LOCUS       sequence-436303-       11480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses of mcherry-tagged mVprBP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11480)
  AUTHORS   Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, 
            Ludwig AK, Carell T, Cardoso MC
  TITLE     Isoform-specific and ubiquitination dependent recruitment of Tet1 to
            replicating heterochromatin modulates methylcytosine oxidation.
  JOURNAL   Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.
  PUBMED    36056023
REFERENCE   2  (bases 1 to 11480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-2"; volume: "13(1):5173. doi"; pages: " 
            10.1038/s41467-022-32799-8"
COMMENT     Sequence Label: pmcherry-mVprBP  pc2954
FEATURES             Location/Qualifiers
     source          1..11480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      866..1001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1049..1170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1060..1079)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1114..1133
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1328..1726)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      1723..1732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1737..2199
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1897..1916)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2126..2143
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             5649..5660
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      6877..6886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(6932..7639)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7207..7226)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7476..7494
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      7636..7645
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7680..7699)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7707..8723
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7761..7783)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(8724..9002)
                     /label=chicken beta-actin promoter
     enhancer        9004..9307
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(9376..9394)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9401..9418)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9401..9417)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9410..9432)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9558..10013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9645..9664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9855..9876
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10080..10099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10199..10221
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10259..10277)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10345..10449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10450..10518,10519..11310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10668..10687)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaatt agtcagcaac
      841 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
      901 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
      961 tgagctattc cagaagtagt gaagaggctt ttttggaggc ctaggctttt gcaaaaagct
     1021 cctcgaggtc gacggtatac agacatgata agatacattg atgagtttgg acaaaccaca
     1081 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1141 gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca gcatgagatc
     1201 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     1261 atagaaggcg gcggtggaat cgaaatctcg tagcacgtgt cagtcctgct cctcggccac
     1321 gaagtgctta gccctcccac acataaccag agggcagcaa ttcacgaatc ccaactgccg
     1381 tcggctgtcc atcactgtcc ttcactatgg ctttgatccc aggatgcaga tcgagaagca
     1441 cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg atcgcgacga
     1501 tacaagtcag gttgccagct gccgcagcag cagcagtgcc cagcaccacg agttctgcac
     1561 aaggtccccc agtaaaatga tatacattga caccagtgaa gatgcggccg tcgctagaga
     1621 gagctgcgct ggcgacgctg tagtcttcag agatggggat gctgttgatt gtagccgttg
     1681 ctctttcaat gagggtggat tcttcttgag acaaaggctt ggccatggtg gcaagcttat
     1741 catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag acgttttttt
     1801 aacctcgact aaacacatgt aaagcatgtg caccgaggcc ccagatcaga tcccatacaa
     1861 tggggtacct tctgggcatc cttcagcccc ttgttgaata cgcttgagga gagccatttg
     1921 actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc ctttgcaggt
     1981 gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg ggggttccgc
     2041 tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct
     2101 tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga
     2161 atgctcgtca agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat
     2221 atggtggaaa ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca
     2281 gtaacgttag gggggggggg grrttgatcc cgctcgagtt vddcasmrkt cgttaactgc
     2341 ggccgcatca ctcattcaga gataagatga tgtcatcttc caaatcagag ttgtccgagc
     2401 tgtctgtgtc ccccaggatc agctccacct cctcatctgc atcagagtct tcatcttccc
     2461 cctcctctcc ctcctctaga aggtttgcta gctcctcatc agagggagag aagtcattgt
     2521 ctccatcctc tcctgcattc tcattgttgt catcctcctc cagttctgcc tccagcaact
     2581 ggtcagtgtc tagttcatct aaatcatcag tatcatcatc atcttcatca tcatcttctt
     2641 cctcctgttc ttcctcttcc tggtcctcct cttcatcctc atcctctgcc agacgctgcc
     2701 tgcccacttc atagagcctg cacactgtgt ccatattcag tgcatccatg ctgccttgat
     2761 tctcaatgac agcaagataa cagtctttgg tgtctgtgca caggtcaaag atgtttcgtt
     2821 tcacatcaat agttgctata ggtttataat cagttgcgtt gaatgttcga aaggatgacc
     2881 caaaagggct tttcatcctc tcttccagta agtcatcttc atcatctgcc tgcaacattg
     2941 ctccatacat cactgtccca gtgtggttaa aaacaacacg gcattgatcc agagcaggaa
     3001 cagtgtgtaa aagatggaaa gtccgaaggt cccaaatctc tgtattgatg atgacttcca
     3061 gcccatttgg atggaaaaca ccactgatat tcatgttgaa cttgtcaaac ttgtggatgg
     3121 cctgtgcaga gcggacatcc cagaggacgc catcatttaa gacaagatca tctgtaggat
     3181 taaaggtggc acagttcctc ttgtagttgt tggcaagatc tgggttaaac agagtcaaca
     3241 gcttgttgcc agtctgaata tcataaatat gggcaatgtc tccttttgtg cctatgaccc
     3301 gatcctgaga gtgcttactg aactcaacat agtgatcttc tgtgaaggaa tgcttcatat
     3361 caaatactga tttcattccc caaagtgcag acaaaggctg gctccaagtg gcagatgtca
     3421 gaagtaagga cccatccctt gacggttcaa ggtgtgtgat ggctgagttg tgacagttat
     3481 agctggcctc ttcctgtcca ctaaacacat tgtagagctt cagctgccct gtacaagtgc
     3541 caagcatcag gaaccgctcc cgggctgaga atgcacaaca ggtgaagcca ctttcatctt
     3601 cattagcttc ccggaatact gaaatcggac gaaatctgct aaagataagg tgcctatcaa
     3661 agcatccgcc atctacccct ccatattttg gaaatgatgc cctgcggttt agccttgagg
     3721 taaagtttat tggcgcttgc cgcctctgtt ttggttcagg acactgatga ggagtaaaga
     3781 gagaaaaagg aggacaggtg gcaacggggt tcttgcatcg agcatgttgc tctctaagat
     3841 actctgtgat gatactgtcc agcgtaggtg gggaaggaag atgtctatcc aattgttttt
     3901 ttatggcagg gctctggctg tatgcaccat ggtccgactt ctgccgcaac actcgaattt
     3961 tcctgccatt gcacggtgat ggcctctctc tgataaaact gatcctacca atcaaagggg
     4021 aattgcctgc atatgatggg ccaggcaaag gcaatgaacc ctgggggggc cgtggtggag
     4081 gatgggcagg gggagcagaa ggggcagaag cacccacagt agcatggctg cccagtcgac
     4141 ttgcaatgcc gttggcaatc cgaggggtcc ggggaagaga gacaggagaa gcagcagcag
     4201 tcactggggt gaaggcagaa gaatgtgagg cagcagtcat gggcaggtca gcctctctcg
     4261 tcagcacagt tgctgtctct ccaagccctt tagaaatgag atggttccgt atcaacagga
     4321 gcagctcttt ctcagggaag gagatcctcg actgagcaac aacatctgct ttctgcagtc
     4381 gtgcgagaga cacatcagtg ccaatgagaa gaggctttcc tgatacacgc tcaatgagct
     4441 cagcagcgta tttgcagaac ttgacatggt cactacgctt atcctgcagc acaggctctt
     4501 tcatcagctg ttgaatctgg cagctactaa aaaggggaag tttactgatg atctgtcgaa
     4561 cagtgctact tcgagacagg cctacaaggg ctttgcaggc cagggcccgg atctggtctg
     4621 cgtctgtaat gggcatcttg atggacaaca gggacagaag caccttgatg ccattgttgg
     4681 actggaccac attccacatc ttggccagag tatgctcact actcttggga gtctgaggca
     4741 gctttctccg aggagttcca gaaataaatt tgccaatact ggatattcgg ttatctggac
     4801 cacatacaca attgatgata atttgaagtg ctgacttctg aatttcagca tcatggataa
     4861 agaactcacc ctcagccacg cccaaaataa tgctgatacc tacagtggag acagcagagc
     4921 cggcctcgtc cagtacatcc actgactccg ccaactggag ctggattttt ggcaccacag
     4981 tgagaatagc aaggacatcc aaagcaaaac gcacagtatc attccttgca taataggtct
     5041 tccaattgca ggcgatggaa ataagctgta gtaaaagctg cacacaggaa agcttgagga
     5101 agacttcagc aggttcccaa tatagctgag ctgggccata ctctatcaaa aattccatca
     5161 tttctacaat ctgctcatga gtatatgagc atgccttgta tggaggctgg gggtgaacaa
     5221 gaataccacc ctcagtcctc tgaagtgact gcttgacttg ttccaattta atggccagat
     5281 gagcctcaaa atacttgcgc agagccatgc aggtatgttt tcctgtttgg cgactagcaa
     5341 atatttcatc atcactcaaa agtgcaccct ggtcttccaa atttaaaatc tccaaagtac
     5401 tgatcaagtt caccagacga cgaagaccat cataacggtc aaagagctcc aaaacagccc
     5461 ggaatgagaa acaaattgag aaaaacatag tagcatggca acatcctgaa gcatgagaac
     5521 attccattaa ccacagggta tagttcacca catcagatag aacattgtga ggatgcatgc
     5581 aaactctttc catagcatcc tgattgtagg acaggtaata caagcacatg gacacaccag
     5641 ttgcagccat agaaggacga ggaatttcca gtaatttctg tactccacca tgtgcaacaa
     5701 attctgtggc aaatttgtta tgaagcagga gagatgctag gtgcttgagt gcctcaaaag
     5761 taagcaggac atcattcgtc tgtttcagat caatataaaa catcatcaat tctcttgatc
     5821 caagttgcat gaatatggga agtaactcct gatactctcc taaaggggtc aaatactgaa
     5881 gaataagtct ctgctcaata gcaggagtca taggatagag agtgtaattg gtgccgatca
     5941 cccaggggga catttctgac cagctgctat tagacagctc aacaaacaca cgatctgggt
     6001 cagaagatga gaagcccaac ttctgcttgg ccttcctaaa gttctctctg tcaccatgct
     6061 ttgctgactt gtttttcttt aatcctcctt cctcaggttt agttgctgag ttcactctac
     6121 tactggtctt gtgacttgaa tcaagacgaa aggagatctc catatctcta gaagattctt
     6181 gttctccatc cacaacatcc actgccatgt caccatagtc catatccaca gcttcctcat
     6241 ccagaggcaa aaggggttcc gaagagagct tccgtggact aggacgctta ctgtcctgcc
     6301 gcaaagccac ttcttgaagc tggagctccc tcagtcttct aagcactatt gccaccaact
     6361 gtgaattttc atctctgtag ttggcagcaa tatcttgatt ttccatagca cctcccagca
     6421 gtccagtaga atatgtccta agtggctgat cagcctctcg ggcccatttg aaaagatttt
     6481 caacaattcc ttccttttct tgaaagacaa ctgcggtttc taggcccggc atgatgtcca
     6541 gcagcagtct gcaagctgca gtatttaaag ggggctctct acttgtcatc acatatgcat
     6601 ttaccagcgc attcatgaaa tcatcattct tgaagagtat tctcagcaaa tggcccagca
     6661 tacactctgg atcagctcga ccaggatgtc gatcatcaaa tggatctgga tctcctttac
     6721 gatattcttc agtttctttt tcaattaatt cagacattct ggtaaggatt ggtaccatat
     6781 cctggccact gccatggtcc ttttcccact gctccagcag ggtagtgagc tcagctttgg
     6841 agtccacatg taccactact gtagtcatgc gatcgcccat ggtggcgtcc acggcgctgt
     6901 tgctcgcctg ggctctgctc ctcaggccgc tcttgtacag ctcgtccatg ccgccggtgg
     6961 agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg
     7021 tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct
     7081 tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct
     7141 tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc
     7201 ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca
     7261 ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc
     7321 cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt
     7381 agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg
     7441 acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct
     7501 gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca
     7561 cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct
     7621 cctcgccctt gctcaccatg gtggcgggat ccgaattctt tgccaaaatg atgagacagc
     7681 acaataacca gcacgttgcc caggagctgt aggaaaaaga agaaggcatg aacatggtta
     7741 gcagaggctc tagagccgcc ggtcacacgc cagaagccga accccgccct gccccgtccc
     7801 ccccgaaggc agccgtcccc ccgcggacag ccccgaggct ggagagggag aaggggacgg
     7861 cggcgcggcg acgcacgaag gccctccccg cccatttcct tcctgccggc gccgcaccgc
     7921 ttcgccccgc gcccgctaga gggggtgcgg cggcgcctcc cagatttcgg ctccgcacag
     7981 atttgggaca aaggaagtcc ctgcgccctc tcgcacgatt accataaaag gcaatggctg
     8041 cggctcgccg cgcctcgaca gccgccggcg ctccgggggc cgccgcgccc ctcccccgag
     8101 ccctccccgg cccgaggcgg ccccgccccg cccggcaccc ccacctgccg ccaccccccg
     8161 cccggcacgg cgagccccgc gccacgcccc gtacggagcc ccgcacccga agccgggccg
     8221 tgctcagcaa ctcggggagg ggggtgcagg gggggttgca gcccgaccga cgcgcccaca
     8281 ccccctgctc acccccccac gcacacaccc cgcacgcagc ctttgttccc ctcgcagccc
     8341 cccccgcacc gcggggcacc gcccccggcc gcgctcccct cgcgcacact gcggagcgca
     8401 caaagccccg cgccgcgccc gcagcgctca cagccgccgg gcagcgcgga gccgcacgcg
     8461 gcgctcccca cgcacacaca cacgcacgca ccccccgagc cgctcccccc gcacaaaggg
     8521 ccctcccgga gcccctcaag gctttcacgc agccacagaa aagaaacaag ccgtcattaa
     8581 accaagcgct aattacagcc cggaggagaa gggccgtccc gcccgctcac ctgtgggagt
     8641 aacgcggtca gtcagagccg gggcgggcgg cgcgaggcgg cggcggagcg gggcacgggg
     8701 cgaaggcagc gcgcagcgac tcccgcccgc cgcgcgcttc gctttttata gggccgccgc
     8761 cgccgccgcc tcgccataaa aggaaacttt cggagcgcgc cgctctgatt ggctgccgcc
     8821 gcacctctcc gcctcgcccc gccccgcccc tcgccccgcc ccgccccgcc tggcgcgcgc
     8881 cccccccccc ccccccgccc ccatcgctgc acaaaataat taaaaaataa ataaatacaa
     8941 aattgggggt ggggaggggg gggagatggg gagagtgaag cagaacgtgg ggctcacctc
     9001 gaccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     9061 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     9121 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     9181 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     9241 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     9301 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     9361 ataactagga cgcgtcctat agtgagtcgt attacaattc actggccgtc gttttacaac
     9421 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     9481 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     9541 gcctgaatgg cgaatggacg cgccctgtag cggcgcattg agcgcggcgg gtgtggtggt
     9601 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     9661 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     9721 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     9781 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     9841 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     9901 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     9961 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg
    10021 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
    10081 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
    10141 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
    10201 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
    10261 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
    10321 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    10381 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
    10441 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
    10501 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
    10561 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
    10621 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
    10681 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    10741 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    10801 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    10861 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    10921 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    10981 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    11041 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    11101 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    11161 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    11221 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    11281 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    11341 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    11401 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    11461 gaaaagatca aaggatcttc
//
