LOCUS       sequence-436314-       11492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses of GFP-tagged VprBP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, 
            Ludwig AK, Carell T, Cardoso MC
  TITLE     Isoform-specific and ubiquitination dependent recruitment of Tet1 to
            replicating heterochromatin modulates methylcytosine oxidation.
  JOURNAL   Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.
  PUBMED    36056023
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-2"; volume: "13(1):5173. doi"; pages: " 
            10.1038/s41467-022-32799-8"
COMMENT     Sequence Label: pGFP-mVprBP  pc2953
FEATURES             Location/Qualifiers
     source          1..11492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      763..772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1989..2000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    5444..5906
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5500..5517)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5727..5746
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5911..5920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5917..6315
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6473..6594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6510..6529)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6564..6583
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6642..6777
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6883..6900)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7054..7642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7134..7153)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7813..8604,8605..8673))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8436..8455
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8674..8778)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8846..8864
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8902..8924)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9024..9043
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9110..9565)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9247..9268)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9459..9478
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9691..9713
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9705..9722
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9706..9722
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9729..9747
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        9816..10119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10121..10398
                     /label=chicken beta-actin promoter
     intron          10399..11415
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     11339..11361
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     11423..11442
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      join(11487..11492,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagc
      721 ggcctgagga gcagagccca ggcgagcaac agcgccgtgg acgccaccat gggcgatcgc
      781 atgactacag tagtggtaca tgtggactcc aaagctgagc tcactaccct gctggagcag
      841 tgggaaaagg accatggcag tggccaggat atggtaccaa tccttaccag aatgtctgaa
      901 ttaattgaaa aagaaactga agaatatcgt aaaggagatc cagatccatt tgatgatcga
      961 catcctggtc gagctgatcc agagtgtatg ctgggccatt tgctgagaat actcttcaag
     1021 aatgatgatt tcatgaatgc gctggtaaat gcatatgtga tgacaagtag agagccccct
     1081 ttaaatactg cagcttgcag actgctgctg gacatcatgc cgggcctaga aaccgcagtt
     1141 gtctttcaag aaaaggaagg aattgttgaa aatcttttca aatgggcccg agaggctgat
     1201 cagccactta ggacatattc tactggactg ctgggaggtg ctatggaaaa tcaagatatt
     1261 gctgccaact acagagatga aaattcacag ttggtggcaa tagtgcttag aagactgagg
     1321 gagctccagc ttcaagaagt ggctttgcgg caggacagta agcgtcctag tccacggaag
     1381 ctctcttcgg aacccctttt gcctctggat gaggaagctg tggatatgga ctatggtgac
     1441 atggcagtgg atgttgtgga tggagaacaa gaatcttcta gagatatgga gatctccttt
     1501 cgtcttgatt caagtcacaa gaccagtagt agagtgaact cagcaactaa acctgaggaa
     1561 ggaggattaa agaaaaacaa gtcagcaaag catggtgaca gagagaactt taggaaggcc
     1621 aagcagaagt tgggcttctc atcttctgac ccagatcgtg tgtttgttga gctgtctaat
     1681 agcagctggt cagaaatgtc cccctgggtg atcggcacca attacactct ctatcctatg
     1741 actcctgcta ttgagcagag acttattctt cagtatttga cccctttagg agagtatcag
     1801 gagttacttc ccatattcat gcaacttgga tcaagagaat tgatgatgtt ttatattgat
     1861 ctgaaacaga cgaatgatgt cctgcttact tttgaggcac tcaagcacct agcatctctc
     1921 ctgcttcata acaaatttgc cacagaattt gttgcacatg gtggagtaca gaaattactg
     1981 gaaattcctc gtccttctat ggctgcaact ggtgtgtcca tgtgcttgta ttacctgtcc
     2041 tacaatcagg atgctatgga aagagtttgc atgcatcctc acaatgttct atctgatgtg
     2101 gtgaactata ccctgtggtt aatggaatgt tctcatgctt caggatgttg ccatgctact
     2161 atgtttttct caatttgttt ctcattccgg gctgttttgg agctctttga ccgttatgat
     2221 ggtcttcgtc gtctggtgaa cttgatcagt actttggaga ttttaaattt ggaagaccag
     2281 ggtgcacttt tgagtgatga tgaaatattt gctagtcgcc aaacaggaaa acatacctgc
     2341 atggctctgc gcaagtattt tgaggctcat ctggccatta aattggaaca agtcaagcag
     2401 tcacttcaga ggactgaggg tggtattctt gttcaccccc agcctccata caaggcatgc
     2461 tcatatactc atgagcagat tgtagaaatg atggaatttt tgatagagta tggcccagct
     2521 cagctatatt gggaacctgc tgaagtcttc ctcaagcttt cctgtgtgca gcttttacta
     2581 cagcttattt ccatcgcctg caattggaag acctattatg caaggaatga tactgtgcgt
     2641 tttgctttgg atgtccttgc tattctcact gtggtgccaa aaatccagct ccagttggcg
     2701 gagtcagtgg atgtactgga cgaggccggc tctgctgtct ccactgtagg tatcagcatt
     2761 attttgggcg tggctgaggg tgagttcttt atccatgatg ctgaaattca gaagtcagca
     2821 cttcaaatta tcatcaattg tgtatgtggt ccagataacc gaatatccag tattggcaaa
     2881 tttatttctg gaactcctcg gagaaagctg cctcagactc ccaagagtag tgagcatact
     2941 ctggccaaga tgtggaatgt ggtccagtcc aacaatggca tcaaggtgct tctgtccctg
     3001 ttgtccatca agatgcccat tacagacgca gaccagatcc gggccctggc ctgcaaagcc
     3061 cttgtaggcc tgtctcgaag tagcactgtt cgacagatca tcagtaaact tccccttttt
     3121 agtagctgcc agattcaaca gctgatgaaa gagcctgtgc tgcaggataa gcgtagtgac
     3181 catgtcaagt tctgcaaata cgctgctgag ctcattgagc gtgtatcagg aaagcctctt
     3241 ctcattggca ctgatgtgtc tctcgcacga ctgcagaaag cagatgttgt tgctcagtcg
     3301 aggatctcct tccctgagaa agagctgctc ctgttgatac ggaaccatct catttctaaa
     3361 gggcttggag agacagcaac tgtgctgacg agagaggctg acctgcccat gactgctgcc
     3421 tcacattctt ctgccttcac cccagtgact gctgctgctt ctcctgtctc tcttccccgg
     3481 acccctcgga ttgccaacgg cattgcaagt cgactgggca gccatgctac tgtgggtgct
     3541 tctgcccctt ctgctccccc tgcccatcct ccaccacggc ccccccaggg ttcattgcct
     3601 ttgcctggcc catcatatgc aggcaattcc cctttgattg gtaggatcag ttttatcaga
     3661 gagaggccat caccgtgcaa tggcaggaaa attcgagtgt tgcggcagaa gtcggaccat
     3721 ggtgcataca gccagagccc tgccataaaa aaacaattgg atagacatct tccttcccca
     3781 cctacgctgg acagtatcat cacagagtat cttagagagc aacatgctcg atgcaagaac
     3841 cccgttgcca cctgtcctcc tttttctctc tttactcctc atcagtgtcc tgaaccaaaa
     3901 cagaggcggc aagcgccaat aaactttacc tcaaggctaa accgcagggc atcatttcca
     3961 aaatatggag gggtagatgg cggatgcttt gataggcacc ttatctttag cagatttcgt
     4021 ccgatttcag tattccggga agctaatgaa gatgaaagtg gcttcacctg ttgtgcattc
     4081 tcagcccggg agcggttcct gatgcttggc acttgtacag ggcagctgaa gctctacaat
     4141 gtgtttagtg gacaggaaga ggccagctat aactgtcaca actcagccat cacacacctt
     4201 gaaccgtcaa gggatgggtc cttacttctg acatctgcca cttggagcca gcctttgtct
     4261 gcactttggg gaatgaaatc agtatttgat atgaagcatt ccttcacaga agatcactat
     4321 gttgagttca gtaagcactc tcaggatcgg gtcataggca caaaaggaga cattgcccat
     4381 atttatgata ttcagactgg caacaagctg ttgactctgt ttaacccaga tcttgccaac
     4441 aactacaaga ggaactgtgc cacctttaat cctacagatg atcttgtctt aaatgatggc
     4501 gtcctctggg atgtccgctc tgcacaggcc atccacaagt ttgacaagtt caacatgaat
     4561 atcagtggtg ttttccatcc aaatgggctg gaagtcatca tcaatacaga gatttgggac
     4621 cttcggactt tccatctttt acacactgtt cctgctctgg atcaatgccg tgttgttttt
     4681 aaccacactg ggacagtgat gtatggagca atgttgcagg cagatgatga agatgactta
     4741 ctggaagaga ggatgaaaag cccttttggg tcatcctttc gaacattcaa cgcaactgat
     4801 tataaaccta tagcaactat tgatgtgaaa cgaaacatct ttgacctgtg cacagacacc
     4861 aaagactgtt atcttgctgt cattgagaat caaggcagca tggatgcact gaatatggac
     4921 acagtgtgca ggctctatga agtgggcagg cagcgtctgg cagaggatga ggatgaagag
     4981 gaggaccagg aagaggaaga acaggaggaa gaagatgatg atgaagatga tgatgatact
     5041 gatgatttag atgaactaga cactgaccag ttgctggagg cagaactgga ggaggatgac
     5101 aacaatgaga atgcaggaga ggatggagac aatgacttct ctccctctga tgaggagcta
     5161 gcaaaccttc tagaggaggg agaggagggg gaagatgaag actctgatgc agatgaggag
     5221 gtggagctga tcctggggga cacagacagc tcggacaact ctgatttgga agatgacatc
     5281 atcttatctc tgaatgagtg atgcggccgc agttaacgaa ttcactcgag cgggatcaat
     5341 tcccccccsc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5401 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5461 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5521 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5581 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5641 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5701 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5761 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5821 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5881 ttttcctttg aaaaacacga tgataagctt gccaccatgg ccaagccttt gtctcaagaa
     5941 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
     6001 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
     6061 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
     6121 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
     6181 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
     6241 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
     6301 gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     6361 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6421 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6481 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6541 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6601 ctgtataccg tcgacctcga ggagcttttt gcaaaagcct aggcctccaa aaaagcctct
     6661 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     6721 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     6781 cggagttagg ggcgggacta tggttgctga ctaattgctg cattaatgaa tcggccaacg
     6841 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6901 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6961 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7021 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7081 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7141 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7201 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7261 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7321 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7381 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7441 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7501 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7561 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7621 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7681 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7741 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7801 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7861 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7921 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7981 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8041 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8101 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8161 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8221 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8281 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8341 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8401 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8461 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8521 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8581 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8641 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8701 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8761 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     8821 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     8881 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     8941 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     9001 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     9061 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc
     9121 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     9181 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     9241 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     9301 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     9361 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     9421 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     9481 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     9541 ctcaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     9601 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     9661 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     9721 gtgaattgta atacgactca ctataggacg cgtcctagtt attaatagta atcaattacg
     9781 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     9841 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     9901 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     9961 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
    10021 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
    10081 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
    10141 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
    10201 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg
    10261 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
    10321 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
    10381 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
    10441 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
    10501 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
    10561 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
    10621 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
    10681 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
    10741 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
    10801 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
    10861 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
    10921 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
    10981 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
    11041 ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg
    11101 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct
    11161 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg
    11221 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc
    11281 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
    11341 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
    11401 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
    11461 aaagaattcg gatccaccgg ccggtcgcca cc
//
