc atcactgtcc ttcactatgg ctttgatccc aggatgcaga tcgagaagca
     1441 cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg atcgcgacga
     1501 tacaagtcag gttgccagct gccgcagcag cagcagtgcc cagcaccacg agttctgcac
     1561 aaggtccccc agtaaaatga tatacattga caccagtgaa gatgcggccg tcgctagaga
     1621 gagctgcgct ggcgacgctg tagtcttcag agatggggat gctgttgatt gtagccgttg
     1681 ctctttcaat gagggtggat tcttcttgag acaaaggctt ggccatggtg gcaagcttat
     1741 catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag acgttttttt
     1801 aacctcgact aaacacatgt aaagcatgtg caccgaggcc ccagatcaga tcccatacaa
     1861 tggggtacct tctgggcatc cttcagcccc ttgttgaata cgcttgagga gagccatttg
     1921 actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc ctttgcaggt
     1981 gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg ggggttccgc
     2041 tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct
     2101 tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga
     2161 atgctcgtca agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat
     2221 atggtggaaa ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca
     2281 gtaacgttag ggggggggga gggagagggg gggggggggg gcggaattcg ttaactgcgg
     2341 ccgcttagac ccaacgattg tagggtcccg caacatgagt gagagagtat ggggacacgg
     2401 taacaacatt gtctcgggtt aaggttgatg caactcgaga aggaatttgg tgtgataaat
     2461 tggcttcggg ggatacatca ggacactccc ggtctgcggg cttttttttc gttacttttt
     2521 tgcatttaca cttaattttg ttgatatcaa aaccatgatt aggcttgttt aggcttttgt
     2581 gctggtagaa tacaagggac acacgaaaag ggacccctcg tttgggggag cgcaaagagg
     2641 tggtagcatg aagctccttc cgagcgcact caatgagcac cgagccgtga atgggtgcta
     2701 tcgccacgcc accaaaggaa ggatcggcgt agatctcctc actgtctgac cagaattcat
     2761 caagttgagg caagttatcg tcctcggggt gctgaggctc atccgcttca gggtgccgct
     2821 catcttccac ctgacaggaa gccagtttct gagaattgct gaggagaggg agctgctggt
     2881 ttgactggtt agaagttagc tgctcagatg gactagtgag aggctcagca tgaacgggag
     2941 aatcggctgt gggaagagac tggtgaggat gagcagccac ctcggcaggc tgcaccgttc
     3001 cagtacattc cccagcagct gcattagcac cactgtgtgc tccagaaggc gttgtgcagt
     3061 ggagaatact acttgtttct gcaaacccac ctgaggctgt tttactgtac gtacaggtag
     3121 atgtttctgc ggaggaagcc tgcaggggac agaagtctgt agattcgtct ttcactaggt
     3181 gagaagtaga tgaggctgat gaaaagctct tagtgttgtg tgaacctggg gctccctttg
     3241 ggctggcaga gatcctcttc cttctgagac cagcagtacc tgatttattg tggttctgct
     3301 tcattttggc cctcttccca catcgaggaa caggctcagt aaaacgtagt cgcctcttcc
     3361 tggttggccc attgacttgg atggccccag atttgatttt ggccttcatc ccttccacgg
     3421 agccaaattc atcagtgtct gcaagccggt atagtggcag gacgtggagt tgttcatcct
     3481 cgggacaatt tgtgtcacgg ccatctgctc gaatcaacgt acacaccacg gtgcttccgt
     3541 tgtgcatgtt gtgaatgtcc ttgtgagaat gggcacaaaa atccatgcaa caggtgacac
     3601 cagagaaagg acgcccctct tcatttccaa gtcgacagtc tccagcaact tcttcatatt
     3661 ccacctgatt ttgataagca actggagcca tctgcttgta aagtggagct aatactgtag
     3721 ccaattcttg taaattcttt tcaagctgtt tttcctctct gcttatgagg gtcttgcggt
     3781 ctggaatgat ccacccggtt ttcacgtcac ttccttcaga actcattcca gtaattctct
     3841 tatagtagtt atgtaagggg tagtttggag caagtctgaa ttttctgggg ttttcactcc
     3901 tcccaaactt acagccgttg aaatacatgc tccacgaaca gccaaaggag aaggacgctc
     3961 cgcaggtttt tgggtcgatg ccttgacagg tgcaggtacg ctttttgttg agggtacatc
     4021 ttcggtctgt gggatgtccg ctgtaggacc tcaagttctc agtgagctct ttgtacaggc
     4081 ggtcagccat caggcgaggg ataccttccc acagcaggat aaggacaact atcacagccg
     4141 tcgaacagtg atggtctaca cgctcacgaa ccaaacaaat aagcttctct tcaggaccac
     4201 ttcttctgat cacccacttg gcgaccgggc agccctgaga gctcttccct tccttccccg
     4261 tgaacactat cttctcaatc cggattgcct tccccttctg gccaaaccta gtctccatga
     4321 gctccctgac agcagccaca cttggtcctg ccccaaggtg tgtataatat gggccttttt
     4381 ctttttgtgt acctccatca cagtcacagg gtgcagcttc catgcgatcg caagagcctc
     4441 cttggaagta taggttttcg cttccgcccc gtccttggaa gtataggttt tctccggatc
     4501 cgccgcggcc gcctggcttc tttttgagca cctcgcattt cctcttctta cagatctggt
     4561 ggctgttctt tcgattcttg cagtaggtgc actccccaca gttggccttc tgctggcacg
     4621 gctcacagac cccacaggcc ttgcgcttcc tcctctccac cattggcggc gtagagcgat
     4681 cgcccatggt ggcgtccacg gcgctgttgc tcgcctgggc tctgctcctc aggccgctct
     4741 tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg tactgttcca
     4801 cgatggtgta gtcctcgttg tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc
     4861 cgggcagctg cacgggcttc ttggccttgt aggtggtctt gacctcagcg tcgtagtggc
     4921 cgccgtcctt cagcttcagc ctctgcttga tctcgccctt cagggcgccg tcctcggggt
     4981 acatccgctc ggaggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg
     5041 aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg
     5101 agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga
     5161 agccctcggg gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg
     5221 ccttggagcc gtacatgaac tgaggggaca ggatgtccca ggcgaagggc agggggccac
     5281 ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc
     5341 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
     5401 actccttgat gatggccatg ttatcctcct cgcccttgct caccatggtg gcgggatccg
     5461 aattctttgc caaaatgatg agacagcaca ataaccagca cgttgcccag gagctgtagg
     5521 aaaaagaaga aggcatgaac atggttagca gaggctctag agccgccggt cacacgccag
     5581 aagccgaacc ccgccctgcc ccgtcccccc cgaaggcagc cgtccccccg cggacagccc
     5641 cgaggctgga gagggagaag gggacggcgg cgcggcgacg cacgaaggcc ctccccgccc
     5701 atttccttcc tgccggcgcc gcaccgcttc gccccgcgcc cgctagaggg ggtgcggcgg
     5761 cgcctcccag atttcggctc cgcacagatt tgggacaaag gaagtccctg cgccctctcg
     5821 cacgattacc ataaaaggca atggctgcgg ctcgccgcgc ctcgacagcc gccggcgctc
     5881 cgggggccgc cgcgcccctc ccccgagccc tccccggccc gaggcggccc cgccccgccc
     5941 ggcaccccca cctgccgcca ccccccgccc ggcacggcga gccccgcgcc acgccccgta
     6001 cggagccccg cacccgaagc cgggccgtgc tcagcaactc ggggaggggg gtgcaggggg
     6061 ggttgcagcc cgaccgacgc gcccacaccc cctgctcacc cccccacgca cacaccccgc
     6121 acgcagcctt tgttcccctc gcagcccccc ccgcaccgcg gggcaccgcc cccggccgcg
     6181 ctcccctcgc gcacactgcg gagcgcacaa agccccgcgc cgcgcccgca gcgctcacag
     6241 ccgccgggca gcgcggagcc gcacgcggcg ctccccacgc acacacacac gcacgcaccc
     6301 cccgagccgc tccccccgca caaagggccc tcccggagcc cctcaaggct ttcacgcagc
     6361 cacagaaaag aaacaagccg tcattaaacc aagcgctaat tacagcccgg aggagaaggg
     6421 ccgtcccgcc cgctcacctg tgggagtaac gcggtcagtc agagccgggg cgggcggcgc
     6481 gaggcggcgg cggagcgggg cacggggcga aggcagcgcg cagcgactcc cgcccgccgc
     6541 gcgcttcgct ttttataggg ccgccgccgc cgccgcctcg ccataaaagg aaactttcgg
     6601 agcgcgccgc tctgattggc tgccgccgca cctctccgcc tcgccccgcc ccgcccctcg
     6661 ccccgccccg ccccgcctgg cgcgcscccc cccccccccc gcccccatcg ctgcacaaaa
     6721 taattaaaaa ataaataaat acaaaattgg gggtggggag gggggggaga tggggagagt
     6781 gaagcagaac gtggggctca cctcgaccat ggtaatagcg atgactaata cgtagatgta
     6841 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     6901 accgtcattg acatcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     6961 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     7021 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     7081 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     7141 tgaccccgta attgattact attaataact aggacgcgtc ctatagtgag tcgtattaca
     7201 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7261 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7321 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
     7381 attgagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7441 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7501 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     7561 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7621 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7681 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt taccgatttc
     7741 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     7801 attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     7861 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     7921 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     7981 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     8041 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     8101 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     8161 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8221 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8281 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8341 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8401 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8461 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     8521 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8581 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8641 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8701 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8761 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8821 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8881 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8941 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9001 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9061 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9121 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9181 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9241 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
